MOLECULAR CYTOGENETIC CHARACTERIZATION OF SORGHUM CHROMOSOMES IN SACCHARUM BACKGROUND AND VICE VERSA USING GENOMIC IN SITU HYBRIDIZATION
Two intergeneric hybrids of sugarcane, Co 86032 (2n=112) x Sorghum (2n=20) and Sorghum (2n=20) x S. officinarum (2n= 112), were subjected to classical as well as molecular cytological analysis. The hybridity of these clones has been confirmed by using SSR markers and chloroplast gene. During genomic...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Society for Sugarcane Research and Development
2019-03-01
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| Series: | Journal of Sugarcane Research |
| Online Access: | https://epubs.icar.org.in/index.php/JSR/article/view/89055 |
| _version_ | 1828820887601676288 |
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| author | V P Sobhakumari N V Nair K Mohanraj Maya Lekshmi |
| author_facet | V P Sobhakumari N V Nair K Mohanraj Maya Lekshmi |
| author_sort | V P Sobhakumari |
| collection | DOAJ |
| description | Two intergeneric hybrids of sugarcane, Co 86032 (2n=112) x Sorghum (2n=20) and Sorghum (2n=20) x S. officinarum (2n= 112), were subjected to classical as well as molecular cytological analysis. The hybridity of these clones has been confirmed by using SSR markers and chloroplast gene. During genomic in situ hybridization (GISH) the genome composition of the hybrids studied and it has revealed that it follows n+n chromosome segregation. Introgression of 10 Sorghum chromosomes in both the hybrids was observed while using Sorghum genomic DNA as the labeled probe. We report for the first time the chromosome composition and condensation behavior of Sorghum chromosomes in hybrids involving Saccharum and Sorghum. |
| first_indexed | 2024-12-12T12:43:24Z |
| format | Article |
| id | doaj.art-d6c23ae0e7284cb799af023e384c429d |
| institution | Directory Open Access Journal |
| issn | 2249-927X 2582-4767 |
| language | English |
| last_indexed | 2024-12-12T12:43:24Z |
| publishDate | 2019-03-01 |
| publisher | Society for Sugarcane Research and Development |
| record_format | Article |
| series | Journal of Sugarcane Research |
| spelling | doaj.art-d6c23ae0e7284cb799af023e384c429d2022-12-22T00:24:10ZengSociety for Sugarcane Research and DevelopmentJournal of Sugarcane Research2249-927X2582-47672019-03-0181MOLECULAR CYTOGENETIC CHARACTERIZATION OF SORGHUM CHROMOSOMES IN SACCHARUM BACKGROUND AND VICE VERSA USING GENOMIC IN SITU HYBRIDIZATIONV P Sobhakumari0N V Nair1K Mohanraj2Maya Lekshmi3ICAR-Sugarcane Breeding Institute, Coimbatore - 641007ICAR-Sugarcane Breeding Institute, Coimbatore - 641007ICAR-Sugarcane Breeding Institute, Coimbatore - 641007ICAR-Sugarcane Breeding Institute, Coimbatore - 641007Two intergeneric hybrids of sugarcane, Co 86032 (2n=112) x Sorghum (2n=20) and Sorghum (2n=20) x S. officinarum (2n= 112), were subjected to classical as well as molecular cytological analysis. The hybridity of these clones has been confirmed by using SSR markers and chloroplast gene. During genomic in situ hybridization (GISH) the genome composition of the hybrids studied and it has revealed that it follows n+n chromosome segregation. Introgression of 10 Sorghum chromosomes in both the hybrids was observed while using Sorghum genomic DNA as the labeled probe. We report for the first time the chromosome composition and condensation behavior of Sorghum chromosomes in hybrids involving Saccharum and Sorghum.https://epubs.icar.org.in/index.php/JSR/article/view/89055 |
| spellingShingle | V P Sobhakumari N V Nair K Mohanraj Maya Lekshmi MOLECULAR CYTOGENETIC CHARACTERIZATION OF SORGHUM CHROMOSOMES IN SACCHARUM BACKGROUND AND VICE VERSA USING GENOMIC IN SITU HYBRIDIZATION Journal of Sugarcane Research |
| title | MOLECULAR CYTOGENETIC CHARACTERIZATION OF SORGHUM CHROMOSOMES IN SACCHARUM BACKGROUND AND VICE VERSA USING GENOMIC IN SITU HYBRIDIZATION |
| title_full | MOLECULAR CYTOGENETIC CHARACTERIZATION OF SORGHUM CHROMOSOMES IN SACCHARUM BACKGROUND AND VICE VERSA USING GENOMIC IN SITU HYBRIDIZATION |
| title_fullStr | MOLECULAR CYTOGENETIC CHARACTERIZATION OF SORGHUM CHROMOSOMES IN SACCHARUM BACKGROUND AND VICE VERSA USING GENOMIC IN SITU HYBRIDIZATION |
| title_full_unstemmed | MOLECULAR CYTOGENETIC CHARACTERIZATION OF SORGHUM CHROMOSOMES IN SACCHARUM BACKGROUND AND VICE VERSA USING GENOMIC IN SITU HYBRIDIZATION |
| title_short | MOLECULAR CYTOGENETIC CHARACTERIZATION OF SORGHUM CHROMOSOMES IN SACCHARUM BACKGROUND AND VICE VERSA USING GENOMIC IN SITU HYBRIDIZATION |
| title_sort | molecular cytogenetic characterization of sorghum chromosomes in saccharum background and vice versa using genomic in situ hybridization |
| url | https://epubs.icar.org.in/index.php/JSR/article/view/89055 |
| work_keys_str_mv | AT vpsobhakumari molecularcytogeneticcharacterizationofsorghumchromosomesinsaccharumbackgroundandviceversausinggenomicinsituhybridization AT nvnair molecularcytogeneticcharacterizationofsorghumchromosomesinsaccharumbackgroundandviceversausinggenomicinsituhybridization AT kmohanraj molecularcytogeneticcharacterizationofsorghumchromosomesinsaccharumbackgroundandviceversausinggenomicinsituhybridization AT mayalekshmi molecularcytogeneticcharacterizationofsorghumchromosomesinsaccharumbackgroundandviceversausinggenomicinsituhybridization |