A Fast Scoring of Human Primary Respiratory Epithelia Grown at Air–Liquid Interface (ALI) to Assess Epithelial Morphology in Research and Personalized Medicine Settings
Background: In recent years, increasingly complex ALI protocols involving specialized, albeit laboratory-specific media have been established, while at the same time, many studies compile the data of only a few ALI donors in spite of site-, protocol- and donor-specific differentiation. Methods: We d...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Journal of Personalized Medicine |
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| Online Access: | https://www.mdpi.com/2075-4426/14/1/109 |
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| author | Christopher T. Lutsch Longhua Feng Ana Gómez Hohn Lennart Brandt Stephanie Tamm Sabina Janciauskiene Frauke Stanke Danny Jonigk Anna-Maria Dittrich Peter Braubach |
| author_facet | Christopher T. Lutsch Longhua Feng Ana Gómez Hohn Lennart Brandt Stephanie Tamm Sabina Janciauskiene Frauke Stanke Danny Jonigk Anna-Maria Dittrich Peter Braubach |
| author_sort | Christopher T. Lutsch |
| collection | DOAJ |
| description | Background: In recent years, increasingly complex ALI protocols involving specialized, albeit laboratory-specific media have been established, while at the same time, many studies compile the data of only a few ALI donors in spite of site-, protocol- and donor-specific differentiation. Methods: We describe a simple morphology scoring protocol using histology material derived from epithelia grown on ALI inserts in parallel to other, more complex readouts. Results: Among more than 100 ALI inserts derived from different donors, significant differences in layer score (<i>p</i> = 0.001) and goblet cell score (<i>p</i> = 0.002) were observed when ALI epithelia derived from explanted lung material were compared to trachea-derived ALI cultures. Cortisol withdrawal for the final 2 days of ALI cultures influenced goblet cell density (<i>p</i> = 0.001). Conclusions: While the histology score provides less resolution than FACS- or OMICs- based single cell analyses, the use of a subportion of the ALI epithelia grown on inserts makes it feasible to combine morphology assessment and other readouts of the same insert. This allows us to control for basic ALI morphology in research and personalized medicine settings in order to assess and, if desired, control for the impact of ALI culture protocols, site- and donor-specific influences on outcome of studies of ALI-derived epithelia. |
| first_indexed | 2024-03-08T10:44:44Z |
| format | Article |
| id | doaj.art-d6c27578b52941d78411c16e98d93528 |
| institution | Directory Open Access Journal |
| issn | 2075-4426 |
| language | English |
| last_indexed | 2024-03-08T10:44:44Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Personalized Medicine |
| spelling | doaj.art-d6c27578b52941d78411c16e98d935282024-01-26T17:19:37ZengMDPI AGJournal of Personalized Medicine2075-44262024-01-0114110910.3390/jpm14010109A Fast Scoring of Human Primary Respiratory Epithelia Grown at Air–Liquid Interface (ALI) to Assess Epithelial Morphology in Research and Personalized Medicine SettingsChristopher T. Lutsch0Longhua Feng1Ana Gómez Hohn2Lennart Brandt3Stephanie Tamm4Sabina Janciauskiene5Frauke Stanke6Danny Jonigk7Anna-Maria Dittrich8Peter Braubach9Department of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625 Hannover, GermanyDepartment of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625 Hannover, GermanyInstitute for Pathology, Hannover Medical School, 30625 Hannover, GermanyInstitute for Pathology, Hannover Medical School, 30625 Hannover, GermanyDepartment of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625 Hannover, GermanyBiomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), German Center for Lung Research (DZL), 30625 Hannover, GermanyDepartment of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625 Hannover, GermanyBiomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), German Center for Lung Research (DZL), 30625 Hannover, GermanyDepartment of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625 Hannover, GermanyInstitute for Pathology, Hannover Medical School, 30625 Hannover, GermanyBackground: In recent years, increasingly complex ALI protocols involving specialized, albeit laboratory-specific media have been established, while at the same time, many studies compile the data of only a few ALI donors in spite of site-, protocol- and donor-specific differentiation. Methods: We describe a simple morphology scoring protocol using histology material derived from epithelia grown on ALI inserts in parallel to other, more complex readouts. Results: Among more than 100 ALI inserts derived from different donors, significant differences in layer score (<i>p</i> = 0.001) and goblet cell score (<i>p</i> = 0.002) were observed when ALI epithelia derived from explanted lung material were compared to trachea-derived ALI cultures. Cortisol withdrawal for the final 2 days of ALI cultures influenced goblet cell density (<i>p</i> = 0.001). Conclusions: While the histology score provides less resolution than FACS- or OMICs- based single cell analyses, the use of a subportion of the ALI epithelia grown on inserts makes it feasible to combine morphology assessment and other readouts of the same insert. This allows us to control for basic ALI morphology in research and personalized medicine settings in order to assess and, if desired, control for the impact of ALI culture protocols, site- and donor-specific influences on outcome of studies of ALI-derived epithelia.https://www.mdpi.com/2075-4426/14/1/109primary respiratory epitheliaair–liquid interface culturemorphology scoregoblet cells |
| spellingShingle | Christopher T. Lutsch Longhua Feng Ana Gómez Hohn Lennart Brandt Stephanie Tamm Sabina Janciauskiene Frauke Stanke Danny Jonigk Anna-Maria Dittrich Peter Braubach A Fast Scoring of Human Primary Respiratory Epithelia Grown at Air–Liquid Interface (ALI) to Assess Epithelial Morphology in Research and Personalized Medicine Settings Journal of Personalized Medicine primary respiratory epithelia air–liquid interface culture morphology score goblet cells |
| title | A Fast Scoring of Human Primary Respiratory Epithelia Grown at Air–Liquid Interface (ALI) to Assess Epithelial Morphology in Research and Personalized Medicine Settings |
| title_full | A Fast Scoring of Human Primary Respiratory Epithelia Grown at Air–Liquid Interface (ALI) to Assess Epithelial Morphology in Research and Personalized Medicine Settings |
| title_fullStr | A Fast Scoring of Human Primary Respiratory Epithelia Grown at Air–Liquid Interface (ALI) to Assess Epithelial Morphology in Research and Personalized Medicine Settings |
| title_full_unstemmed | A Fast Scoring of Human Primary Respiratory Epithelia Grown at Air–Liquid Interface (ALI) to Assess Epithelial Morphology in Research and Personalized Medicine Settings |
| title_short | A Fast Scoring of Human Primary Respiratory Epithelia Grown at Air–Liquid Interface (ALI) to Assess Epithelial Morphology in Research and Personalized Medicine Settings |
| title_sort | fast scoring of human primary respiratory epithelia grown at air liquid interface ali to assess epithelial morphology in research and personalized medicine settings |
| topic | primary respiratory epithelia air–liquid interface culture morphology score goblet cells |
| url | https://www.mdpi.com/2075-4426/14/1/109 |
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