Comparison of Various Solvent Extracts and Major Bioactive Components from <i>Portulaca oleracea</i> for Antioxidant, Anti-Tyrosinase, and Anti-α-Glucosidase Activities
<i>Portulaca oleracea</i> is a well-known species for traditional medicine and food homology in Taiwan. In traditional medicine, <i>P. oleracea</i> is also used to treat gastrointestinal disorders, liver inflammation, fever, severe inflammation, and headaches. We investigated...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/2/398 |
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| author | Wei-Cheng Chen Shih-Wei Wang Cai-Wei Li Hsiang-Ru Lin Chang-Syun Yang Yi-Cheng Chu Tzong-Huei Lee Jih-Jung Chen |
| author_facet | Wei-Cheng Chen Shih-Wei Wang Cai-Wei Li Hsiang-Ru Lin Chang-Syun Yang Yi-Cheng Chu Tzong-Huei Lee Jih-Jung Chen |
| author_sort | Wei-Cheng Chen |
| collection | DOAJ |
| description | <i>Portulaca oleracea</i> is a well-known species for traditional medicine and food homology in Taiwan. In traditional medicine, <i>P. oleracea</i> is also used to treat gastrointestinal disorders, liver inflammation, fever, severe inflammation, and headaches. We investigated antioxidant, anti-tyrosinase, and anti-α-glucosidase activities of various solvent extracts and major bioactive components from <i>P. oleracea</i>. Ethanol and acetone extracts showed potent DPPH, ABTS, and hydroxyl radical scavenging activities. Chloroform and <i>n</i>-hexane extracts displayed significant superoxide radical scavenging activity. Furthermore, ethyl acetate and acetone extracts of <i>P. oleracea</i> showed potent anti-tyrosinase and anti-α-glucosidase activities. Examined and compared to the various solvent extracts for their chemical compositions using HPLC analysis, we isolated seven major compounds and analyzed their antioxidant, anti-tyrosinase, and anti-α-glucosidase activities. Seven active compounds of <i>P. oleracea</i>, especially quercetin, rosmarinic acid, and kaempferol, exhibited obvious antioxidant, anti-tyrosinase, and anti-α-glucosidase activities. The molecular docking model and the hydrophilic interactive mode of tyrosinase and α-glucosidase revealed that active compounds might have a higher antagonistic effect than commonly inhibitors. Our result shows that the active solvent extracts and their components of <i>P. oleracea</i> have the potential as natural antioxidants, tyrosinase and α-glucosidase inhibitors. Our results suggest that the active solvent extracts of <i>P. oleracea</i> and their components have potential as natural antioxidants, tyrosinase and α-glucosidase inhibitors. |
| first_indexed | 2024-03-09T22:43:55Z |
| format | Article |
| id | doaj.art-d6bfb162d7db4bdd8c3471a25396154e |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T22:43:55Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-d6bfb162d7db4bdd8c3471a25396154e2023-11-23T18:33:11ZengMDPI AGAntioxidants2076-39212022-02-0111239810.3390/antiox11020398Comparison of Various Solvent Extracts and Major Bioactive Components from <i>Portulaca oleracea</i> for Antioxidant, Anti-Tyrosinase, and Anti-α-Glucosidase ActivitiesWei-Cheng Chen0Shih-Wei Wang1Cai-Wei Li2Hsiang-Ru Lin3Chang-Syun Yang4Yi-Cheng Chu5Tzong-Huei Lee6Jih-Jung Chen7Department of Medicine, MacKay Medical College, New Taipei City 252, TaiwanDepartment of Medicine, MacKay Medical College, New Taipei City 252, TaiwanInstitute of Traditional Medicine, National Yang Ming Chiao Tung University, Taipei 112, TaiwanDepartment of Chemistry, College of Science, National Kaohsiung Normal University, Kaohsiung 824, TaiwanDepartment of Pharmacy, School of Pharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei 112, TaiwanInstitute of Traditional Medicine, National Yang Ming Chiao Tung University, Taipei 112, TaiwanInstitute of Fisheries Science, National Taiwan University, Taipei 106, TaiwanDepartment