Carbon and Nitrogen Sources Have No Impact on the Organization and Composition of <i>Ustilago maydis</i> Respiratory Supercomplexes
Respiratory supercomplexes are found in mitochondria of eukaryotic cells and some bacteria. A hypothetical role of these supercomplexes is electron channeling, which in principle should increase the respiratory chain efficiency and ATP synthesis. In addition to the four classic respiratory complexes...
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MDPI AG
2021-01-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/1/42 |
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| author | Deyamira Matuz-Mares Oscar Flores-Herrera Guadalupe Guerra-Sánchez Lucero Romero-Aguilar Héctor Vázquez-Meza Genaro Matus-Ortega Federico Martínez Juan Pablo Pardo |
| author_facet | Deyamira Matuz-Mares Oscar Flores-Herrera Guadalupe Guerra-Sánchez Lucero Romero-Aguilar Héctor Vázquez-Meza Genaro Matus-Ortega Federico Martínez Juan Pablo Pardo |
| author_sort | Deyamira Matuz-Mares |
| collection | DOAJ |
| description | Respiratory supercomplexes are found in mitochondria of eukaryotic cells and some bacteria. A hypothetical role of these supercomplexes is electron channeling, which in principle should increase the respiratory chain efficiency and ATP synthesis. In addition to the four classic respiratory complexes and the ATP synthase, <i>U. maydis</i> mitochondria contain three type II NADH dehydrogenases (NADH for reduced nicotinamide adenine dinucleotide) and the alternative oxidase. Changes in the composition of the respiratory supercomplexes due to energy requirements have been reported in certain organisms. In this study, we addressed the organization of the mitochondrial respiratory complexes in <i>U. maydis</i> under diverse energy conditions. Supercomplexes were obtained by solubilization of <i>U. maydis</i> mitochondria with digitonin and separated by blue native polyacrylamide gel electrophoresis (BN-PAGE). The molecular mass of supercomplexes and their probable stoichiometries were 1200 kDa (I<sub>1</sub>:IV<sub>1</sub>), 1400 kDa (I<sub>1</sub>:III<sub>2</sub>), 1600 kDa (I<sub>1</sub>:III<sub>2</sub>:IV<sub>1</sub>), and 1800 kDa (I<sub>1</sub>:III<sub>2</sub>:IV<sub>2</sub>). Concerning the ATP synthase, approximately half of the protein is present as a dimer and half as a monomer. The distribution of respiratory supercomplexes was the same in all growth conditions. We did not find evidence for the association of complex II and the alternative NADH dehydrogenases with other respiratory complexes. |
| first_indexed | 2024-03-09T05:12:22Z |
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| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T05:12:22Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-d6be705f93d34e198c35d9951a5500792023-12-03T12:48:40ZengMDPI AGJournal of Fungi2309-608X2021-01-01714210.3390/jof7010042Carbon and Nitrogen Sources Have No Impact on the Organization and Composition of <i>Ustilago maydis</i> Respiratory SupercomplexesDeyamira Matuz-Mares0Oscar Flores-Herrera1Guadalupe Guerra-Sánchez2Lucero Romero-Aguilar3Héctor Vázquez-Meza4Genaro Matus-Ortega5Federico Martínez6Juan Pablo Pardo7Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Copilco, Cd. Universitaria, Coyoacán, Ciudad de México 04510, MexicoDepartamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Copilco, Cd. Universitaria, Coyoacán, Ciudad de México 04510, MexicoLaboratorio de Bioquímica y Biotecnología de Hongos, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala S/N, Miguel Hidalgo, Ciudad de México 11350, MexicoDepartamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Copilco, Cd. Universitaria, Coyoacán, Ciudad de México 04510, MexicoDepartamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Copilco, Cd. Universitaria, Coyoacán, Ciudad de México 04510, MexicoDepartamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Copilco, Cd. Universitaria, Coyoacán, Ciudad de México 04510, MexicoDepartamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Copilco, Cd. Universitaria, Coyoacán, Ciudad de México 04510, MexicoDepartamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Avenida Universidad 3000, Copilco, Cd. Universitaria, Coyoacán, Ciudad de México 04510, MexicoRespiratory supercomplexes are found in mitochondria of eukaryotic cells and some bacteria. A hypothetical role of these supercomplexes is electron channeling, which in principle should increase the respiratory chain efficiency and ATP synthesis. In addition to the four classic respiratory complexes and the ATP synthase, <i>U. maydis</i> mitochondria contain three type II NADH dehydrogenases (NADH for reduced nicotinamide adenine dinucleotide) and the alternative oxidase. Changes in the composition of the respiratory supercomplexes due to energy requirements have been reported in certain organisms. In this study, we addressed the organization of the mitochondrial respiratory complexes in <i>U. maydis</i> under diverse energy conditions. Supercomplexes were obtained by solubilization of <i>U. maydis</i> mitochondria with digitonin and separated by blue native polyacrylamide gel electrophoresis (BN-PAGE). The molecular mass of supercomplexes and their probable stoichiometries were 1200 kDa (I<sub>1</sub>:IV<sub>1</sub>), 1400 kDa (I<sub>1</sub>:III<sub>2</sub>), 1600 kDa (I<sub>1</sub>:III<sub>2</sub>:IV<sub>1</sub>), and 1800 kDa (I<sub>1</sub>:III<sub>2</sub>:IV<sub>2</sub>). Concerning the ATP synthase, approximately half of the protein is present as a dimer and half as a monomer. The distribution of respiratory supercomplexes was the same in all growth conditions. We did not find evidence for the association of complex II and the alternative NADH dehydrogenases with other respiratory complexes.https://www.mdpi.com/2309-608X/7/1/42respiratory complexesmitochondrial supercomplexes<i>Ustilago maydis</i> mitochondria |
| spellingShingle | Deyamira Matuz-Mares Oscar Flores-Herrera Guadalupe Guerra-Sánchez Lucero Romero-Aguilar Héctor Vázquez-Meza Genaro Matus-Ortega Federico Martínez Juan Pablo Pardo Carbon and Nitrogen Sources Have No Impact on the Organization and Composition of <i>Ustilago maydis</i> Respiratory Supercomplexes Journal of Fungi respiratory complexes mitochondrial supercomplexes <i>Ustilago maydis</i> mitochondria |
| title | Carbon and Nitrogen Sources Have No Impact on the Organization and Composition of <i>Ustilago maydis</i> Respiratory Supercomplexes |
| title_full | Carbon and Nitrogen Sources Have No Impact on the Organization and Composition of <i>Ustilago maydis</i> Respiratory Supercomplexes |
| title_fullStr | Carbon and Nitrogen Sources Have No Impact on the Organization and Composition of <i>Ustilago maydis</i> Respiratory Supercomplexes |
| title_full_unstemmed | Carbon and Nitrogen Sources Have No Impact on the Organization and Composition of <i>Ustilago maydis</i> Respiratory Supercomplexes |
| title_short | Carbon and Nitrogen Sources Have No Impact on the Organization and Composition of <i>Ustilago maydis</i> Respiratory Supercomplexes |
| title_sort | carbon and nitrogen sources have no impact on the organization and composition of i ustilago maydis i respiratory supercomplexes |
| topic | respiratory complexes mitochondrial supercomplexes <i>Ustilago maydis</i> mitochondria |
| url | https://www.mdpi.com/2309-608X/7/1/42 |
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