ELISA based assays to measure adenosine deaminases concentration in serum and saliva for the diagnosis of ADA2 deficiency and cancer
Adenosine deaminases (ADAs) are enzymes of purine metabolism converting adenosine to inosine. There are two types of ADAs in humans ADA1 and ADA2. While both ADA1 and ADA2 share the same substrate, they differ in expression, cellular localization, and catalytic properties. The genetic deficiency of...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-07-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.928438/full |
| _version_ | 1811311943131070464 |
|---|---|
| author | Wenwen Luo Liang Dong Fenghong Chen Wenbin Lei Liya He Qing Zhou Qing Zhou Thierry Lamy Andrey V. Zavialov Andrey V. Zavialov |
| author_facet | Wenwen Luo Liang Dong Fenghong Chen Wenbin Lei Liya He Qing Zhou Qing Zhou Thierry Lamy Andrey V. Zavialov Andrey V. Zavialov |
| author_sort | Wenwen Luo |
| collection | DOAJ |
| description | Adenosine deaminases (ADAs) are enzymes of purine metabolism converting adenosine to inosine. There are two types of ADAs in humans ADA1 and ADA2. While both ADA1 and ADA2 share the same substrate, they differ in expression, cellular localization, and catalytic properties. The genetic deficiency of ADA1 results in severe combined immunodeficiency (SCID), while lack in ADA2 (DADA2) results in multiple phenotypes ranging from systemic inflammation to vascular pathology. Clinical studies have shown that the levels of ADAs in biological fluids are altered in pathophysiological conditions, suggesting that ADA activity could be a convenient marker for the diagnosis of immune diseases and cancer. Here, we describe sensitive and straightforward ELISA assays to measure ADA1 and ADA2 concentrations in biological fluids. Analysis of the serum and saliva samples from the healthy controls and DADA2 patients revealed that ADA2 enzyme concentration is significantly lower in patients than in healthy controls. In contrast, the concentration of ADA2 increases in the serum of patients with large granular leukocyte leukemia (LGLL) and patients’ saliva with head and neck cancer. Thus, this simple, non-invasive method allows for distinguishing healthy controls from the affected patient. It can be implemented in screening and diagnosis of DADA2 and follow up the treatment of LGLL and several types of head and neck cancer. |
| first_indexed | 2024-04-13T10:27:50Z |
| format | Article |
| id | doaj.art-d6c2322f2e7c4712a903e631ee77b29d |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-13T10:27:50Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-d6c2322f2e7c4712a903e631ee77b29d2022-12-22T02:50:17ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-07-011310.3389/fimmu.2022.928438928438ELISA based assays to measure adenosine deaminases concentration in serum and saliva for the diagnosis of ADA2 deficiency and cancerWenwen Luo0Liang Dong1Fenghong Chen2Wenbin Lei3Liya He4Qing Zhou5Qing Zhou6Thierry Lamy7Andrey V. Zavialov8Andrey V. Zavialov9Guangzhou Institute of Pediatrics, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, ChinaGuangzhou Institute of Pediatrics, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, ChinaOtorhinolaryngology Hospital, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaOtorhinolaryngology Hospital, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaGuangzhou Institute of Pediatrics, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, ChinaLife Sciences Institute, Zhejiang University, Hangzhou, ChinaLiangzhu Laboratory, Zhejiang University Medical Center, Hangzhou, ChinaDepartment of Hematology, Pontchaillou University Hospital, Rennes, FranceGuangzhou Institute of Pediatrics, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, ChinaTurku Centre for Biotechnology, University of Turku, Turku, FinlandAdenosine deaminases (ADAs) are enzymes of purine metabolism converting adenosine to inosine. There are two types of ADAs in humans ADA1 and ADA2. While both ADA1 and ADA2 share the same substrate, they differ in expression, cellular localization, and catalytic properties. The genetic deficiency