Mining the Roles of Wheat (Triticum aestivum) SnRK Genes in Biotic and Abiotic Responses
Sucrose non-fermenting-1-related protein kinases (SnRKs) play vital roles in plant growth and stress responses. However, little is known about the SnRK functions in wheat. In this study, 149 TaSnRKs (wheat SnRKs) were identified and were divided into three subfamilies. A combination of public transc...
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| Format: | Article |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.934226/full |
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| author | Baihui Jiang Yike Liu Hongli Niu Yiqin He Yiqin He Dongfang Ma Dongfang Ma Yan Li Yan Li |
| author_facet | Baihui Jiang Yike Liu Hongli Niu Yiqin He Yiqin He Dongfang Ma Dongfang Ma Yan Li Yan Li |
| author_sort | Baihui Jiang |
| collection | DOAJ |
| description | Sucrose non-fermenting-1-related protein kinases (SnRKs) play vital roles in plant growth and stress responses. However, little is known about the SnRK functions in wheat. In this study, 149 TaSnRKs (wheat SnRKs) were identified and were divided into three subfamilies. A combination of public transcriptome data and real-time reverse transcription-polymerase chain reaction (qRT-PCR) analysis revealed the distinct expression patterns of TaSnRKs under various abiotic and biotic stresses. TaSnRK2.4-B, a member of SnRK2s, has different expression patterns under polyethylene glycol (PEG), sodium chloride (NaCl) treatment, and high concentrations of abscisic acid (ABA) application. Yeast two-hybrid assay indicated that TaSnRK2.4-B could interact with the SnRK2-interacting calcium sensor (SCS) in wheat and play a role in the ABA-dependent pathway. Moreover, TaSnRK2.4-B might be a negative regulator in wheat against pathogen infection. The present study provides valuable information for understanding the functions of the TaSnRK family and provides recommendations for future genetic improvement in wheat stress resistance. |
| first_indexed | 2024-04-12T12:25:52Z |
| format | Article |
| id | doaj.art-d6d385bee9804f0c86a459788ee0aed1 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T12:25:52Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-d6d385bee9804f0c86a459788ee0aed12022-12-22T03:33:10ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-06-011310.3389/fpls.2022.934226934226Mining the Roles of Wheat (Triticum aestivum) SnRK Genes in Biotic and Abiotic ResponsesBaihui Jiang0Yike Liu1Hongli Niu2Yiqin He3Yiqin He4Dongfang Ma5Dongfang Ma6Yan Li7Yan Li8Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/College of Agriculture, Yangtze University, Jingzhou, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences/Wheat Disease Biology Research Station for Central China, Wuhan, ChinaEngineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/College of Agriculture, Yangtze University, Jingzhou, ChinaEngineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/College of Agriculture, Yangtze University, Jingzhou, ChinaLonggan Lake National Nature Reserve Authority of Hubei, Huanggang, ChinaEngineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/College of Agriculture, Yangtze University, Jingzhou, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences/Wheat Disease Biology Research Station for Central China, Wuhan, ChinaEngineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/College of Agriculture, Yangtze University, Jingzhou, ChinaHubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences/Wheat Disease Biology Research Station for Central China, Wuhan, ChinaSucrose non-fermenting-1-related protein kinases (SnRKs) play vital roles in plant growth and stress responses. However, little is known about the SnRK functions in wheat. In this study, 149 TaSnRKs (wheat SnRKs) were identified and were divided into three subfamilies. A combination of public transcriptome data and real-time reverse transcription-polymerase chain reaction (qRT-PCR) analysis revealed the distinct expression patterns of TaSnRKs under various abiotic and biotic stresses. TaSnRK2.4-B, a member of SnRK2s, has different expression patterns under polyethylene glycol (PEG), sodium chloride (NaCl) treatment, and high concentrations of abscisic acid (ABA) application. Yeast two-hybrid assay indicated that TaSnRK2.4-B could interact with the SnRK2-interacting calcium sensor (SCS) in wheat and play a role in the ABA-dependent pathway. Moreover, TaSnRK2.4-B might be a negative regulator in wheat against pathogen infection. The present study provides valuable information for understanding the functions of the TaSnRK family and provides recommendations for future genetic improvement in wheat stress resistance.https://www.frontiersin.org/articles/10.3389/fpls.2022.934226/fullTaSnRKbiotic and abiotic stressesqRT-PCRTaSnRK2.4-Bnegative regulator |
| spellingShingle | Baihui Jiang Yike Liu Hongli Niu Yiqin He Yiqin He Dongfang Ma Dongfang Ma Yan Li Yan Li Mining the Roles of Wheat (Triticum aestivum) SnRK Genes in Biotic and Abiotic Responses Frontiers in Plant Science TaSnRK biotic and abiotic stresses qRT-PCR TaSnRK2.4-B negative regulator |
| title | Mining the Roles of Wheat (Triticum aestivum) SnRK Genes in Biotic and Abiotic Responses |
| title_full | Mining the Roles of Wheat (Triticum aestivum) SnRK Genes in Biotic and Abiotic Responses |
| title_fullStr | Mining the Roles of Wheat (Triticum aestivum) SnRK Genes in Biotic and Abiotic Responses |
| title_full_unstemmed | Mining the Roles of Wheat (Triticum aestivum) SnRK Genes in Biotic and Abiotic Responses |
| title_short | Mining the Roles of Wheat (Triticum aestivum) SnRK Genes in Biotic and Abiotic Responses |
| title_sort | mining the roles of wheat triticum aestivum snrk genes in biotic and abiotic responses |
| topic | TaSnRK biotic and abiotic stresses qRT-PCR TaSnRK2.4-B negative regulator |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.934226/full |
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