Effects of Pyrroloquinoline Quinone (PQQ) on Ischemia-Reperfusion Injury in Rat Ovaries: Histological and Biochemical Assessments
Objective: To evaluate effects of pyrroloquinoline quinone (PQQ) on ischemia-reperfusion injury using a rat ovary model.Method: Thirty healthy female Wistar rats with 250g were randomized into five experimental groups (n = 6): Group SHAM: The rats underwent only laparotomy. Group Ischemia: A 3- hour...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2019-01-01
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| Series: | Bulletin of Emergency and Trauma |
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| Online Access: | http://beat.sums.ac.ir/article_44471_438d83f33a1002671a10cda8c15c7695.pdf |
| _version_ | 1818821350739935232 |
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| author | Pegah Parhizkar Rahim Mohammadi Rasoul Shahrouz Vahid Mohammadi |
| author_facet | Pegah Parhizkar Rahim Mohammadi Rasoul Shahrouz Vahid Mohammadi |
| author_sort | Pegah Parhizkar |
| collection | DOAJ |
| description | Objective: To evaluate effects of pyrroloquinoline quinone (PQQ) on ischemia-reperfusion injury using a rat ovary model.Method: Thirty healthy female Wistar rats with 250g were randomized into five experimental groups (n = 6): Group SHAM: The rats underwent only laparotomy. Group Ischemia: A 3- hour ischemia only. Group I/R: A 3-hour ischemia and a 3-hour reperfusion. 30 min before termination of reperfusion 20 µL soybeen oil (Solvent of PQQ) was administered. Group I/PQQ: A 3-hour ischemia only and 20 µL (10 mg/kg) intraperitoneal administration (IP) of PQQ 2.5 hours after induction of ischemia. Group I/R/PQQ: A 3-hour ischemia, a 3-hour reperfusion and 20 µL (10 mg/kg) IP of PQQ 2.5 hours after induction of ischemia. Results: Animals treated with PQQ showed significantly ameliorated development of ischemia and reperfusion tissue injury compared to those of other groups (p=0.001). The significant higher values of SOD, GPO and GST were observed in I/R/PQQ animals compared to those of other groups (p=0.001). Damage indicator (MDA) was significantly lower in I/R/PQQ animal compared to those of other groups (p=0.001). Conclusion: Intraperitoneal administration of PQQ could be helpful in minimizing ischemia-reperfusion injury in ovarian tissue exposed to ischemia. |
| first_indexed | 2024-12-18T23:06:48Z |
| format | Article |
| id | doaj.art-d6cf975bfae64e1ab623ed8140d495e5 |
| institution | Directory Open Access Journal |
| issn | 2322-2522 2322-3960 |
| language | English |
| last_indexed | 2024-12-18T23:06:48Z |
| publishDate | 2019-01-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Bulletin of Emergency and Trauma |
| spelling | doaj.art-d6cf975bfae64e1ab623ed8140d495e52022-12-21T20:48:25ZengShiraz University of Medical SciencesBulletin of Emergency and Trauma2322-25222322-39602019-01-017Issue 1354010.29252/beat-07010544471Effects of Pyrroloquinoline Quinone (PQQ) on Ischemia-Reperfusion Injury in Rat Ovaries: Histological and Biochemical AssessmentsPegah Parhizkar0Rahim Mohammadi1Rasoul Shahrouz2Vahid Mohammadi3Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia University, Urmia, IranDepartment of Internal medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, IranObjective: To evaluate effects of pyrroloquinoline quinone (PQQ) on ischemia-reperfusion injury using a rat ovary model.Method: Thirty healthy female Wistar rats with 250g were randomized into five experimental groups (n = 6): Group SHAM: The rats underwent only laparotomy. Group Ischemia: A 3- hour ischemia only. Group I/R: A 3-hour ischemia and a 3-hour reperfusion. 30 min before termination of reperfusion 20 µL soybeen oil (Solvent of PQQ) was administered. Group I/PQQ: A 3-hour ischemia only and 20 µL (10 mg/kg) intraperitoneal administration (IP) of PQQ 2.5 hours after induction of ischemia. Group I/R/PQQ: A 3-hour ischemia, a 3-hour reperfusion and 20 µL (10 mg/kg) IP of PQQ 2.5 hours after induction of ischemia. Results: Animals treated with PQQ showed significantly ameliorated development of ischemia and reperfusion tissue injury compared to those of other groups (p=0.001). The significant higher values of SOD, GPO and GST were observed in I/R/PQQ animals compared to those of other groups (p=0.001). Damage indicator (MDA) was significantly lower in I/R/PQQ animal compared to those of other groups (p=0.001). Conclusion: Intraperitoneal administration of PQQ could be helpful in minimizing ischemia-reperfusion injury in ovarian tissue exposed to ischemia.http://beat.sums.ac.ir/article_44471_438d83f33a1002671a10cda8c15c7695.pdfIschemia-reperfusionPyrroloquinoline Quinone (PQQ)IntraperitonealOvary |
| spellingShingle | Pegah Parhizkar Rahim Mohammadi Rasoul Shahrouz Vahid Mohammadi Effects of Pyrroloquinoline Quinone (PQQ) on Ischemia-Reperfusion Injury in Rat Ovaries: Histological and Biochemical Assessments Bulletin of Emergency and Trauma Ischemia-reperfusion Pyrroloquinoline Quinone (PQQ) Intraperitoneal Ovary |
| title | Effects of Pyrroloquinoline Quinone (PQQ) on Ischemia-Reperfusion Injury in Rat Ovaries: Histological and Biochemical Assessments |
| title_full | Effects of Pyrroloquinoline Quinone (PQQ) on Ischemia-Reperfusion Injury in Rat Ovaries: Histological and Biochemical Assessments |
| title_fullStr | Effects of Pyrroloquinoline Quinone (PQQ) on Ischemia-Reperfusion Injury in Rat Ovaries: Histological and Biochemical Assessments |
| title_full_unstemmed | Effects of Pyrroloquinoline Quinone (PQQ) on Ischemia-Reperfusion Injury in Rat Ovaries: Histological and Biochemical Assessments |
| title_short | Effects of Pyrroloquinoline Quinone (PQQ) on Ischemia-Reperfusion Injury in Rat Ovaries: Histological and Biochemical Assessments |
| title_sort | effects of pyrroloquinoline quinone pqq on ischemia reperfusion injury in rat ovaries histological and biochemical assessments |
| topic | Ischemia-reperfusion Pyrroloquinoline Quinone (PQQ) Intraperitoneal Ovary |
| url | http://beat.sums.ac.ir/article_44471_438d83f33a1002671a10cda8c15c7695.pdf |
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