Isolation, Molecular Identification and Amino Acid Profiling of Single-Cell-Protein-Producing Phototrophic Bacteria Isolated from Oil-Contaminated Soil Samples
In the current study, soil samples were gathered from different places where petrol and diesel filling stations were located for isolation of photosynthetic bacteria under anaerobic conditions using the paraffin wax-overlay pour plate method with Biebl and Pfennig’s medium. The three isolated strain...
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MDPI AG
2022-09-01
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| author | Raju Nalvothula Surekha Challa Vidyullatha Peddireddy Ramchander Merugu M. P. Pratap Rudra Abed Alataway Ahmed Z. Dewidar Hosam O. Elansary |
| author_facet | Raju Nalvothula Surekha Challa Vidyullatha Peddireddy Ramchander Merugu M. P. Pratap Rudra Abed Alataway Ahmed Z. Dewidar Hosam O. Elansary |
| author_sort | Raju Nalvothula |
| collection | DOAJ |
| description | In the current study, soil samples were gathered from different places where petrol and diesel filling stations were located for isolation of photosynthetic bacteria under anaerobic conditions using the paraffin wax-overlay pour plate method with Biebl and Pfennig’s medium. The three isolated strains were named <i>Rhodopseudomonas palustris</i> SMR 001 (Mallapur), <i>Rhodopseudomonas palustris</i> NR MPPR (Nacahram) and <i>Rhodopseudomonas faecalis</i> N Raju MPPR (Karolbagh). The morphologies of the bacteria were examined with a scanning electron microscope (SEM). The phylogenetic relationship between <i>R. palustris</i> strains was examined by means of 16S rRNA gene sequence analysis using NCBI-BLAST search and a phylogenetic tree. The sequenced data for <i>R. palustris</i> were deposited with the National Centre for Biotechnology Research (NCBI). The total amino acids produced by the isolated bacteria were determined by HPLC. A total of 14 amino acids and their derivatives were produced by the <i>R. palustris</i> SMR 001 strain. Among these, carnosine was found in the highest concentration (8553.2 ng/mL), followed by isoleucine (1818.044 ng/mL) and anserine (109.5 ng/mL), while <i>R. palustris</i> NR MPPR was found to produce 12 amino acids. Thirteen amino acids and their derivatives were found to be produced from <i>R. faecalis</i> N Raju MPPR, for which the concentration of carnosine (21601.056 ng/mL) was found to be the highest, followed by isoleucine (2032.6 ng/mL) and anserine (227.4 ng/mL). These microbes can be explored for the scaling up of the process, along with biohydrogen and single cell protein production. |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T21:26:04Z |
| publishDate | 2022-09-01 |
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| spelling | doaj.art-d6beda9afc3646a8a89efaccf22f77e72023-11-23T21:08:29ZengMDPI AGMolecules1420-30492022-09-012719626510.3390/molecules27196265Isolation, Molecular Identification and Amino Acid Profiling of Single-Cell-Protein-Producing Phototrophic Bacteria Isolated from Oil-Contaminated Soil SamplesRaju Nalvothula0Surekha Challa1Vidyullatha Peddireddy2Ramchander Merugu3M. P. Pratap Rudra4Abed Alataway5Ahmed Z. Dewidar6Hosam O. Elansary7Department of Biochemistry, Osmania University, Hyderabad 500007, IndiaDepartment of Biochemistry and Bioinformatics, GSS, GITAM, A P., Gandhinagar 530045, IndiaDepartment of Nutrition Biology, School of Interdisciplinary & Applied Sciences, Central University of Haryana, Jant-Pali, Mahendergarh 123031, IndiaDepartment of Biochemistry, Mahatma Gandhi University, Nalgonda 508254, IndiaDepartment of Biochemistry, Osmania University, Hyderabad 500007, IndiaPrince Sultan Bin Abdulaziz International Prize for Water Chair, Prince Sultan Institute for Environmental, Water and Desert Research, King Saud University, Riyadh 11451, Saudi ArabiaPrince