Slicing Spheroids in Microfluidic Devices for Morphological and Immunohistochemical Analysis
Microfluidic devices utilizing spheroids play important roles in in vitro experimental systems to closely simulate morphological and biochemical characteristics of the in vivo tumor microenvironment. For the observation and analysis of the inner structure of spheroids, sectioning is an efficient app...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/11/5/480 |
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| author | Satoru Kuriu Tetsuya Kadonosono Shinae Kizaka-Kondoh Tadashi Ishida |
| author_facet | Satoru Kuriu Tetsuya Kadonosono Shinae Kizaka-Kondoh Tadashi Ishida |
| author_sort | Satoru Kuriu |
| collection | DOAJ |
| description | Microfluidic devices utilizing spheroids play important roles in in vitro experimental systems to closely simulate morphological and biochemical characteristics of the in vivo tumor microenvironment. For the observation and analysis of the inner structure of spheroids, sectioning is an efficient approach. However, conventional microfluidic devices are difficult for sectioning, and therefore, spheroids inside the microfluidic channels have not been sliced well. We proposed a microfluidic device created from embedding resin for sectioning. Spheroids were cultured, embedded by resin, and sectioned in the microfluidic device. Slices of the sectioned spheroids yielded clear images at the cellular level. According to morphological and immunohistochemical analyses of the slices of the spheroid, specific protein distribution was observed. |
| first_indexed | 2024-03-10T20:00:44Z |
| format | Article |
| id | doaj.art-d6c7886700b54d12be68af1329f30102 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T20:00:44Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-d6c7886700b54d12be68af1329f301022023-11-19T23:37:35ZengMDPI AGMicromachines2072-666X2020-05-0111548010.3390/mi11050480Slicing Spheroids in Microfluidic Devices for Morphological and Immunohistochemical AnalysisSatoru Kuriu0Tetsuya Kadonosono1Shinae Kizaka-Kondoh2Tadashi Ishida3Department of Mechanical Engineering, School of Engineering, Tokyo Institute of Technology, Kanagawa 226-8503, JapanDepartment of Life Science and Technology, School of Life Science and Technology, Tokyo Institute of Technology, Kanagawa 226-8503, JapanDepartment of Life Science and Technology, School of Life Science and Technology, Tokyo Institute of Technology, Kanagawa 226-8503, JapanDepartment of Mechanical Engineering, School of Engineering, Tokyo Institute of Technology, Kanagawa 226-8503, JapanMicrofluidic devices utilizing spheroids play important roles in in vitro experimental systems to closely simulate morphological and biochemical characteristics of the in vivo tumor microenvironment. For the observation and analysis of the inner structure of spheroids, sectioning is an efficient approach. However, conventional microfluidic devices are difficult for sectioning, and therefore, spheroids inside the microfluidic channels have not been sliced well. We proposed a microfluidic device created from embedding resin for sectioning. Spheroids were cultured, embedded by resin, and sectioned in the microfluidic device. Slices of the sectioned spheroids yielded clear images at the cellular level. According to morphological and immunohistochemical analyses of the slices of the spheroid, specific protein distribution was observed.https://www.mdpi.com/2072-666X/11/5/480microfluidic deviceembedding resinspheroidsectioningimmunohistochemistry |
| spellingShingle | Satoru Kuriu Tetsuya Kadonosono Shinae Kizaka-Kondoh Tadashi Ishida Slicing Spheroids in Microfluidic Devices for Morphological and Immunohistochemical Analysis Micromachines microfluidic device embedding resin spheroid sectioning immunohistochemistry |
| title | Slicing Spheroids in Microfluidic Devices for Morphological and Immunohistochemical Analysis |
| title_full | Slicing Spheroids in Microfluidic Devices for Morphological and Immunohistochemical Analysis |
| title_fullStr | Slicing Spheroids in Microfluidic Devices for Morphological and Immunohistochemical Analysis |
| title_full_unstemmed | Slicing Spheroids in Microfluidic Devices for Morphological and Immunohistochemical Analysis |
| title_short | Slicing Spheroids in Microfluidic Devices for Morphological and Immunohistochemical Analysis |
| title_sort | slicing spheroids in microfluidic devices for morphological and immunohistochemical analysis |
| topic | microfluidic device embedding resin spheroid sectioning immunohistochemistry |
| url | https://www.mdpi.com/2072-666X/11/5/480 |
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