Application of Au@Pt Nanozyme as Enhancing Label for the Sensitive Lateral Flow Immunoassay of Okadaic Acid
In this study, a lateral flow immunoassay (LFIA) was developed to detect okadaic acid (OA) belonging to the diarrheic shellfish poisoning group of aquatic toxins. Newly obtained anti-OA monoclonal antibodies and bimetallic core@shell Au@Pt nanoparticles were used in the indirect format of the LFIA....
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/12/1137 |
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| author | Olga D. Hendrickson Elena A. Zvereva Vasily G. Panferov Olga N. Solopova Anatoly V. Zherdev Peter G. Sveshnikov Boris B. Dzantiev |
| author_facet | Olga D. Hendrickson Elena A. Zvereva Vasily G. Panferov Olga N. Solopova Anatoly V. Zherdev Peter G. Sveshnikov Boris B. Dzantiev |
| author_sort | Olga D. Hendrickson |
| collection | DOAJ |
| description | In this study, a lateral flow immunoassay (LFIA) was developed to detect okadaic acid (OA) belonging to the diarrheic shellfish poisoning group of aquatic toxins. Newly obtained anti-OA monoclonal antibodies and bimetallic core@shell Au@Pt nanoparticles were used in the indirect format of the LFIA. Peroxidase-mimicking nanozyme properties of Au@Pt enabled using them to enhance band coloration on the test strips and, consequently, for increasing the LFIA sensitivity. The instrumental limit of detection (LOD), the working range of detectable concentrations, and the visual cutoff of the assay were 0.5, 0.8–6.8, and 10 ng/mL, respectively. The assay duration was 20 min. The rapid and simple sample preparation procedure was applied for seawater, river water, and fish samples. The total duration of the sample pretreatment and LFIA was 25/40 min for water/fish samples, ensuring testing rapidity. The developed test system provides sensitive control of raw materials and food products and can be used to detect OA at all stages of the food industry «from sea to fork» chains. |
| first_indexed | 2024-03-09T17:16:39Z |
| format | Article |
| id | doaj.art-d6c96b66c196470ca48d3b58af971217 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T17:16:39Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-d6c96b66c196470ca48d3b58af9712172023-11-24T13:37:10ZengMDPI AGBiosensors2079-63742022-12-011212113710.3390/bios12121137Application of Au@Pt Nanozyme as Enhancing Label for the Sensitive Lateral Flow Immunoassay of Okadaic AcidOlga D. Hendrickson0Elena A. Zvereva1Vasily G. Panferov2Olga N. Solopova3Anatoly V. Zherdev4Peter G. Sveshnikov5Boris B. Dzantiev6Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaBach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaBach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaBlokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Kashirskoye Shosse 24, 115478 Moscow, RussiaBach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaRussian Research Center for Molecular Diagnostics and Therapy, Sympheropolsky Blvrd., 8, 117638 Moscow, RussiaBach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, RussiaIn this study, a lateral flow immunoassay (LFIA) was developed to detect okadaic acid (OA) belonging to the diarrheic shellfish poisoning group of aquatic toxins. Newly obtained anti-OA monoclonal antibodies and bimetallic core@shell Au@Pt nanoparticles were used in the indirect format of the LFIA. Peroxidase-mimicking nanozyme properties of Au@Pt enabled using them to enhance band coloration on the test strips and, consequently, for increasing the LFIA sensitivity. The instrumental limit of detection (LOD), the working range of detectable concentrations, and the visual cutoff of the assay were 0.5, 0.8–6.8, and 10 ng/mL, respectively. The assay duration was 20 min. The rapid and simple sample preparation procedure was applied for seawater, river water, and fish samples. The total duration of the sample pretreatment and LFIA was 25/40 min for water/fish samples, ensuring testing rapidity. The developed test system provides sensitive control of raw materials and food products and can be used to detect OA at all stages of the food industry «from sea to fork» chains.https://www.mdpi.com/2079-6374/12/12/1137aquatic toxinsokadaic acidimmunochromatographynanozymefood safety |
| spellingShingle | Olga D. Hendrickson Elena A. Zvereva Vasily G. Panferov Olga N. Solopova Anatoly V. Zherdev Peter G. Sveshnikov Boris B. Dzantiev Application of Au@Pt Nanozyme as Enhancing Label for the Sensitive Lateral Flow Immunoassay of Okadaic Acid Biosensors aquatic toxins okadaic acid immunochromatography nanozyme food safety |
| title | Application of Au@Pt Nanozyme as Enhancing Label for the Sensitive Lateral Flow Immunoassay of Okadaic Acid |
| title_full | Application of Au@Pt Nanozyme as Enhancing Label for the Sensitive Lateral Flow Immunoassay of Okadaic Acid |
| title_fullStr | Application of Au@Pt Nanozyme as Enhancing Label for the Sensitive Lateral Flow Immunoassay of Okadaic Acid |
| title_full_unstemmed | Application of Au@Pt Nanozyme as Enhancing Label for the Sensitive Lateral Flow Immunoassay of Okadaic Acid |
| title_short | Application of Au@Pt Nanozyme as Enhancing Label for the Sensitive Lateral Flow Immunoassay of Okadaic Acid |
| title_sort | application of au pt nanozyme as enhancing label for the sensitive lateral flow immunoassay of okadaic acid |
| topic | aquatic toxins okadaic acid immunochromatography nanozyme food safety |
| url | https://www.mdpi.com/2079-6374/12/12/1137 |
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