Long-pulsed ultrasound-mediated microbubble thrombolysis in a rat model of microvascular obstruction
In up to 30% patients who experience acute myocardial infarction, successful recanalization of the epicardial coronary artery cannot provide adequate microvascular reperfusion. In this study, we sought to determine whether long-pulsed ultrasound (US)-mediated microbubble (MB) cavitation was useful f...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2024-03-01
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| Series: | Open Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/med-2024-0935 |
| _version_ | 1797229683704594432 |
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| author | Wang Rui Chen Xianghui Zha Daogang |
| author_facet | Wang Rui Chen Xianghui Zha Daogang |
| author_sort | Wang Rui |
| collection | DOAJ |
| description | In up to 30% patients who experience acute myocardial infarction, successful recanalization of the epicardial coronary artery cannot provide adequate microvascular reperfusion. In this study, we sought to determine whether long-pulsed ultrasound (US)-mediated microbubble (MB) cavitation was useful for the treatment of microvascular obstruction, and the therapeutic effects were compared within different long-pulse-length and short-pulsed US. Microvascular obstruction model was established by injecting micro-thrombi into common iliac artery of a rat’s hind limb. About 1 MHz US with different long pulse lengths (ranging from 100 to 50,000 cycles) was delivered, compared to short pulse (5 cycles). The control group was given MB only without therapeutic US. Contrast perfusion images were performed at baseline, emboli, and 1, 5, 10 min post-embolization, and peak plateau video intensity (A) was obtained to evaluate the therapeutic effects. Long-tone-burst US showed better thrombolytic effects than short-pulsed US (1,000, 5,000 cycles >500 cycles, >5 cycles, and control) (P < 0.01). 1,000 cycles group showed the optimal thrombolytic effect, but microvascular hemorrhage was observed in 50,000 cycles group. In conclusion, long-tone-burst US-enhanced MB therapy mediated successful thrombolysis and may offer a powerful approach for the treatment for microvascular obstruction within a certain pulse length. |
| first_indexed | 2024-04-24T15:16:30Z |
| format | Article |
| id | doaj.art-d6c5641042c649819b06c08c8360d179 |
| institution | Directory Open Access Journal |
| issn | 2391-5463 |
| language | English |
| last_indexed | 2024-04-24T15:16:30Z |
| publishDate | 2024-03-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Medicine |
| spelling | doaj.art-d6c5641042c649819b06c08c8360d1792024-04-02T09:20:26ZengDe GruyterOpen Medicine2391-54632024-03-011912819210.1515/med-2024-0935Long-pulsed ultrasound-mediated microbubble thrombolysis in a rat model of microvascular obstructionWang Rui0Chen Xianghui1Zha Daogang2Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Cardiology, The First Affiliated Hospital of Jinan University, No. 613 Huangpu West Avenue, Guangzhou, Guangdong, ChinaDepartment of General Practice, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaIn up to 30% patients who experience acute myocardial infarction, successful recanalization of the epicardial coronary artery cannot provide adequate microvascular reperfusion. In this study, we sought to determine whether long-pulsed ultrasound (US)-mediated microbubble (MB) cavitation was useful for the treatment of microvascular obstruction, and the therapeutic effects were compared within different long-pulse-length and short-pulsed US. Microvascular obstruction model was established by injecting micro-thrombi into common iliac artery of a rat’s hind limb. About 1 MHz US with different long pulse lengths (ranging from 100 to 50,000 cycles) was delivered, compared to short pulse (5 cycles). The control group was given MB only without therapeutic US. Contrast perfusion images were performed at baseline, emboli, and 1, 5, 10 min post-embolization, and peak plateau video intensity (A) was obtained to evaluate the therapeutic effects. Long-tone-burst US showed better thrombolytic effects than short-pulsed US (1,000, 5,000 cycles >500 cycles, >5 cycles, and control) (P < 0.01). 1,000 cycles group showed the optimal thrombolytic effect, but microvascular hemorrhage was observed in 50,000 cycles group. In conclusion, long-tone-burst US-enhanced MB therapy mediated successful thrombolysis and may offer a powerful approach for the treatment for microvascular obstruction within a certain pulse length.https://doi.org/10.1515/med-2024-0935thrombolysismicrobubblemicrovascular obstructionlong-pulsed ultrasound |
| spellingShingle | Wang Rui Chen Xianghui Zha Daogang Long-pulsed ultrasound-mediated microbubble thrombolysis in a rat model of microvascular obstruction Open Medicine thrombolysis microbubble microvascular obstruction long-pulsed ultrasound |
| title | Long-pulsed ultrasound-mediated microbubble thrombolysis in a rat model of microvascular obstruction |
| title_full | Long-pulsed ultrasound-mediated microbubble thrombolysis in a rat model of microvascular obstruction |
| title_fullStr | Long-pulsed ultrasound-mediated microbubble thrombolysis in a rat model of microvascular obstruction |
| title_full_unstemmed | Long-pulsed ultrasound-mediated microbubble thrombolysis in a rat model of microvascular obstruction |
| title_short | Long-pulsed ultrasound-mediated microbubble thrombolysis in a rat model of microvascular obstruction |
| title_sort | long pulsed ultrasound mediated microbubble thrombolysis in a rat model of microvascular obstruction |
| topic | thrombolysis microbubble microvascular obstruction long-pulsed ultrasound |
| url | https://doi.org/10.1515/med-2024-0935 |
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