Evaluation of the Stability of Bacteriophages in Different Solutions Suitable for the Production of Magistral Preparations in Belgium
In Belgium, the incorporation of phages into magistral preparations for human application has been permitted since 2018. The stability of such preparations is of high importance to guarantee quality and efficacy throughout treatments. We evaluated the ability to preserve infectivity of four differen...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/13/5/865 |
| _version_ | 1797534897007493120 |
|---|---|
| author | Hans Duyvejonck Maya Merabishvili Mario Vaneechoutte Steven de Soir Rosanna Wright Ville-Petri Friman Gilbert Verbeken Daniel De Vos Jean-Paul Pirnay Els Van Mechelen Stefan J. T. Vermeulen |
| author_facet | Hans Duyvejonck Maya Merabishvili Mario Vaneechoutte Steven de Soir Rosanna Wright Ville-Petri Friman Gilbert Verbeken Daniel De Vos Jean-Paul Pirnay Els Van Mechelen Stefan J. T. Vermeulen |
| author_sort | Hans Duyvejonck |
| collection | DOAJ |
| description | In Belgium, the incorporation of phages into magistral preparations for human application has been permitted since 2018. The stability of such preparations is of high importance to guarantee quality and efficacy throughout treatments. We evaluated the ability to preserve infectivity of four different phages active against three different bacterial species in five different buffer and infusion solutions commonly used in medicine and biotechnological manufacturing processes, at two different concentrations (9 and 7 log pfu/mL), stored at 4 °C. DPBS without Ca<sup>2+</sup> and Mg<sup>2+</sup> was found to be the best option, compared to the other solutions. Suspensions with phage concentrations of 7 log pfu/mL were unsuited as their activity dropped below the effective therapeutic dose (6–9 log pfu/mL), even after one week of storage at 4 °C. Strong variability between phages was observed, with <i>Acinetobacter baumannii</i> phage Acibel004 being stable in four out of five different solutions. We also studied the long term storage of lyophilized staphylococcal phage ISP, and found that the titer could be preserved during a period of almost 8 years when sucrose and trehalose were used as stabilizers. After rehydration of the lyophilized ISP phage in saline, the phage solutions remained stable at 4 °C during a period of 126 days. |
| first_indexed | 2024-03-10T11:36:58Z |
| format | Article |
| id | doaj.art-d6db5cbf7d9e489a9d87983fecf34633 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T11:36:58Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-d6db5cbf7d9e489a9d87983fecf346332023-11-21T18:49:52ZengMDPI AGViruses1999-49152021-05-0113586510.3390/v13050865Evaluation of the Stability of Bacteriophages in Different Solutions Suitable for the Production of Magistral Preparations in BelgiumHans Duyvejonck0Maya Merabishvili1Mario Vaneechoutte2Steven de Soir3Rosanna Wright4Ville-Petri Friman5Gilbert Verbeken6Daniel De Vos7Jean-Paul Pirnay8Els Van Mechelen9Stefan J. T. Vermeulen10Research Center Health & Water Technology, University College Ghent, Keramiekstraat 80, B-9000 Gent, BelgiumLaboratory Bacteriology Research, Faculty of Medicine & Health Sciences, Ghent University, C. Heymanslaan 10, B-9000 Gent, BelgiumLaboratory Bacteriology Research, Faculty of Medicine & Health Sciences, Ghent University, C. Heymanslaan 10, B-9000 Gent, BelgiumLaboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Bruynstraat 1, 1120 Brussel, BelgiumDepartment of Biology, University of York, Wentworth Way, York YO10 5DD, UKDepartment of Biology, University of York, Wentworth Way, York YO10 5DD, UKLaboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Bruynstraat 1, 1120 Brussel, BelgiumLaboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Bruynstraat 1, 1120 Brussel, BelgiumLaboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Bruynstraat 1, 1120 Brussel, BelgiumResearch Center Health & Water Technology, University College Ghent, Keramiekstraat 80, B-9000 Gent, BelgiumResearch Center Health & Water Technology, University College Ghent, Keramiekstraat 80, B-9000 Gent, BelgiumIn Belgium, the incorporation of phages into magistral preparations for human application has been permitted since 2018. The stability of such