Development of PCR-Based Markers to Determine the Sex of Kelps.
Sex discriminating genetic markers are commonly used to facilitate breeding programs in economically and ecologically important animal and plant species. However, despite their considerable economic and ecological value, the development of sex markers for kelp species has been very limited. In this...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4619726?pdf=render |
| _version_ | 1819232218803863552 |
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| author | Agnieszka P Lipinska Sophia Ahmed Akira F Peters Sylvain Faugeron J Mark Cock Susana M Coelho |
| author_facet | Agnieszka P Lipinska Sophia Ahmed Akira F Peters Sylvain Faugeron J Mark Cock Susana M Coelho |
| author_sort | Agnieszka P Lipinska |
| collection | DOAJ |
| description | Sex discriminating genetic markers are commonly used to facilitate breeding programs in economically and ecologically important animal and plant species. However, despite their considerable economic and ecological value, the development of sex markers for kelp species has been very limited. In this study, we used the recently described sequence of the sex determining region (SDR) of the brown algal model Ectocarpus to develop novel DNA-based sex-markers for three commercially relevant kelps: Laminaria digitata, Undaria pinnatifida and Macrocystis pyrifera. Markers were designed within nine protein coding genes of Ectocarpus male and female (U/V) sex chromosomes and tested on gametophytes of the three kelp species. Seven primer pairs corresponding to three loci in the Ectocarpus SDR amplified sex-specific bands in the three kelp species, yielding at least one male and one female marker for each species. Our work has generated the first male sex-specific markers for L. digitata and U. pinnatifida, as well as the first sex markers developed for the genus Macrocystis. The markers and methodology presented here will not only facilitate seaweed breeding programs but also represent useful tools for population and demography studies and provide a means to investigate the evolution of sex determination across this largely understudied eukaryotic group. |
| first_indexed | 2024-12-23T11:57:22Z |
| format | Article |
| id | doaj.art-d6c4484512c3430089fa90b9a205bf03 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T11:57:22Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d6c4484512c3430089fa90b9a205bf032022-12-21T17:48:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011010e014053510.1371/journal.pone.0140535Development of PCR-Based Markers to Determine the Sex of Kelps.Agnieszka P LipinskaSophia AhmedAkira F PetersSylvain FaugeronJ Mark CockSusana M CoelhoSex discriminating genetic markers are commonly used to facilitate breeding programs in economically and ecologically important animal and plant species. However, despite their considerable economic and ecological value, the development of sex markers for kelp species has been very limited. In this study, we used the recently described sequence of the sex determining region (SDR) of the brown algal model Ectocarpus to develop novel DNA-based sex-markers for three commercially relevant kelps: Laminaria digitata, Undaria pinnatifida and Macrocystis pyrifera. Markers were designed within nine protein coding genes of Ectocarpus male and female (U/V) sex chromosomes and tested on gametophytes of the three kelp species. Seven primer pairs corresponding to three loci in the Ectocarpus SDR amplified sex-specific bands in the three kelp species, yielding at least one male and one female marker for each species. Our work has generated the first male sex-specific markers for L. digitata and U. pinnatifida, as well as the first sex markers developed for the genus Macrocystis. The markers and methodology presented here will not only facilitate seaweed breeding programs but also represent useful tools for population and demography studies and provide a means to investigate the evolution of sex determination across this largely understudied eukaryotic group.http://europepmc.org/articles/PMC4619726?pdf=render |
| spellingShingle | Agnieszka P Lipinska Sophia Ahmed Akira F Peters Sylvain Faugeron J Mark Cock Susana M Coelho Development of PCR-Based Markers to Determine the Sex of Kelps. PLoS ONE |
| title | Development of PCR-Based Markers to Determine the Sex of Kelps. |
| title_full | Development of PCR-Based Markers to Determine the Sex of Kelps. |
| title_fullStr | Development of PCR-Based Markers to Determine the Sex of Kelps. |
| title_full_unstemmed | Development of PCR-Based Markers to Determine the Sex of Kelps. |
| title_short | Development of PCR-Based Markers to Determine the Sex of Kelps. |
| title_sort | development of pcr based markers to determine the sex of kelps |
| url | http://europepmc.org/articles/PMC4619726?pdf=render |
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