Nosocomial and community infections due to class A extended-spectrum β-lactamase (ESBLA)-producing Escherichia coli and Klebsiella spp. in southern Brazil
Objectives: To determine the prevalence of class A extended spectrum β-lactamases (ESBL)-producing Escherichia coli and Klebsiella spp., and to investigate clonality among ESBL-producing isolates of nosocomial and community infections. Methods: The study involved 354 nosocomial infections samples an...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2011-03-01
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| Series: | Brazilian Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867011701593 |
| _version_ | 1819226631460356096 |
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| author | Claudia Wollheim Ivani Maria F. Guerra Vania D. Conte Sheila P. Hoffman Fernando J. Schreiner Ana Paula L. Delamare Afonso L. Barth Sérgio Echeverrigaray Sérgio Olavo P. da Costa |
| author_facet | Claudia Wollheim Ivani Maria F. Guerra Vania D. Conte Sheila P. Hoffman Fernando J. Schreiner Ana Paula L. Delamare Afonso L. Barth Sérgio Echeverrigaray Sérgio Olavo P. da Costa |
| author_sort | Claudia Wollheim |
| collection | DOAJ |
| description | Objectives: To determine the prevalence of class A extended spectrum β-lactamases (ESBL)-producing Escherichia coli and Klebsiella spp., and to investigate clonality among ESBL-producing isolates of nosocomial and community infections. Methods: The study involved 354 nosocomial infections samples and 992 community infections samples, obtained between 2003 and 2006 at Caxias do Sul, RS. The detection of ESBL was performed by the disk-diffusion test. Presence of blaCTX-M, blaSHV and blaTEM β-lactamase genes was evaluated by PCR, and genomic typing was determined by pulsed-field gel electrophoresis analysis. Results: Higher frequency of ESBL-producing isolates were detected among nosocomial samples of E. coli (6.7%) and Klebsiella (43.7%), than those obtained from community infections (0.4% and 2.6%). blaTEM and blaCTX were the most prevalent ESBL gene families in both E. coli and Klebsiella isolates. Different pulsotypes were obtained among ESBL-producing E. coli and 11 clones for Klebsiella spp., which occurred over the years and in different hospital wards. Among ESBL-producing K. pneumoniae, 74.3% transferred ESBL genes by conjugation and exhibited concomitant decreased aminoglycosides susceptibility. Conclusion: ESBL-producing E. coli, and especially K. pneumoniae are essentially a nosocomial problem, and their dissemination to the community is relatively limited. The great genetic variability observed among ESBL-producing bacteria indicates polyclonal spread and high transference of ESBL genes between bacteria in the hospital environment. This information is of paramount importance for nosocomial infection control. Keywords: β-lactamases, polymerase chain reaction, bacterial typing techniques |
| first_indexed | 2024-12-23T10:28:34Z |
| format | Article |
| id | doaj.art-d6c0fac07ae440aabe69f0c58172e3e7 |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-12-23T10:28:34Z |
| publishDate | 2011-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-d6c0fac07ae440aabe69f0c58172e3e72022-12-21T17:50:29ZengElsevierBrazilian Journal of Infectious Diseases1413-86702011-03-01152138143Nosocomial and community infections due to class A extended-spectrum β-lactamase (ESBLA)-producing Escherichia coli and Klebsiella spp. in southern BrazilClaudia Wollheim0Ivani Maria F. Guerra1Vania D. Conte2Sheila P. Hoffman3Fernando J. Schreiner4Ana Paula L. Delamare5Afonso L. Barth6Sérgio Echeverrigaray7Sérgio Olavo P. da Costa8PhD, Medical, Microbiology Laboratory, Universidade de Caxias do Sul, RS, Brazil; Correspondence to: Laboratório de Microbiologia Médica Humana Universidade de Caxias do Sul Rua Francisco Getúlio Vargas, 1130, Petrópolis, Caxias do Sul, RS, Brazil 95001-970 Phone: +55 54 3218-2548 Fax: +55 54 