The effects of high dose of two manganese supplements (organic and inorganic) on the rumen microbial ecosystem.
Little is known about the effects of the high dose and types of manganese supplements on rumen environment at manganese intake level close above the limit of 150 mg/kg of dry feed matter. The effects of high dose of two manganese supplements (organic and inorganic) on rumen microbial ecosystem after...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5764370?pdf=render |
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| author | Svetlana Kišidayová Peter Pristaš Michaela Zimovčáková Monika Blanár Wencelová Lucia Homol'ová Katarína Mihaliková Klaudia Čobanová Ľubomíra Grešáková Zora Váradyová |
| author_facet | Svetlana Kišidayová Peter Pristaš Michaela Zimovčáková Monika Blanár Wencelová Lucia Homol'ová Katarína Mihaliková Klaudia Čobanová Ľubomíra Grešáková Zora Váradyová |
| author_sort | Svetlana Kišidayová |
| collection | DOAJ |
| description | Little is known about the effects of the high dose and types of manganese supplements on rumen environment at manganese intake level close above the limit of 150 mg/kg of dry feed matter. The effects of high dose of two manganese supplements (organic and inorganic) on rumen microbial ecosystem after four months of treatment of 18 lambs divided into three treatment groups were studied. We examined the enzyme activities (α-amylase, xylanase, and carboxymethyl cellulase), total and differential microscopic counts of rumen ciliates, total microscopic counts of bacteria, and fingerprinting pattern of the eubacterial and ciliates population analyzed by PCR-DGGE. Lambs were fed a basal diet with a basal Mn content (34.3 mg/kg dry matter; control) and supplemented either with inorganic manganous sulfate or organic Mn-chelate hydrate (daily 182.7, 184 mg/kg dry matter of feed, respectively). Basal diet, offered twice daily, consisted of ground barley and hay (268 and 732 g/kg dry matter per animal and day). The rumens of the lambs harbored ciliates of the genera of Entodinium, Epidinium, Diplodinium, Eudiplodinium, Dasytricha, and Isotricha. No significant differences between treatment groups were observed in the total ciliate number, the number of ciliates at the genus level, as well as the total number of bacteria. Organic Mn did decrease the species richness and diversity of the eubacterial population examined by PCR-DGGE. No effects of type of Mn supplement on the enzyme activities were observed. In comparison to the control, α-amylase specific activities were decreased and carboxymethyl-cellulase specific activities were increased by the Mn supplements. Xylanase activities were not influenced. In conclusion, our results suggested that the intake of tested inorganic and organic manganese supplements in excess may affect the specific groups of eubacteria. More studies on intake of Mn supplements at a level close to the limit can reveal if the changes in microbial population impact remarkably the other rumen enzymatic activities. |
| first_indexed | 2024-12-11T08:44:50Z |
| format | Article |
| id | doaj.art-d6d0c3f01eb0426095f551adea4b01a4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T08:44:50Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d6d0c3f01eb0426095f551adea4b01a42022-12-22T01:14:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01131e019115810.1371/journal.pone.0191158The effects of high dose of two manganese supplements (organic and inorganic) on the rumen microbial ecosystem.Svetlana KišidayováPeter PristašMichaela ZimovčákováMonika Blanár WencelováLucia Homol'ováKatarína MihalikováKlaudia ČobanováĽubomíra GrešákováZora VáradyováLittle is known about the effects of the high dose and types of manganese supplements on rumen environment at manganese intake level close above the limit of 150 mg/kg of dry feed matter. The effects of high dose of two manganese supplements (organic and inorganic) on rumen microbial ecosystem after four months of treatment of 18 lambs divided into three treatment groups were studied. We examined the enzyme activities (α-amylase, xylanase, and carboxymethyl cellulase), total and differential microscopic counts of rumen ciliates, total microscopic counts of bacteria, and fingerprinting pattern of the eubacterial and ciliates population analyzed by PCR-DGGE. Lambs were fed a basal diet with a basal Mn content (34.3 mg/kg dry matter; control) and supplemented either with inorganic manganous sulfate or organic Mn-chelate hydrate (daily 182.7, 184 mg/kg dry matter of feed, respectively). Basal diet, offered twice daily, consisted of ground barley and hay (268 and 732 g/kg dry matter per animal and day). The rumens of the lambs harbored ciliates of the genera of Entodinium, Epidinium, Diplodinium, Eudiplodinium, Dasytricha, and Isotricha. No significant differences between treatment groups were observed in the total ciliate number, the number of ciliates at the genus level, as well as the total number of bacteria. Organic Mn did decrease the species richness and diversity of the eubacterial population examined by PCR-DGGE. No effects of type of Mn supplement on the enzyme activities were observed. In comparison to the control, α-amylase specific activities were decreased and carboxymethyl-cellulase specific activities were increased by the Mn supplements. Xylanase activities were not influenced. In conclusion, our results suggested that the intake of tested inorganic and organic manganese supplements in excess may affect the specific groups of eubacteria. More studies on intake of Mn supplements at a level close to the limit can reveal if the changes in microbial population impact remarkably the other rumen enzymatic activities.http://europepmc.org/articles/PMC5764370?pdf=render |
| spellingShingle | Svetlana Kišidayová Peter Pristaš Michaela Zimovčáková Monika Blanár Wencelová Lucia Homol'ová Katarína Mihaliková Klaudia Čobanová Ľubomíra Grešáková Zora Váradyová The effects of high dose of two manganese supplements (organic and inorganic) on the rumen microbial ecosystem. PLoS ONE |
| title | The effects of high dose of two manganese supplements (organic and inorganic) on the rumen microbial ecosystem. |
| title_full | The effects of high dose of two manganese supplements (organic and inorganic) on the rumen microbial ecosystem. |
| title_fullStr | The effects of high dose of two manganese supplements (organic and inorganic) on the rumen microbial ecosystem. |
| title_full_unstemmed | The effects of high dose of two manganese supplements (organic and inorganic) on the rumen microbial ecosystem. |
| title_short | The effects of high dose of two manganese supplements (organic and inorganic) on the rumen microbial ecosystem. |
| title_sort | effects of high dose of two manganese supplements organic and inorganic on the rumen microbial ecosystem |
| url | http://europepmc.org/articles/PMC5764370?pdf=render |
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