Preparation and in vitro characterization of electrospun scaffolds composed of chitosan, gelatin and 58S bioactive glass nanoparticles for skin tissue engineering
Background and aims: The presence of an appropriate scaffold at the wound site could significantly improve the healing process. In this study, we aimed to prepare a biomimetic nanocomposite scaffold composed of chitosan, gelatin, and 58S bioglass nanoparticles for skin tissue engineering. Methods: T...
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| Format: | Article |
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Shahrekord University of Medical Sciences
2022-03-01
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| Series: | Journal of Shahrekord University of Medical Sciences |
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| Online Access: | http://j.skums.ac.ir/PDF/jskums-24-1.pdf |
| _version_ | 1798024972618170368 |
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| author | Hamed Nosrati Mehdi Banitalebi-Dhkordi Mohammad Khodaei Esmaeel Sharifi Shiva Asadpour Kamran Mansouri Mostafa Soleimannejad |
| author_facet | Hamed Nosrati Mehdi Banitalebi-Dhkordi Mohammad Khodaei Esmaeel Sharifi Shiva Asadpour Kamran Mansouri Mostafa Soleimannejad |
| author_sort | Hamed Nosrati |
| collection | DOAJ |
| description | Background and aims: The presence of an appropriate scaffold at the wound site could significantly improve the healing process. In this study, we aimed to prepare a biomimetic nanocomposite scaffold composed of chitosan, gelatin, and 58S bioglass nanoparticles for skin tissue engineering. Methods: The nanocomposite scaffolds composed of chitosan, gelatin, and 58S bioglass nanoparticles were fabricated through electrospinning process. Then the cell viability assay was performed in order to evaluate the biological properties of the membranes. The optimum concentration of bioglass nanoparticles was determined for further studies. In vitro characterization was also performed to evaluate physicochemical properties of the scaffolds. Results: The chitosan/gelatin scaffold containing 2% of 58S bioglass nanoparticles showed no cell toxicity, and the dermal fibroblasts were found capable of proliferation on the membrane. The in vitro results obtained from the scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and porosity tests demonstrated the appropriate properties of the membrane as a scaffold for skin regeneration. Conclusions: It was concluded that a chitosan-gelatin membrane containing 2% of 58S bioglass nanoparticles had the potential to function as a scaffold to accelerate wound healing due to its suitable properties, such as high porosity, high surface/volume ratio, and excellent biocompatibility. |
| first_indexed | 2024-04-11T18:11:16Z |
| format | Article |
| id | doaj.art-d6c708ae9b034c47833db57e6f633b0f |
| institution | Directory Open Access Journal |
| issn | 2717-0071 |
| language | English |
| last_indexed | 2024-04-11T18:11:16Z |
| publishDate | 2022-03-01 |
| publisher | Shahrekord University of Medical Sciences |
| record_format | Article |
| series | Journal of Shahrekord University of Medical Sciences |
| spelling | doaj.art-d6c708ae9b034c47833db57e6f633b0f2022-12-22T04:10:06ZengShahrekord University of Medical SciencesJournal of Shahrekord University of Medical Sciences2717-00712022-03-012411610.34172/jsums.2022.01jskums-419Preparation and in vitro characterization of electrospun scaffolds composed of chitosan, gelatin and 58S bioactive glass nanoparticles for skin tissue engineeringHamed Nosrati0Mehdi Banitalebi-Dhkordi1Mohammad Khodaei2Esmaeel Sharifi3Shiva Asadpour4Kamran Mansouri5Mostafa Soleimannejad6Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, IranCellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, IranMaterials Engineering Group, Golpayegan College of Engineering, Isfahan University of Technology, Golpayegan 87717- 67498, IranDepartment of Tissue Engineering and Biomaterials, School of Advanced Medical Sciences and Technologies, Hamadan University of Medical Sciences, Hamadan, IranDepartment of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, IranMedical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, IranBackground and aims: The presence of an appropriate scaffold at the wound site could significantly improve the healing process. In this study, we aimed to prepare a biomimetic nanocomposite scaffold composed of chitosan, gelatin, and 58S bioglass nanoparticles for skin tissue engineering. Methods: The nanocomposite scaffolds composed of chitosan, gelatin, and 58S bioglass nanoparticles were fabricated through electrospinning process. Then the cell viability assay was performed in order to evaluate the biological properties of the membranes. The optimum concentration of bioglass nanoparticles was determined for further studies. In vitro characterization was also performed to evaluate physicochemical properties of the scaffolds. Results: The chitosan/gelatin scaffold containing 2% of 58S bioglass nanoparticles showed no cell toxicity, and the dermal fibroblasts were found capable of proliferation on the membrane. The in vitro results obtained from the scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and porosity tests demonstrated the appropriate properties of the membrane as a scaffold for skin regeneration. Conclusions: It was concluded that a chitosan-gelatin membrane containing 2% of 58S bioglass nanoparticles had the potential to function as a scaffold to accelerate wound healing due to its suitable properties, such as high porosity, high surface/volume ratio, and excellent biocompatibility.http://j.skums.ac.ir/PDF/jskums-24-1.pdfchitosangelatinbioactive glasswound healingtissue engineering |
| spellingShingle | Hamed Nosrati Mehdi Banitalebi-Dhkordi Mohammad Khodaei Esmaeel Sharifi Shiva Asadpour Kamran Mansouri Mostafa Soleimannejad Preparation and in vitro characterization of electrospun scaffolds composed of chitosan, gelatin and 58S bioactive glass nanoparticles for skin tissue engineering Journal of Shahrekord University of Medical Sciences chitosan gelatin bioactive glass wound healing tissue engineering |
| title | Preparation and in vitro characterization of electrospun scaffolds composed of chitosan, gelatin and 58S bioactive glass nanoparticles for skin tissue engineering |
| title_full | Preparation and in vitro characterization of electrospun scaffolds composed of chitosan, gelatin and 58S bioactive glass nanoparticles for skin tissue engineering |
| title_fullStr | Preparation and in vitro characterization of electrospun scaffolds composed of chitosan, gelatin and 58S bioactive glass nanoparticles for skin tissue engineering |
| title_full_unstemmed | Preparation and in vitro characterization of electrospun scaffolds composed of chitosan, gelatin and 58S bioactive glass nanoparticles for skin tissue engineering |
| title_short | Preparation and in vitro characterization of electrospun scaffolds composed of chitosan, gelatin and 58S bioactive glass nanoparticles for skin tissue engineering |
| title_sort | preparation and in vitro characterization of electrospun scaffolds composed of chitosan gelatin and 58s bioactive glass nanoparticles for skin tissue engineering |
| topic | chitosan gelatin bioactive glass wound healing tissue engineering |
| url | http://j.skums.ac.ir/PDF/jskums-24-1.pdf |
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