Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo
Summary Cell migration is an important biological process which has been intensively studied in the past decades. Numerous techniques, mainly involving two-dimensional cell culture systems, have contributed to dissecting the essential mechanisms underlying this process. However, the development of t...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2013-06-01
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| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/2/8/795 |
| _version_ | 1818672984512004096 |
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| author | Carolina G. A. Moreira Antonio Jacinto Soren Prag |
| author_facet | Carolina G. A. Moreira Antonio Jacinto Soren Prag |
| author_sort | Carolina G. A. Moreira |
| collection | DOAJ |
| description | Summary
Cell migration is an important biological process which has been intensively studied in the past decades. Numerous techniques, mainly involving two-dimensional cell culture systems, have contributed to dissecting the essential mechanisms underlying this process. However, the development of three-dimensional cell culture and in vivo systems has shown some differences with what was previously believed to be well-established cell migration mechanisms, suggesting that two-dimensional cell motility would be a poor predictor of in vivo behaviour. Drosophila is a widely recognized model organism to study developmental and homeostatic processes and has been widely used to investigate cell migration. Here, we focus on the migration of small groups of pupal hemocytes that accumulate during larval stages in dorsal patches. We show that integrins, and other known nascent adhesion-related proteins such as Rhea and Fermitin 1, are crucial for this process and that their depletion does not affect polarization in response to environmental cues. We also present evidence for the importance of adhesion maturation-related proteins in hemocyte migration, namely Zyxin. Zyxin depletion in hemocytes leads to a significant increase of cell speed without affecting their response to a chemotactic cue. This is the first report of a systematic analysis using Drosophila melanogaster hemocytes to study adhesion-related proteins and their function in cell migration in vivo. Our data point to mechanisms of cell migration similar to those described in three-dimensional in vitro systems and other in vivo model organisms. |
| first_indexed | 2024-12-17T07:48:35Z |
| format | Article |
| id | doaj.art-d6d709907943469794802c205dd9b348 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-17T07:48:35Z |
| publishDate | 2013-06-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-d6d709907943469794802c205dd9b3482022-12-21T21:57:55ZengThe Company of BiologistsBiology Open2046-63902013-06-012879580110.1242/bio.2013456420134564Drosophila integrin adhesion complexes are essential for hemocyte migration in vivoCarolina G. A. Moreira0Antonio Jacinto1Soren Prag2 Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal CEDOC, Faculdade de Ciências Médicas, Universidade Nova de Lisboa, 1169-056 Lisboa, Portugal Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal Summary Cell migration is an important biological process which has been intensively studied in the past decades. Numerous techniques, mainly involving two-dimensional cell culture systems, have contributed to dissecting the essential mechanisms underlying this process. However, the development of three-dimensional cell culture and in vivo systems has shown some differences with what was previously believed to be well-established cell migration mechanisms, suggesting that two-dimensional cell motility would be a poor predictor of in vivo behaviour. Drosophila is a widely recognized model organism to study developmental and homeostatic processes and has been widely used to investigate cell migration. Here, we focus on the migration of small groups of pupal hemocytes that accumulate during larval stages in dorsal patches. We show that integrins, and other known nascent adhesion-related proteins such as Rhea and Fermitin 1, are crucial for this process and that their depletion does not affect polarization in response to environmental cues. We also present evidence for the importance of adhesion maturation-related proteins in hemocyte migration, namely Zyxin. Zyxin depletion in hemocytes leads to a significant increase of cell speed without affecting their response to a chemotactic cue. This is the first report of a systematic analysis using Drosophila melanogaster hemocytes to study adhesion-related proteins and their function in cell migration in vivo. Our data point to mechanisms of cell migration similar to those described in three-dimensional in vitro systems and other in vivo model organisms.http://bio.biologists.org/content/2/8/795DrosophilaHemocyteIntegrinMigration |
| spellingShingle | Carolina G. A. Moreira Antonio Jacinto Soren Prag Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo Biology Open Drosophila Hemocyte Integrin Migration |
| title | Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo |
| title_full | Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo |
| title_fullStr | Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo |
| title_full_unstemmed | Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo |
| title_short | Drosophila integrin adhesion complexes are essential for hemocyte migration in vivo |
| title_sort | drosophila integrin adhesion complexes are essential for hemocyte migration in vivo |
| topic | Drosophila Hemocyte Integrin Migration |
| url | http://bio.biologists.org/content/2/8/795 |
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