of ADA1 results in severe combined immunodeficiency (SCID), while lack in ADA2 (DADA2) results in multiple phenotypes ranging from systemic inflammation to vascular pathology. Clinical studies have shown that the levels of ADAs in biological fluids are altered in pathophysiological conditions, suggesting that ADA activity could be a convenient marker for the diagnosis of immune diseases and cancer. Here, we describe sensitive and straightforward ELISA assays to measure ADA1 and ADA2 concentrations in biological fluids. Analysis of the serum and saliva samples from the healthy controls and DADA2 patients revealed that ADA2 enzyme concentration is significantly lower in patients than in healthy controls. In contrast, the concentration of ADA2 increases in the serum of patients with large granular leukocyte leukemia (LGLL) and patients’ saliva with head and neck cancer. Thus, this simple, non-invasive method allows for distinguishing healthy controls from the affected patient. It can be implemented in screening and diagnosis of DADA2 and follow up the treatment of LGLL and several types of head and neck cancer.https://www.frontiersin.org/articles/10.3389/fimmu.2022.928438/fulladenosine deaminase 2ADA2 deficiencyhead and neck cancerlarge granular leukocyte leukemiaadenosine deaminase |
| spellingShingle | Wenwen Luo Liang Dong Fenghong Chen Wenbin Lei Liya He Qing Zhou Qing Zhou Thierry Lamy Andrey V. Zavialov Andrey V. Zavialov ELISA based assays to measure adenosine deaminases concentration in serum and saliva for the diagnosis of ADA2 deficiency and cancer Frontiers in Immunology adenosine deaminase 2 ADA2 deficiency head and neck cancer large granular leukocyte leukemia adenosine deaminase |
| title | ELISA based assays to measure adenosine deaminases concentration in serum and saliva for the diagnosis of ADA2 deficiency and cancer |
| title_full | ELISA based assays to measure adenosine deaminases concentration in serum and saliva for the diagnosis of ADA2 deficiency and cancer |
| title_fullStr | ELISA based assays to measure adenosine deaminases concentration in serum and saliva for the diagnosis of ADA2 deficiency and cancer |
| title_full_unstemmed | ELISA based assays to measure adenosine deaminases concentration in serum and saliva for the diagnosis of ADA2 deficiency and cancer |
| title_short | ELISA based assays to measure adenosine deaminases concentration in serum and saliva for the diagnosis of ADA2 deficiency and cancer |
| title_sort | elisa based assays to measure adenosine deaminases concentration in serum and saliva for the diagnosis of ada2 deficiency and cancer |
| topic | adenosine deaminase 2 ADA2 deficiency head and neck cancer large granular leukocyte leukemia adenosine deaminase |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.928438/full |
| work_keys_str_mv | AT wenwenluo elisabasedassaystomeasureadenosinedeaminasesconcentrationinserumandsalivaforthediagnosisofada2deficiencyandcancer AT liangdong elisabasedassaystomeasureadenosinedeaminasesconcentrationinserumandsalivaforthediagnosisofada2deficiencyandcancer AT fenghongchen elisabasedassaystomeasureadenosinedeaminasesconcentrationinserumandsalivaforthediagnosisofada2deficiencyandcancer AT wenbinlei elisabasedassaystomeasureadenosinedeaminasesconcentrationinserumandsalivaforthediagnosisofada2deficiencyandcancer AT liyahe elisabasedassaystomeasureadenosinedeaminasesconcentrationinserumandsalivaforthediagnosisofada2deficiencyandcancer AT qingzhou elisabasedassaystomeasureadenosinedeaminasesconcentrationinserumandsalivaforthediagnosisofada2deficiencyandcancer AT qingzhou elisabasedassaystomeasureadenosinedeaminasesconcentrationinserumandsalivaforthediagnosisofada2deficiencyandcancer AT thierrylamy elisabasedassaystomeasureadenosinedeaminasesconcentrationinserumandsalivaforthediagnosisofada2deficiencyandcancer AT andreyvzavialov elisabasedassaystomeasureadenosinedeaminasesconcentrationinserumandsalivaforthediagnosisofada2deficiencyandcancer AT andreyvzavialov elisabasedassaystomeasureadenosinedeaminasesconcentrationinserumandsalivaforthediagnosisofada2deficiencyandcancer |