The Essential Genome of <italic toggle="yes">Escherichia coli</italic> K-12

ABSTRACT Transposon-directed insertion site sequencing (TraDIS) is a high-throughput method coupling transposon mutagenesis with short-fragment DNA sequencing. It is commonly used to identify essential genes. Single gene deletion libraries are considered the gold standard for identifying essential g...

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Bibliographic Details
Main Authors: Emily C. A. Goodall, Ashley Robinson, Iain G. Johnston, Sara Jabbari, Keith A. Turner, Adam F. Cunningham, Peter A. Lund, Jeffrey A. Cole, Ian R. Henderson
Format: Article
Language:English
Published: American Society for Microbiology 2018-03-01
Series:mBio
Subjects:
Online Access:https://journals.asm.org/doi/10.1128/mBio.02096-17