of Pharmacy, School of Pharmaceutical Sciences, National Yang Ming Chiao Tung University, Taipei 112, Taiwan<i>Portulaca oleracea</i> is a well-known species for traditional medicine and food homology in Taiwan. In traditional medicine, <i>P. oleracea</i> is also used to treat gastrointestinal disorders, liver inflammation, fever, severe inflammation, and headaches. We investigated antioxidant, anti-tyrosinase, and anti-α-glucosidase activities of various solvent extracts and major bioactive components from <i>P. oleracea</i>. Ethanol and acetone extracts showed potent DPPH, ABTS, and hydroxyl radical scavenging activities. Chloroform and <i>n</i>-hexane extracts displayed significant superoxide radical scavenging activity. Furthermore, ethyl acetate and acetone extracts of <i>P. oleracea</i> showed potent anti-tyrosinase and anti-α-glucosidase activities. Examined and compared to the various solvent extracts for their chemical compositions using HPLC analysis, we isolated seven major compounds and analyzed their antioxidant, anti-tyrosinase, and anti-α-glucosidase activities. Seven active compounds of <i>P. oleracea</i>, especially quercetin, rosmarinic acid, and kaempferol, exhibited obvious antioxidant, anti-tyrosinase, and anti-α-glucosidase activities. The molecular docking model and the hydrophilic interactive mode of tyrosinase and α-glucosidase revealed that active compounds might have a higher antagonistic effect than commonly inhibitors. Our result shows that the active solvent extracts and their components of <i>P. oleracea</i> have the potential as natural antioxidants, tyrosinase and α-glucosidase inhibitors. Our results suggest that the active solvent extracts of <i>P. oleracea</i> and their components have potential as natural antioxidants, tyrosinase and α-glucosidase inhibitors.https://www.mdpi.com/2076-3921/11/2/398<i>Portulaca oleracea</i>various solvent extractsmajor bioactive componentsantioxidant activityanti-α-glucosidase activityanti-tyrosinase activity |
| spellingShingle | Wei-Cheng Chen Shih-Wei Wang Cai-Wei Li Hsiang-Ru Lin Chang-Syun Yang Yi-Cheng Chu Tzong-Huei Lee Jih-Jung Chen Comparison of Various Solvent Extracts and Major Bioactive Components from <i>Portulaca oleracea</i> for Antioxidant, Anti-Tyrosinase, and Anti-α-Glucosidase Activities Antioxidants <i>Portulaca oleracea</i> various solvent extracts major bioactive components antioxidant activity anti-α-glucosidase activity anti-tyrosinase activity |
| title | Comparison of Various Solvent Extracts and Major Bioactive Components from <i>Portulaca oleracea</i> for Antioxidant, Anti-Tyrosinase, and Anti-α-Glucosidase Activities |
| title_full | Comparison of Various Solvent Extracts and Major Bioactive Components from <i>Portulaca oleracea</i> for Antioxidant, Anti-Tyrosinase, and Anti-α-Glucosidase Activities |
| title_fullStr | Comparison of Various Solvent Extracts and Major Bioactive Components from <i>Portulaca oleracea</i> for Antioxidant, Anti-Tyrosinase, and Anti-α-Glucosidase Activities |
| title_full_unstemmed | Comparison of Various Solvent Extracts and Major Bioactive Components from <i>Portulaca oleracea</i> for Antioxidant, Anti-Tyrosinase, and Anti-α-Glucosidase Activities |
| title_short | Comparison of Various Solvent Extracts and Major Bioactive Components from <i>Portulaca oleracea</i> for Antioxidant, Anti-Tyrosinase, and Anti-α-Glucosidase Activities |
| title_sort | comparison of various solvent extracts and major bioactive components from i portulaca oleracea i for antioxidant anti tyrosinase and anti α glucosidase activities |
| topic | <i>Portulaca oleracea</i> various solvent extracts major bioactive components antioxidant activity anti-α-glucosidase activity anti-tyrosinase activity |
| url | https://www.mdpi.com/2076-3921/11/2/398 |
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