Chitopentaose protects HaCaT cells against H2O2-induced oxidative damage through modulating MAPKs and Nrf2/ARE signaling pathways
Chito-oligosaccharides, the novel marine functional oligosaccharides, possess broad activities including anti-inflammatory and anti-oxidative effects. However, there are few studies about the molecular mechanisms of chito-oligosaccharides monomers against oxidative stress. In this study, the anti-ox...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-09-01
|
| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464620303108 |
| _version_ | 1818410536298086400 |
|---|---|
| author | Jing Zhang Wei Wang Xiangzhao Mao |
| author_facet | Jing Zhang Wei Wang Xiangzhao Mao |
| author_sort | Jing Zhang |
| collection | DOAJ |
| description | Chito-oligosaccharides, the novel marine functional oligosaccharides, possess broad activities including anti-inflammatory and anti-oxidative effects. However, there are few studies about the molecular mechanisms of chito-oligosaccharides monomers against oxidative stress. In this study, the anti-oxidative effect and mechanism of Chitopentaose hydrochloride (COS-5) were explored in HaCaT cells. The results indicated that COS-5 improved cell viability and decreased the production of ROS induced by hydrogen peroxide (H2O2). COS-5 can increase the antioxidant-related enzymes including SOD and CAT and can reduce MDA production and LDH leakage in HaCaT cells. COS-5 effectively inhibited the decline of MMP and the expression of Caspase-3 protein. Moreover, COS-5 can significantly down-regulate abnormal activation of MAPKs pathway and up-regulate the Nrf2/ARE pathway. In summary, COS-5 has significant protective effect against H2O2-induced oxidative stress in HaCaT cells through regulating MAPKs and Nrf2/ARE pathways, suggesting that COS-5 may be used as a novel functional food supplement to prevent oxidative-stress-related diseases in the future. |
| first_indexed | 2024-12-14T10:17:05Z |
| format | Article |
| id | doaj.art-d6bd39f7396f4dab8631e55fbd012b90 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T10:17:05Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-d6bd39f7396f4dab8631e55fbd012b902022-12-21T23:06:47ZengElsevierJournal of Functional Foods1756-46462020-09-0172104086Chitopentaose protects HaCaT cells against H2O2-induced oxidative damage through modulating MAPKs and Nrf2/ARE signaling pathwaysJing Zhang0Wei Wang1Xiangzhao Mao2College of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaKey Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; Corresponding authors at: College of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao 266003, China (W. Wang). College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao 266003, China (X. Mao).College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; Corresponding authors at: College of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao 266003, China (W. Wang). College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao 266003, China (X. Mao).Chito-oligosaccharides, the novel marine functional oligosaccharides, possess broad activities including anti-inflammatory and anti-oxidative effects. However, there are few studies about the molecular mechanisms of chito-oligosaccharides monomers against oxidative stress. In this study, the anti-oxidative effect and mechanism of Chitopentaose hydrochloride (COS-5) were explored in HaCaT cells. The results indicated that COS-5 improved cell viability and decreased the production of ROS induced by hydrogen peroxide (H2O2). COS-5 can increase the antioxidant-related enzymes including SOD and CAT and can reduce MDA production and LDH leakage in HaCaT cells. COS-5 effectively inhibited the decline of MMP and the expression of Caspase-3 protein. Moreover, COS-5 can significantly down-regulate abnormal activation of MAPKs pathway and up-regulate the Nrf2/ARE pathway. In summary, COS-5 has significant protective effect against H2O2-induced oxidative stress in HaCaT cells through regulating MAPKs and Nrf2/ARE pathways, suggesting that COS-5 may be used as a novel functional food supplement to prevent oxidative-stress-related diseases in the future.http://www.sciencedirect.com/science/article/pii/S1756464620303108AntioxidantChitopentaose hydrochlorideH2O2HaCaT cellsMAPKs pathwayNrf2/ARE pathway |
| spellingShingle | Jing Zhang Wei Wang Xiangzhao Mao Chitopentaose protects HaCaT cells against H2O2-induced oxidative damage through modulating MAPKs and Nrf2/ARE signaling pathways Journal of Functional Foods Antioxidant Chitopentaose hydrochloride H2O2 HaCaT cells MAPKs pathway Nrf2/ARE pathway |
| title | Chitopentaose protects HaCaT cells against H2O2-induced oxidative damage through modulating MAPKs and Nrf2/ARE signaling pathways |
| title_full | Chitopentaose protects HaCaT cells against H2O2-induced oxidative damage through modulating MAPKs and Nrf2/ARE signaling pathways |
| title_fullStr | Chitopentaose protects HaCaT cells against H2O2-induced oxidative damage through modulating MAPKs and Nrf2/ARE signaling pathways |
| title_full_unstemmed | Chitopentaose protects HaCaT cells against H2O2-induced oxidative damage through modulating MAPKs and Nrf2/ARE signaling pathways |
| title_short | Chitopentaose protects HaCaT cells against H2O2-induced oxidative damage through modulating MAPKs and Nrf2/ARE signaling pathways |
| title_sort | chitopentaose protects hacat cells against h2o2 induced oxidative damage through modulating mapks and nrf2 are signaling pathways |
| topic | Antioxidant Chitopentaose hydrochloride H2O2 HaCaT cells MAPKs pathway Nrf2/ARE pathway |
| url | http://www.sciencedirect.com/science/article/pii/S1756464620303108 |
| work_keys_str_mv | AT jingzhang chitopentaoseprotectshacatcellsagainsth2o2inducedoxidativedamagethroughmodulatingmapksandnrf2aresignalingpathways AT weiwang chitopentaoseprotectshacatcellsagainsth2o2inducedoxidativedamagethroughmodulatingmapksandnrf2aresignalingpathways AT xiangzhaomao chitopentaoseprotectshacatcellsagainsth2o2inducedoxidativedamagethroughmodulatingmapksandnrf2aresignalingpathways |