Sultan Bin Abdulaziz International Prize for Water Chair, Prince Sultan Institute for Environmental, Water and Desert Research, King Saud University, Riyadh 11451, Saudi ArabiaPrince Sultan Bin Abdulaziz International Prize for Water Chair, Prince Sultan Institute for Environmental, Water and Desert Research, King Saud University, Riyadh 11451, Saudi ArabiaIn the current study, soil samples were gathered from different places where petrol and diesel filling stations were located for isolation of photosynthetic bacteria under anaerobic conditions using the paraffin wax-overlay pour plate method with Biebl and Pfennig’s medium. The three isolated strains were named <i>Rhodopseudomonas palustris</i> SMR 001 (Mallapur), <i>Rhodopseudomonas palustris</i> NR MPPR (Nacahram) and <i>Rhodopseudomonas faecalis</i> N Raju MPPR (Karolbagh). The morphologies of the bacteria were examined with a scanning electron microscope (SEM). The phylogenetic relationship between <i>R. palustris</i> strains was examined by means of 16S rRNA gene sequence analysis using NCBI-BLAST search and a phylogenetic tree. The sequenced data for <i>R. palustris</i> were deposited with the National Centre for Biotechnology Research (NCBI). The total amino acids produced by the isolated bacteria were determined by HPLC. A total of 14 amino acids and their derivatives were produced by the <i>R. palustris</i> SMR 001 strain. Among these, carnosine was found in the highest concentration (8553.2 ng/mL), followed by isoleucine (1818.044 ng/mL) and anserine (109.5 ng/mL), while <i>R. palustris</i> NR MPPR was found to produce 12 amino acids. Thirteen amino acids and their derivatives were found to be produced from <i>R. faecalis</i> N Raju MPPR, for which the concentration of carnosine (21601.056 ng/mL) was found to be the highest, followed by isoleucine (2032.6 ng/mL) and anserine (227.4 ng/mL). These microbes can be explored for the scaling up of the process, along with biohydrogen and single cell protein production.https://www.mdpi.com/1420-3049/27/19/6265phototrophic bacteriamolecular characterization16S rRNA gene sequenceamino acid profilingSEMHPLC |
| spellingShingle | Raju Nalvothula Surekha Challa Vidyullatha Peddireddy Ramchander Merugu M. P. Pratap Rudra Abed Alataway Ahmed Z. Dewidar Hosam O. Elansary Isolation, Molecular Identification and Amino Acid Profiling of Single-Cell-Protein-Producing Phototrophic Bacteria Isolated from Oil-Contaminated Soil Samples Molecules phototrophic bacteria molecular characterization 16S rRNA gene sequence amino acid profiling SEM HPLC |
| title | Isolation, Molecular Identification and Amino Acid Profiling of Single-Cell-Protein-Producing Phototrophic Bacteria Isolated from Oil-Contaminated Soil Samples |
| title_full | Isolation, Molecular Identification and Amino Acid Profiling of Single-Cell-Protein-Producing Phototrophic Bacteria Isolated from Oil-Contaminated Soil Samples |
| title_fullStr | Isolation, Molecular Identification and Amino Acid Profiling of Single-Cell-Protein-Producing Phototrophic Bacteria Isolated from Oil-Contaminated Soil Samples |
| title_full_unstemmed | Isolation, Molecular Identification and Amino Acid Profiling of Single-Cell-Protein-Producing Phototrophic Bacteria Isolated from Oil-Contaminated Soil Samples |
| title_short | Isolation, Molecular Identification and Amino Acid Profiling of Single-Cell-Protein-Producing Phototrophic Bacteria Isolated from Oil-Contaminated Soil Samples |
| title_sort | isolation molecular identification and amino acid profiling of single cell protein producing phototrophic bacteria isolated from oil contaminated soil samples |
| topic | phototrophic bacteria molecular characterization 16S rRNA gene sequence amino acid profiling SEM HPLC |
| url | https://www.mdpi.com/1420-3049/27/19/6265 |
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