Iron supplementation inhibits hypoxia-induced mitochondrial damage and protects zebrafish liver cells from death
Acute hypoxia in water has always been a thorny problem in aquaculture. Oxygen and iron play important roles and are interdependent in fish. Iron is essential for oxygen transport and its concentration tightly controlled to maintain the cellular redox homeostasis. However, it is still unclear the ro...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.925752/full |
| _version_ | 1798036606923309056 |
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| author | Ruiqin Hu Ruiqin Hu Genfang Li Genfang Li Qianghua Xu Qianghua Xu Qianghua Xu Liangbiao Chen Liangbiao Chen |
| author_facet | Ruiqin Hu Ruiqin Hu Genfang Li Genfang Li Qianghua Xu Qianghua Xu Qianghua Xu Liangbiao Chen Liangbiao Chen |
| author_sort | Ruiqin Hu |
| collection | DOAJ |
| description | Acute hypoxia in water has always been a thorny problem in aquaculture. Oxygen and iron play important roles and are interdependent in fish. Iron is essential for oxygen transport and its concentration tightly controlled to maintain the cellular redox homeostasis. However, it is still unclear the role and mechanism of iron in hypoxic stress of fish. In this study, we investigated the role of iron in hypoxic responses of two zebrafish-derived cell lines. We found hypoxia exposed zebrafish liver cells (ZFL) demonstrated reduced expression of Ferritin and the gene fth31 for mitochondrial iron storage, corresponding to reduction of both intracellular and mitochondrial free iron and significant decrease of ROS levels in multiple cellular components, including mitochondrial ROS and lipid peroxidation level. In parallel, the mitochondrial integrity was severely damaged. Addition of exogenous iron restored the iron and ROS levels in cellular and mitochondria, reduced mitochondrial damage through enhancing mitophagy leading to higher cell viability, while treated the cells with iron chelator (DFO) or ferroptosis inhibitor (Fer-1) showed no improvements of the cellular conditions. In contrast, in hypoxia insensitive zebrafish embryonic fibroblasts cells (ZF4), the expression of genes related to iron metabolism showed opposite trends of change and higher mitochondrial ROS level compared with the ZFL cells. These results suggest that iron homeostasis is important for zebrafish cells to maintain mitochondrial integrity in hypoxic stress, which is cell type dependent. Our study enriched the hypoxia regulation mechanism of fish, which helped to reduce the hypoxia loss in fish farming. |
| first_indexed | 2024-04-11T21:15:17Z |
| format | Article |
| id | doaj.art-d6c954cc2c424d6482050b8f9b2abddc |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-11T21:15:17Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-d6c954cc2c424d6482050b8f9b2abddc2022-12-22T04:02:51ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-08-011310.3389/fphys.2022.925752925752Iron supplementation inhibits hypoxia-induced mitochondrial damage and protects zebrafish liver cells from deathRuiqin Hu0Ruiqin Hu1Genfang Li2Genfang Li3Qianghua Xu4Qianghua Xu5Qianghua Xu6Liangbiao Chen7Liangbiao Chen8International Joint Research Centre for Marine Biosciences (Ministry of Science and Technology), College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Ministry of Education) and International Research Centre for Marine Biosciences, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaInternational Joint Research Centre for Marine Biosciences (Ministry of Science and Technology), College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Ministry of Education) and International Research Centre for Marine Biosciences, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaInternational Joint Research Centre for Marine Biosciences (Ministry of Science and Technology), College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Ministry of Education) and International Research Centre for Marine Biosciences, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources, College of Marine Science, Shanghai Ocean University, Shanghai, ChinaInternational Joint Research Centre for Marine Biosciences (Ministry of Science and Technology), College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Ministry of Education) and International Research Centre for Marine Biosciences, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaAcute hypoxia in water has always been a thorny problem in aquaculture. Oxygen and iron play important roles and are interdependent in fish. Iron is essential for oxygen transport and its concentration tightly controlled to maintain the cellular redox homeostasis. However, it is still unclear the role and mechanism of iron in hypoxic stress of fish. In this study, we investigated the role of iron in hypoxic responses of two zebrafish-derived cell lines. We found hypoxia exposed zebrafish liver cells (ZFL) demonstrated reduced expression of Ferritin and the gene fth31 for mitochondrial iron storage, corresponding to reduction of both intracellular and mitochondrial free iron and significant decrease of ROS levels in multiple cellular components, including mitochondrial ROS and lipid peroxidation level. In parallel, the mitochondrial integrity was severely damaged. Addition of exogenous iron restored the iron and ROS levels in cellular and mitochondria, reduced mitochondrial damage through enhancing mitophagy leading to higher cell viability, while treated the cells with iron chelator (DFO) or ferroptosis inhibitor (Fer-1) showed no improvements of the cellular conditions. In contrast, in hypoxia insensitive zebrafish embryonic fibroblasts cells (ZF4), the expression of genes related to iron metabolism showed opposite trends of change and higher mitochondrial ROS level compared with the ZFL cells. These results suggest that iron homeostasis is important for zebrafish cells to maintain mitochondrial integrity in hypoxic stress, which is cell type dependent. Our study enriched the hypoxia regulation mechanism of fish, which helped to reduce the hypoxia loss in fish farming.https://www.frontiersin.org/articles/10.3389/fphys.2022.925752/fullhypoxiaironROSmitochondriamitophagy |
| spellingShingle | Ruiqin Hu Ruiqin Hu Genfang Li Genfang Li Qianghua Xu Qianghua Xu Qianghua Xu Liangbiao Chen Liangbiao Chen Iron supplementation inhibits hypoxia-induced mitochondrial damage and protects zebrafish liver cells from death Frontiers in Physiology hypoxia iron ROS mitochondria mitophagy |
| title | Iron supplementation inhibits hypoxia-induced mitochondrial damage and protects zebrafish liver cells from death |
| title_full | Iron supplementation inhibits hypoxia-induced mitochondrial damage and protects zebrafish liver cells from death |
| title_fullStr | Iron supplementation inhibits hypoxia-induced mitochondrial damage and protects zebrafish liver cells from death |
| title_full_unstemmed | Iron supplementation inhibits hypoxia-induced mitochondrial damage and protects zebrafish liver cells from death |
| title_short | Iron supplementation inhibits hypoxia-induced mitochondrial damage and protects zebrafish liver cells from death |
| title_sort | iron supplementation inhibits hypoxia induced mitochondrial damage and protects zebrafish liver cells from death |
| topic | hypoxia iron ROS mitochondria mitophagy |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.925752/full |
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