miR-148a targets XBP1 to regulate trophoblast apoptosis induced by plasma reticulum stress in preeclampsia
Abstract. To study the relationship between miR-148a and preeclampsia (PE), and clarify that miR-148a can regulate the endoplasmic reticulum stress (ERS) of placental trophoblasts by targeting the ERS protein X box binding protein 1 (XBP1). Fifty patients with hypertension during pregnancy, patients...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer
2022-04-01
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| Series: | Medicine |
| Online Access: | http://journals.lww.com/10.1097/MD.0000000000029130 |
| _version_ | 1818264344336531456 |
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| author | Na Li, MM Jinqiong Li, MM Jiantao Jia, PhD Xia Yao, MM Yan Zhu, MM Yan Li |
| author_facet | Na Li, MM Jinqiong Li, MM Jiantao Jia, PhD Xia Yao, MM Yan Zhu, MM Yan Li |
| author_sort | Na Li, MM |
| collection | DOAJ |
| description | Abstract. To study the relationship between miR-148a and preeclampsia (PE), and clarify that miR-148a can regulate the endoplasmic reticulum stress (ERS) of placental trophoblasts by targeting the ERS protein X box binding protein 1 (XBP1).
Fifty patients with hypertension during pregnancy, patients with mild PE, patients with severe PE, and normal pregnant women were selected, and their placental tissues were collected. RT-polymerase chain reaction was used to detect the expression of miR-148a in placental tissues, and Western blot was used to detect XBP1 in placental tissues. Compare the expression differences of miR-148a and XBP1 in each group, and analyze the correlation between the expressions of the two.
Compared with the Neg-miR group, MTT experiment result in pre-miR-148a group was decreased. MTT experiment result in anti-miR-148a group was increased. Cell cycle test result in pre-miR-148a group [G1 (%)] was increased. Cell cycle test result in anti-miR-148a group [S (%)] was increased. Apoptosis test result in pre-miR-148a group [early apoptotic cells (%), late apoptotic cells (%)] was increased. Apoptosis test result in anti-miR-148a group [early apoptotic cells (%), late apoptotic cells (%)] was decreased. XBP1 expression result in pre-miR-148a group was increased. XBP1 expression result in anti-miR-148a group was decreased. Compared with the normal population, XBP1 is expressed in hypertension, mild eclampsia, severe eclampsia increased. GRP78, CHOP, and caspase-12 expression result in pre-miR-148a group was increased. GRP78, CHOP, and caspase-12 expression result in anti-miR-148a group was decreased.
miR-148a can regulate the ERS-mediated apoptosis by targeting XBP1, thereby intervening in the occurrence and development of PE. |
| first_indexed | 2024-12-12T19:33:25Z |
| format | Article |
| id | doaj.art-d6c388941b0a4c86a58a193f30130b06 |
| institution | Directory Open Access Journal |
| issn | 0025-7974 1536-5964 |
| language | English |
| last_indexed | 2024-12-12T19:33:25Z |
| publishDate | 2022-04-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Medicine |
| spelling | doaj.art-d6c388941b0a4c86a58a193f30130b062022-12-22T00:14:22ZengWolters KluwerMedicine0025-79741536-59642022-04-0110113e2913010.1097/MD.0000000000029130202204010-00007miR-148a targets XBP1 to regulate trophoblast apoptosis induced by plasma reticulum stress in preeclampsiaNa Li, MMJinqiong Li, MMJiantao Jia, PhDXia Yao, MMYan Zhu, MMYan LiAbstract. To study the relationship between miR-148a and preeclampsia (PE), and clarify that miR-148a can regulate the endoplasmic reticulum stress (ERS) of placental trophoblasts by targeting the ERS protein X box binding protein 1 (XBP1). Fifty patients with hypertension during pregnancy, patients with mild PE, patients with severe PE, and normal pregnant women were selected, and their placental tissues were collected. RT-polymerase chain reaction was used to detect the expression of miR-148a in placental tissues, and Western blot was used to detect XBP1 in placental tissues. Compare the expression differences of miR-148a and XBP1 in each group, and analyze the correlation between the expressions of the two. Compared with the Neg-miR group, MTT experiment result in pre-miR-148a group was decreased. MTT experiment result in anti-miR-148a group was increased. Cell cycle test result in pre-miR-148a group [G1 (%)] was increased. Cell cycle test result in anti-miR-148a group [S (%)] was increased. Apoptosis test result in pre-miR-148a group [early apoptotic cells (%), late apoptotic cells (%)] was increased. Apoptosis test result in anti-miR-148a group [early apoptotic cells (%), late apoptotic cells (%)] was decreased. XBP1 expression result in pre-miR-148a group was increased. XBP1 expression result in anti-miR-148a group was decreased. Compared with the normal population, XBP1 is expressed in hypertension, mild eclampsia, severe eclampsia increased. GRP78, CHOP, and caspase-12 expression result in pre-miR-148a group was increased. GRP78, CHOP, and caspase-12 expression result in anti-miR-148a group was decreased. miR-148a can regulate the ERS-mediated apoptosis by targeting XBP1, thereby intervening in the occurrence and development of PE.http://journals.lww.com/10.1097/MD.0000000000029130 |
| spellingShingle | Na Li, MM Jinqiong Li, MM Jiantao Jia, PhD Xia Yao, MM Yan Zhu, MM Yan Li miR-148a targets XBP1 to regulate trophoblast apoptosis induced by plasma reticulum stress in preeclampsia Medicine |
| title | miR-148a targets XBP1 to regulate trophoblast apoptosis induced by plasma reticulum stress in preeclampsia |
| title_full | miR-148a targets XBP1 to regulate trophoblast apoptosis induced by plasma reticulum stress in preeclampsia |
| title_fullStr | miR-148a targets XBP1 to regulate trophoblast apoptosis induced by plasma reticulum stress in preeclampsia |
| title_full_unstemmed | miR-148a targets XBP1 to regulate trophoblast apoptosis induced by plasma reticulum stress in preeclampsia |
| title_short | miR-148a targets XBP1 to regulate trophoblast apoptosis induced by plasma reticulum stress in preeclampsia |
| title_sort | mir 148a targets xbp1 to regulate trophoblast apoptosis induced by plasma reticulum stress in preeclampsia |
| url | http://journals.lww.com/10.1097/MD.0000000000029130 |
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