preparations is of high importance to guarantee quality and efficacy throughout treatments. We evaluated the ability to preserve infectivity of four different phages active against three different bacterial species in five different buffer and infusion solutions commonly used in medicine and biotechnological manufacturing processes, at two different concentrations (9 and 7 log pfu/mL), stored at 4 °C. DPBS without Ca<sup>2+</sup> and Mg<sup>2+</sup> was found to be the best option, compared to the other solutions. Suspensions with phage concentrations of 7 log pfu/mL were unsuited as their activity dropped below the effective therapeutic dose (6–9 log pfu/mL), even after one week of storage at 4 °C. Strong variability between phages was observed, with <i>Acinetobacter baumannii</i> phage Acibel004 being stable in four out of five different solutions. We also studied the long term storage of lyophilized staphylococcal phage ISP, and found that the titer could be preserved during a period of almost 8 years when sucrose and trehalose were used as stabilizers. After rehydration of the lyophilized ISP phage in saline, the phage solutions remained stable at 4 °C during a period of 126 days.https://www.mdpi.com/1999-4915/13/5/865phageAPImagistral preparationbuffersinfusion solutionsinfectivity |
| spellingShingle | Hans Duyvejonck Maya Merabishvili Mario Vaneechoutte Steven de Soir Rosanna Wright Ville-Petri Friman Gilbert Verbeken Daniel De Vos Jean-Paul Pirnay Els Van Mechelen Stefan J. T. Vermeulen Evaluation of the Stability of Bacteriophages in Different Solutions Suitable for the Production of Magistral Preparations in Belgium Viruses phage API magistral preparation buffers infusion solutions infectivity |
| title | Evaluation of the Stability of Bacteriophages in Different Solutions Suitable for the Production of Magistral Preparations in Belgium |
| title_full | Evaluation of the Stability of Bacteriophages in Different Solutions Suitable for the Production of Magistral Preparations in Belgium |
| title_fullStr | Evaluation of the Stability of Bacteriophages in Different Solutions Suitable for the Production of Magistral Preparations in Belgium |
| title_full_unstemmed | Evaluation of the Stability of Bacteriophages in Different Solutions Suitable for the Production of Magistral Preparations in Belgium |
| title_short | Evaluation of the Stability of Bacteriophages in Different Solutions Suitable for the Production of Magistral Preparations in Belgium |
| title_sort | evaluation of the stability of bacteriophages in different solutions suitable for the production of magistral preparations in belgium |
| topic | phage API magistral preparation buffers infusion solutions infectivity |
| url | https://www.mdpi.com/1999-4915/13/5/865 |
| work_keys_str_mv | AT hansduyvejonck evaluationofthestabilityofbacteriophagesindifferentsolutionssuitablefortheproductionofmagistralpreparationsinbelgium AT mayamerabishvili evaluationofthestabilityofbacteriophagesindifferentsolutionssuitablefortheproductionofmagistralpreparationsinbelgium AT mariovaneechoutte evaluationofthestabilityofbacteriophagesindifferentsolutionssuitablefortheproductionofmagistralpreparationsinbelgium AT stevendesoir evaluationofthestabilityofbacteriophagesindifferentsolutionssuitablefortheproductionofmagistralpreparationsinbelgium AT rosannawright evaluationofthestabilityofbacteriophagesindifferentsolutionssuitablefortheproductionofmagistralpreparationsinbelgium AT villepetrifriman evaluationofthestabilityofbacteriophagesindifferentsolutionssuitablefortheproductionofmagistralpreparationsinbelgium AT gilbertverbeken evaluationofthestabilityofbacteriophagesindifferentsolutionssuitablefortheproductionofmagistralpreparationsinbelgium AT danieldevos evaluationofthestabilityofbacteriophagesindifferentsolutionssuitablefortheproductionofmagistralpreparationsinbelgium AT jeanpaulpirnay evaluationofthestabilityofbacteriophagesindifferentsolutionssuitablefortheproductionofmagistralpreparationsinbelgium AT elsvanmechelen evaluationofthestabilityofbacteriophagesindifferentsolutionssuitablefortheproductionofmagistralpreparationsinbelgium AT stefanjtvermeulen evaluationofthestabilityofbacteriophagesindifferentsolutionssuitablefortheproductionofmagistralpreparationsinbelgium |