Oral Ingestion of Yuzu Seed Oil Suppresses the Development of Atopic Dermatitis-like Skin Lesions in NC/Nga Mice
Long-term oral ingestion of unheated yuzu seed oil in humans reduces lipid peroxides in the blood. Moreover, yuzu seed oil contains limonin, which can induce antioxidant and anti-inflammatory effects by activating the transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2). Previousl...
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MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/5/2689 |
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| author | Kimito Asano Yoshiya Watanabe Mio Miyamoto Mochifumi Toutani Shunji Mizobuchi |
| author_facet | Kimito Asano Yoshiya Watanabe Mio Miyamoto Mochifumi Toutani Shunji Mizobuchi |
| author_sort | Kimito Asano |
| collection | DOAJ |
| description | Long-term oral ingestion of unheated yuzu seed oil in humans reduces lipid peroxides in the blood. Moreover, yuzu seed oil contains limonin, which can induce antioxidant and anti-inflammatory effects by activating the transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2). Previously, Nrf2 has been shown to reduce atopic dermatitis (AD). Therefore, we hypothesized that ingesting unheated yuzu seed oil can regulate AD through Nrf2. An AD model was established using NC/Nga mice through repeated local exposure to mite antigens. Unheated and purified yuzu seed oil (100 µL/mice) or water (control, 100 µL/mice) was administered orally once a day using a gastric cannula for rodents for 28 days. On day 28, mice in the unheated yuzu seed oil group exhibited significantly lower clinical skin severity scores and ear thickness than those in the purified yuzu seed oil and water groups. Serum histamine levels remained unaltered among the three AD-induced groups. Serum <i>Dermatophagoides farina</i> body (Dfb)-specific immunoglobulin E (IgE) levels were significantly lower in the unheated yuzu seed oil group. Oral ingestion of yuzu seed oil in NC/Nga AD model mice significantly suppressed dermatitis deterioration and decreased serum IgE levels. Clinical trials (<i>n</i> = 41) have already confirmed that unheated yuzu oil is safe for long-term intake, further suggesting its potential use in improving AD symptoms. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-25T00:29:27Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d6ca9eb6e2f740bdbdab1fddc2f432a42024-03-12T16:45:58ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01255268910.3390/ijms25052689Oral Ingestion of Yuzu Seed Oil Suppresses the Development of Atopic Dermatitis-like Skin Lesions in NC/Nga MiceKimito Asano0Yoshiya Watanabe1Mio Miyamoto2Mochifumi Toutani3Shunji Mizobuchi4Kochi-Umajimura Yuzu Health Research Course, Kochi Medical School, Kochi University, Nankoku 783-8505, JapanKochi-Umajimura Yuzu Health Research Course, Kochi Medical School, Kochi University, Nankoku 783-8505, JapanKochi-Umajimura Yuzu Health Research Course, Kochi Medical School, Kochi University, Nankoku 783-8505, JapanUmajimura Agricultural Cooperative, Kochi 781-6201, JapanKochi-Umajimura Yuzu Health Research Course, Kochi Medical School, Kochi University, Nankoku 783-8505, JapanLong-term oral ingestion of unheated yuzu seed oil in humans reduces lipid peroxides in the blood. Moreover, yuzu seed oil contains limonin, which can induce antioxidant and anti-inflammatory effects by activating the transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2). Previously, Nrf2 has been shown to reduce atopic dermatitis (AD). Therefore, we hypothesized that ingesting unheated yuzu seed oil can regulate AD through Nrf2. An AD model was established using NC/Nga mice through repeated local exposure to mite antigens. Unheated and purified yuzu seed oil (100 µL/mice) or water (control, 100 µL/mice) was administered orally once a day using a gastric cannula for rodents for 28 days. On day 28, mice in the unheated yuzu seed oil group exhibited significantly lower clinical skin severity scores and ear thickness than those in the purified yuzu seed oil and water groups. Serum histamine levels remained unaltered among the three AD-induced groups. Serum <i>Dermatophagoides farina</i> body (Dfb)-specific immunoglobulin E (IgE) levels were significantly lower in the unheated yuzu seed oil group. Oral ingestion of yuzu seed oil in NC/Nga AD model mice significantly suppressed dermatitis deterioration and decreased serum IgE levels. Clinical trials (<i>n</i> = 41) have already confirmed that unheated yuzu oil is safe for long-term intake, further suggesting its potential use in improving AD symptoms.https://www.mdpi.com/1422-0067/25/5/2689yuzu seed oillimoninatopic dermatitisNrf2antioxidant effect |
| spellingShingle | Kimito Asano Yoshiya Watanabe Mio Miyamoto Mochifumi Toutani Shunji Mizobuchi Oral Ingestion of Yuzu Seed Oil Suppresses the Development of Atopic Dermatitis-like Skin Lesions in NC/Nga Mice International Journal of Molecular Sciences yuzu seed oil limonin atopic dermatitis Nrf2 antioxidant effect |
| title | Oral Ingestion of Yuzu Seed Oil Suppresses the Development of Atopic Dermatitis-like Skin Lesions in NC/Nga Mice |
| title_full | Oral Ingestion of Yuzu Seed Oil Suppresses the Development of Atopic Dermatitis-like Skin Lesions in NC/Nga Mice |
| title_fullStr | Oral Ingestion of Yuzu Seed Oil Suppresses the Development of Atopic Dermatitis-like Skin Lesions in NC/Nga Mice |
| title_full_unstemmed | Oral Ingestion of Yuzu Seed Oil Suppresses the Development of Atopic Dermatitis-like Skin Lesions in NC/Nga Mice |
| title_short | Oral Ingestion of Yuzu Seed Oil Suppresses the Development of Atopic Dermatitis-like Skin Lesions in NC/Nga Mice |
| title_sort | oral ingestion of yuzu seed oil suppresses the development of atopic dermatitis like skin lesions in nc nga mice |
| topic | yuzu seed oil limonin atopic dermatitis Nrf2 antioxidant effect |
| url | https://www.mdpi.com/1422-0067/25/5/2689 |
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