3218-2041.MS; Medical Microbiology, Laboratory, Universidade de Caxias do Sul, RS, BrazilPharmacist; Medical, Microbiology Laboratory, Universidade de Caxias do Sul, RS, BrazilPharmacist; Medical, Microbiology Laboratory, Universidade de Caxias do Sul, RS, BrazilPhD, Medical, Microbiology Laboratory, Universidade de Caxias do Sul, RS, BrazilPhD,Instituto de, Biotecnologia, Universidade de Caxias do Sul, RS, BrazilPhD, Hospital de Clínicas, de Porto Alegre, RS, BrazilPhD, Instituto de, Biotecnologia, Universidade de Caxias do Sul, RS, BrazilPhD, Instituto de, Biotecnologia, Universidade de Caxias do Sul, RS, BrazilObjectives: To determine the prevalence of class A extended spectrum β-lactamases (ESBL)-producing Escherichia coli and Klebsiella spp., and to investigate clonality among ESBL-producing isolates of nosocomial and community infections. Methods: The study involved 354 nosocomial infections samples and 992 community infections samples, obtained between 2003 and 2006 at Caxias do Sul, RS. The detection of ESBL was performed by the disk-diffusion test. Presence of blaCTX-M, blaSHV and blaTEM β-lactamase genes was evaluated by PCR, and genomic typing was determined by pulsed-field gel electrophoresis analysis. Results: Higher frequency of ESBL-producing isolates were detected among nosocomial samples of E. coli (6.7%) and Klebsiella (43.7%), than those obtained from community infections (0.4% and 2.6%). blaTEM and blaCTX were the most prevalent ESBL gene families in both E. coli and Klebsiella isolates. Different pulsotypes were obtained among ESBL-producing E. coli and 11 clones for Klebsiella spp., which occurred over the years and in different hospital wards. Among ESBL-producing K. pneumoniae, 74.3% transferred ESBL genes by conjugation and exhibited concomitant decreased aminoglycosides susceptibility. Conclusion: ESBL-producing E. coli, and especially K. pneumoniae are essentially a nosocomial problem, and their dissemination to the community is relatively limited. The great genetic variability observed among ESBL-producing bacteria indicates polyclonal spread and high transference of ESBL genes between bacteria in the hospital environment. This information is of paramount importance for nosocomial infection control. Keywords: β-lactamases, polymerase chain reaction, bacterial typing techniqueshttp://www.sciencedirect.com/science/article/pii/S1413867011701593 |
| spellingShingle | Claudia Wollheim Ivani Maria F. Guerra Vania D. Conte Sheila P. Hoffman Fernando J. Schreiner Ana Paula L. Delamare Afonso L. Barth Sérgio Echeverrigaray Sérgio Olavo P. da Costa Nosocomial and community infections due to class A extended-spectrum β-lactamase (ESBLA)-producing Escherichia coli and Klebsiella spp. in southern Brazil Brazilian Journal of Infectious Diseases |
| title | Nosocomial and community infections due to class A extended-spectrum β-lactamase (ESBLA)-producing Escherichia coli and Klebsiella spp. in southern Brazil |
| title_full | Nosocomial and community infections due to class A extended-spectrum β-lactamase (ESBLA)-producing Escherichia coli and Klebsiella spp. in southern Brazil |
| title_fullStr | Nosocomial and community infections due to class A extended-spectrum β-lactamase (ESBLA)-producing Escherichia coli and Klebsiella spp. in southern Brazil |
| title_full_unstemmed | Nosocomial and community infections due to class A extended-spectrum β-lactamase (ESBLA)-producing Escherichia coli and Klebsiella spp. in southern Brazil |
| title_short | Nosocomial and community infections due to class A extended-spectrum β-lactamase (ESBLA)-producing Escherichia coli and Klebsiella spp. in southern Brazil |
| title_sort | nosocomial and community infections due to class a extended spectrum β lactamase esbla producing escherichia coli and klebsiella spp in southern brazil |
| url | http://www.sciencedirect.com/science/article/pii/S1413867011701593 |
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