Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria
Abstract Background Non-photosynthetic plastids of plants are known to be involved in a range of metabolic and biosynthetic reactions, even if they have been difficult to study due to their small size and lack of color. The morphology of root plastids is heterogeneous and also the plastid size, dens...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-09-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-020-02635-6 |
| _version_ | 1818843071568150528 |
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| author | Magda Grabsztunowicz Anne Rokka Irum Farooq Eva-Mari Aro Paula Mulo |
| author_facet | Magda Grabsztunowicz Anne Rokka Irum Farooq Eva-Mari Aro Paula Mulo |
| author_sort | Magda Grabsztunowicz |
| collection | DOAJ |
| description | Abstract Background Non-photosynthetic plastids of plants are known to be involved in a range of metabolic and biosynthetic reactions, even if they have been difficult to study due to their small size and lack of color. The morphology of root plastids is heterogeneous and also the plastid size, density and subcellular distribution varies depending on the cell type and developmental stage, and therefore the functional features have remained obscure. Although the root plastid proteome is likely to reveal specific functional features, Arabidopsis thaliana root plastid proteome has not been studied to date. Results In the present study, we separated Arabidopsis root protein fraction enriched with plastids and mitochondria by 2D-PAGE and identified 84 plastid-targeted and 77 mitochondrion-targeted proteins using LC-MS/MS. The most prevalent root plastid protein categories represented amino acid biosynthesis, carbohydrate metabolism and lipid biosynthesis pathways, while the enzymes involved in starch and sucrose metabolism were not detected. Mitochondrion-targeted proteins were classified mainly into the energetics category. Conclusions This is the first study presenting gel-based map of Arabidopsis thaliana root plastid and mitochondrial proteome. Our findings suggest that Arabidopsis root plastids have broad biosynthetic capacity, and that they do not play a major role in a long-term storage of carbohydrates. The proteomic map provides a tool for further studies to compare changes in the proteome, e.g. in response to environmental cues, and emphasizes the role of root plastids in nitrogen and sulfur metabolism as well as in amino acid and fatty acid biosynthesis. The results enable taking a first step towards an integrated view of root plastid/mitochondrial proteome and metabolic functions in Arabidopsis thaliana roots. |
| first_indexed | 2024-12-19T04:52:02Z |
| format | Article |
| id | doaj.art-d6c33cb10cd54df086f53695e76b322d |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-19T04:52:02Z |
| publishDate | 2020-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-d6c33cb10cd54df086f53695e76b322d2022-12-21T20:35:19ZengBMCBMC Plant Biology1471-22292020-09-0120111610.1186/s12870-020-02635-6Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondriaMagda Grabsztunowicz0Anne Rokka1Irum Farooq2Eva-Mari Aro3Paula Mulo4Molecular Plant Biology, University of TurkuTurku Bioscience Centre, University of Turku and Åbo Akademi UniversityMolecular Plant Biology, University of TurkuMolecular Plant Biology, University of TurkuMolecular Plant Biology, University of TurkuAbstract Background Non-photosynthetic plastids of plants are known to be involved in a range of metabolic and biosynthetic reactions, even if they have been difficult to study due to their small size and lack of color. The morphology of root plastids is heterogeneous and also the plastid size, density and subcellular distribution varies depending on the cell type and developmental stage, and therefore the functional features have remained obscure. Although the root plastid proteome is likely to reveal specific functional features, Arabidopsis thaliana root plastid proteome has not been studied to date. Results In the present study, we separated Arabidopsis root protein fraction enriched with plastids and mitochondria by 2D-PAGE and identified 84 plastid-targeted and 77 mitochondrion-targeted proteins using LC-MS/MS. The most prevalent root plastid protein categories represented amino acid biosynthesis, carbohydrate metabolism and lipid biosynthesis pathways, while the enzymes involved in starch and sucrose metabolism were not detected. Mitochondrion-targeted proteins were classified mainly into the energetics category. Conclusions This is the first study presenting gel-based map of Arabidopsis thaliana root plastid and mitochondrial proteome. Our findings suggest that Arabidopsis root plastids have broad biosynthetic capacity, and that they do not play a major role in a long-term storage of carbohydrates. The proteomic map provides a tool for further studies to compare changes in the proteome, e.g. in response to environmental cues, and emphasizes the role of root plastids in nitrogen and sulfur metabolism as well as in amino acid and fatty acid biosynthesis. The results enable taking a first step towards an integrated view of root plastid/mitochondrial proteome and metabolic functions in Arabidopsis thaliana roots.http://link.springer.com/article/10.1186/s12870-020-02635-62-D gel electrophoresisArabidopsis thalianaMass spectrometryMitochondriaProteomicsRoot |
| spellingShingle | Magda Grabsztunowicz Anne Rokka Irum Farooq Eva-Mari Aro Paula Mulo Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria BMC Plant Biology 2-D gel electrophoresis Arabidopsis thaliana Mass spectrometry Mitochondria Proteomics Root |
| title | Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria |
| title_full | Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria |
| title_fullStr | Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria |
| title_full_unstemmed | Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria |
| title_short | Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria |
| title_sort | gel based proteomic map of arabidopsis thaliana root plastids and mitochondria |
| topic | 2-D gel electrophoresis Arabidopsis thaliana Mass spectrometry Mitochondria Proteomics Root |
| url | http://link.springer.com/article/10.1186/s12870-020-02635-6 |
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