An FDA-Drug Library Screen for Compounds with Bioactivities against Meticillin-Resistant Staphylococcus aureus (MRSA)
The lack of new antibacterial drugs entering the market and their misuse have resulted in the emergence of drug-resistant bacteria, posing a major health crisis worldwide. In particular, meticillin-resistant Staphylococcus aureus (MRSA), a pathogen responsible for numerous human infections, has beco...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2015-10-01
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| Series: | Antibiotics |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2079-6382/4/4/424 |
| _version_ | 1828529488552525824 |
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| author | Qiu Ying Lau Yoke Yan Fion Tan Vanessa Chai Yin Goh David Jing Qin Lee Fui Mee Ng Esther H. Q. Ong Jeffrey Hill Cheng San Brian Chia |
| author_facet | Qiu Ying Lau Yoke Yan Fion Tan Vanessa Chai Yin Goh David Jing Qin Lee Fui Mee Ng Esther H. Q. Ong Jeffrey Hill Cheng San Brian Chia |
| author_sort | Qiu Ying Lau |
| collection | DOAJ |
| description | The lack of new antibacterial drugs entering the market and their misuse have resulted in the emergence of drug-resistant bacteria, posing a major health crisis worldwide. In particular, meticillin-resistant Staphylococcus aureus (MRSA), a pathogen responsible for numerous human infections, has become endemic in hospitals worldwide. Drug repurposing, the finding of new therapeutic indications for approved drugs, is deemed a plausible solution to accelerate drug discovery and development in this area. Towards this end, we screened 1163 drugs approved by the Food and Drug Administration (FDA) for bioactivities against MRSA in a 10 μM single-point assay. After excluding known antibiotics and antiseptics, six compounds were identified and their MICs were determined against a panel of clinical MRSA strains. A toxicity assay using human keratinocytes was also conducted to gauge their potential for repurposing as topical agents for treating MRSA skin infections. |
| first_indexed | 2024-12-11T22:09:14Z |
| format | Article |
| id | doaj.art-d6c959dd7b6f48a58d34699a85b6f57f |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-12-11T22:09:14Z |
| publishDate | 2015-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-d6c959dd7b6f48a58d34699a85b6f57f2022-12-22T00:48:51ZengMDPI AGAntibiotics2079-63822015-10-014442443410.3390/antibiotics4040424antibiotics4040424An FDA-Drug Library Screen for Compounds with Bioactivities against Meticillin-Resistant Staphylococcus aureus (MRSA)Qiu Ying Lau0Yoke Yan Fion Tan1Vanessa Chai Yin Goh2David Jing Qin Lee3Fui Mee Ng4Esther H. Q. Ong5Jeffrey Hill6Cheng San Brian Chia7Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos #03-01, Singapore 138669, SingaporeExperimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos #03-01, Singapore 138669, SingaporeExperimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos #03-01, Singapore 138669, SingaporeExperimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos #03-01, Singapore 138669, SingaporeExperimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos #03-01, Singapore 138669, SingaporeExperimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos #03-01, Singapore 138669, SingaporeExperimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos #03-01, Singapore 138669, SingaporeExperimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos #03-01, Singapore 138669, SingaporeThe lack of new antibacterial drugs entering the market and their misuse have resulted in the emergence of drug-resistant bacteria, posing a major health crisis worldwide. In particular, meticillin-resistant Staphylococcus aureus (MRSA), a pathogen responsible for numerous human infections, has become endemic in hospitals worldwide. Drug repurposing, the finding of new therapeutic indications for approved drugs, is deemed a plausible solution to accelerate drug discovery and development in this area. Towards this end, we screened 1163 drugs approved by the Food and Drug Administration (FDA) for bioactivities against MRSA in a 10 μM single-point assay. After excluding known antibiotics and antiseptics, six compounds were identified and their MICs were determined against a panel of clinical MRSA strains. A toxicity assay using human keratinocytes was also conducted to gauge their potential for repurposing as topical agents for treating MRSA skin infections.http://www.mdpi.com/2079-6382/4/4/424drug library screendrug repurposingdrug repositioningMRSA |
| spellingShingle | Qiu Ying Lau Yoke Yan Fion Tan Vanessa Chai Yin Goh David Jing Qin Lee Fui Mee Ng Esther H. Q. Ong Jeffrey Hill Cheng San Brian Chia An FDA-Drug Library Screen for Compounds with Bioactivities against Meticillin-Resistant Staphylococcus aureus (MRSA) Antibiotics drug library screen drug repurposing drug repositioning MRSA |
| title | An FDA-Drug Library Screen for Compounds with Bioactivities against Meticillin-Resistant Staphylococcus aureus (MRSA) |
| title_full | An FDA-Drug Library Screen for Compounds with Bioactivities against Meticillin-Resistant Staphylococcus aureus (MRSA) |
| title_fullStr | An FDA-Drug Library Screen for Compounds with Bioactivities against Meticillin-Resistant Staphylococcus aureus (MRSA) |
| title_full_unstemmed | An FDA-Drug Library Screen for Compounds with Bioactivities against Meticillin-Resistant Staphylococcus aureus (MRSA) |
| title_short | An FDA-Drug Library Screen for Compounds with Bioactivities against Meticillin-Resistant Staphylococcus aureus (MRSA) |
| title_sort | fda drug library screen for compounds with bioactivities against meticillin resistant staphylococcus aureus mrsa |
| topic | drug library screen drug repurposing drug repositioning MRSA |
| url | http://www.mdpi.com/2079-6382/4/4/424 |
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