Survey on the contamination rate and determination of antibiotic resistance of Staphylococcus aureus, Escherichia coli and Salmonella strains isolated from traditional cheeses distributed in Mahabad, Iran
Background and Objective: Traditional cheese has desirable organoleptic characteristics for Iranian consumers. Escherichia coli, Staphylococcus aureus and Salmonella are some of the most important microbial agents in food poisoning and gastrointestinal infections, which can contaminate this product...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Tehran University of Medical Sciences
2019-03-01
|
| Series: | سلامت و محیط |
| Subjects: | |
| Online Access: | http://ijhe.tums.ac.ir/article-1-6183-en.html |
| _version_ | 1818670306178367488 |
|---|---|
| author | S Hamzeh Pour S Vaziri E Molaee Aghaee |
| author_facet | S Hamzeh Pour S Vaziri E Molaee Aghaee |
| author_sort | S Hamzeh Pour |
| collection | DOAJ |
| description | Background and Objective: Traditional cheese has desirable organoleptic characteristics for Iranian consumers. Escherichia coli, Staphylococcus aureus and Salmonella are some of the most important microbial agents in food poisoning and gastrointestinal infections, which can contaminate this product and endanger the health of consumers. The aim of this study was to survey the contamination rate and determination of antibiotic resistance of Staphylococcus aureus, Escherichia coli and Salmonella strains isolated from traditional cheeses distributed in Mahabad, Iran.
Materials and Methods: In a cross-sectional study, 100 samples of traditional cheese from the production and distribution centers in Mahabad city were randomly selected in 2016 to determine the bacterial contamination in accordance to national standard guidelines for the presence of Staphylococcus aureus, E.coli and Salmonella spp. Anti-biograms test was conducted using a standard disc diffusion method through the measurement of the inhibitory zone diameter on the Muller Hinton agar.
Results: Among the 100 samples, 54, 45 and 0 samples were positive for Escherichia coli, Staphylococcus aureus and Salmonella, respectively. All E.coli (100%) were found to be resistant to Amoxicillin, while Staphylococcus aureus isolates (100%) were resistant to Amoxicillin-Clavulanic acid and Amoxicillin. However, 66.6% isolates of E.coli were susceptible to Ceftriaxone and 97.7% isolates of Staphylococcus aureus were susceptible to Trimethoprim – Sulfamethoxazole.
Conclusion: The microbial quality of traditional cheeses distributed in Mahabad city was not evaluated as appropriate and the values did not meet the national standards. Thus, further monitoring and control are needed. |
| first_indexed | 2024-12-17T07:06:00Z |
| format | Article |
| id | doaj.art-d6c1c8d686d64e02a9935f00e807aff9 |
| institution | Directory Open Access Journal |
| issn | 2008-2029 2008-3718 |
| language | fas |
| last_indexed | 2024-12-17T07:06:00Z |
| publishDate | 2019-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | سلامت و محیط |
| spelling | doaj.art-d6c1c8d686d64e02a9935f00e807aff92022-12-21T21:59:11ZfasTehran University of Medical Sciencesسلامت و محیط2008-20292008-37182019-03-01114465476Survey on the contamination rate and determination of antibiotic resistance of Staphylococcus aureus, Escherichia coli and Salmonella strains isolated from traditional cheeses distributed in Mahabad, IranS Hamzeh Pour0S Vaziri1E Molaee Aghaee2 Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Department of Biology, Payam Nour University of Tehran, Tehran, Iran Department of Environmental Health, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Background and Objective: Traditional cheese has desirable organoleptic characteristics for Iranian consumers. Escherichia coli, Staphylococcus aureus and Salmonella are some of the most important microbial agents in food poisoning and gastrointestinal infections, which can contaminate this product and endanger the health of consumers. The aim of this study was to survey the contamination rate and determination of antibiotic resistance of Staphylococcus aureus, Escherichia coli and Salmonella strains isolated from traditional cheeses distributed in Mahabad, Iran. Materials and Methods: In a cross-sectional study, 100 samples of traditional cheese from the production and distribution centers in Mahabad city were randomly selected in 2016 to determine the bacterial contamination in accordance to national standard guidelines for the presence of Staphylococcus aureus, E.coli and Salmonella spp. Anti-biograms test was conducted using a standard disc diffusion method through the measurement of the inhibitory zone diameter on the Muller Hinton agar. Results: Among the 100 samples, 54, 45 and 0 samples were positive for Escherichia coli, Staphylococcus aureus and Salmonella, respectively. All E.coli (100%) were found to be resistant to Amoxicillin, while Staphylococcus aureus isolates (100%) were resistant to Amoxicillin-Clavulanic acid and Amoxicillin. However, 66.6% isolates of E.coli were susceptible to Ceftriaxone and 97.7% isolates of Staphylococcus aureus were susceptible to Trimethoprim – Sulfamethoxazole. Conclusion: The microbial quality of traditional cheeses distributed in Mahabad city was not evaluated as appropriate and the values did not meet the national standards. Thus, further monitoring and control are needed.http://ijhe.tums.ac.ir/article-1-6183-en.htmltraditional cheeseantibiotic resistanceescherichia colistaphylococcus aureussalmonella |
| spellingShingle | S Hamzeh Pour S Vaziri E Molaee Aghaee Survey on the contamination rate and determination of antibiotic resistance of Staphylococcus aureus, Escherichia coli and Salmonella strains isolated from traditional cheeses distributed in Mahabad, Iran سلامت و محیط traditional cheese antibiotic resistance escherichia coli staphylococcus aureus salmonella |
| title | Survey on the contamination rate and determination of antibiotic resistance of Staphylococcus aureus, Escherichia coli and Salmonella strains isolated from traditional cheeses distributed in Mahabad, Iran |
| title_full | Survey on the contamination rate and determination of antibiotic resistance of Staphylococcus aureus, Escherichia coli and Salmonella strains isolated from traditional cheeses distributed in Mahabad, Iran |
| title_fullStr | Survey on the contamination rate and determination of antibiotic resistance of Staphylococcus aureus, Escherichia coli and Salmonella strains isolated from traditional cheeses distributed in Mahabad, Iran |
| title_full_unstemmed | Survey on the contamination rate and determination of antibiotic resistance of Staphylococcus aureus, Escherichia coli and Salmonella strains isolated from traditional cheeses distributed in Mahabad, Iran |
| title_short | Survey on the contamination rate and determination of antibiotic resistance of Staphylococcus aureus, Escherichia coli and Salmonella strains isolated from traditional cheeses distributed in Mahabad, Iran |
| title_sort | survey on the contamination rate and determination of antibiotic resistance of staphylococcus aureus escherichia coli and salmonella strains isolated from traditional cheeses distributed in mahabad iran |
| topic | traditional cheese antibiotic resistance escherichia coli staphylococcus aureus salmonella |
| url | http://ijhe.tums.ac.ir/article-1-6183-en.html |
| work_keys_str_mv | AT shamzehpour surveyonthecontaminationrateanddeterminationofantibioticresistanceofstaphylococcusaureusescherichiacoliandsalmonellastrainsisolatedfromtraditionalcheesesdistributedinmahabadiran AT svaziri surveyonthecontaminationrateanddeterminationofantibioticresistanceofstaphylococcusaureusescherichiacoliandsalmonellastrainsisolatedfromtraditionalcheesesdistributedinmahabadiran AT emolaeeaghaee surveyonthecontaminationrateanddeterminationofantibioticresistanceofstaphylococcusaureusescherichiacoliandsalmonellastrainsisolatedfromtraditionalcheesesdistributedinmahabadiran |