The interplay of GTP-binding protein AGB1 with ER stress sensors IRE1a and IRE1b modulates Arabidopsis unfolded protein response and bacterial immunity
In eukaryotic cells, the accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) results in ER stress that induces a cascade of reactions called the unfolded protein response (UPR). In Arabidopsis, the most conserved UPR sensor, Inositol-requiring enzyme 1 (IRE1), responds t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Plant Signaling & Behavior |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592324.2021.2018857 |
| _version_ | 1797351993430245376 |
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| author | Taiaba Afrin Caitlin N. Costello Amber N. Monella Camilla J. Kørner Karolina M. Pajerowska-Mukhtar |
| author_facet | Taiaba Afrin Caitlin N. Costello Amber N. Monella Camilla J. Kørner Karolina M. Pajerowska-Mukhtar |
| author_sort | Taiaba Afrin |
| collection | DOAJ |
| description | In eukaryotic cells, the accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) results in ER stress that induces a cascade of reactions called the unfolded protein response (UPR). In Arabidopsis, the most conserved UPR sensor, Inositol-requiring enzyme 1 (IRE1), responds to both abiotic- and biotic-induced ER stress. Guanine nucleotide-binding proteins (G proteins) constitute another universal and conserved family of signal transducers that have been extensively investigated due to their ubiquitous presence and diverse nature of action. Arabidopsis GTP-binding protein β1 (AGB1) is the only G-protein β-subunit encoded by the Arabidopsis genome that is involved in numerous signaling pathways. Mounting evidence suggests the existence of a crosstalk between IRE1 and G protein signaling during ER stress. AGB1 has previously been shown to control a distinct UPR pathway independently of IRE1 when treated with an ER stress inducer tunicamycin. Our results obtained with combinatorial knockout mutants support the hypothesis that both IRE1 and AGB1 synergistically contribute to ER stress responses chemically induced by dithiothreitol (DTT) as well as to the immune responses against a phytopathogenic bacterium Pseudomonas syringae pv. tomato strain DC3000. Our study highlights the crosstalk between the plant UPR transducers under abiotic and biotic stress. |
| first_indexed | 2024-03-08T13:08:46Z |
| format | Article |
| id | doaj.art-d6dc49fc0e834951ae861d121bc2e06e |
| institution | Directory Open Access Journal |
| issn | 1559-2316 1559-2324 |
| language | English |
| last_indexed | 2024-03-08T13:08:46Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Signaling & Behavior |
| spelling | doaj.art-d6dc49fc0e834951ae861d121bc2e06e2024-01-18T15:58:21ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242022-12-0117110.1080/15592324.2021.20188572018857The interplay of GTP-binding protein AGB1 with ER stress sensors IRE1a and IRE1b modulates Arabidopsis unfolded protein response and bacterial immunityTaiaba Afrin0Caitlin N. Costello1Amber N. Monella2Camilla J. Kørner3Karolina M. Pajerowska-Mukhtar4University of Alabama at Birmingham, 1300 University BlvdUniversity of Alabama at Birmingham, 1300 University BlvdUniversity of Alabama at Birmingham, 1300 University BlvdUniversity of Alabama at Birmingham, 1300 University BlvdUniversity of Alabama at Birmingham, 1300 University BlvdIn eukaryotic cells, the accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) results in ER stress that induces a cascade of reactions called the unfolded protein response (UPR). In Arabidopsis, the most conserved UPR sensor, Inositol-requiring enzyme 1 (IRE1), responds to both abiotic- and biotic-induced ER stress. Guanine nucleotide-binding proteins (G proteins) constitute another universal and conserved family of signal transducers that have been extensively investigated due to their ubiquitous presence and diverse nature of action. Arabidopsis GTP-binding protein β1 (AGB1) is the only G-protein β-subunit encoded by the Arabidopsis genome that is involved in numerous signaling pathways. Mounting evidence suggests the existence of a crosstalk between IRE1 and G protein signaling during ER stress. AGB1 has previously been shown to control a distinct UPR pathway independently of IRE1 when treated with an ER stress inducer tunicamycin. Our results obtained with combinatorial knockout mutants support the hypothesis that both IRE1 and AGB1 synergistically contribute to ER stress responses chemically induced by dithiothreitol (DTT) as well as to the immune responses against a phytopathogenic bacterium Pseudomonas syringae pv. tomato strain DC3000. Our study highlights the crosstalk between the plant UPR transducers under abiotic and biotic stress.http://dx.doi.org/10.1080/15592324.2021.2018857unfolded protein responseinositol-requiring enzyme 1gtp-binding protein b1pseudomonas syringae pv. tomato dc3000arabidopsis thaliana |
| spellingShingle | Taiaba Afrin Caitlin N. Costello Amber N. Monella Camilla J. Kørner Karolina M. Pajerowska-Mukhtar The interplay of GTP-binding protein AGB1 with ER stress sensors IRE1a and IRE1b modulates Arabidopsis unfolded protein response and bacterial immunity Plant Signaling & Behavior unfolded protein response inositol-requiring enzyme 1 gtp-binding protein b1 pseudomonas syringae pv. tomato dc3000 arabidopsis thaliana |
| title | The interplay of GTP-binding protein AGB1 with ER stress sensors IRE1a and IRE1b modulates Arabidopsis unfolded protein response and bacterial immunity |
| title_full | The interplay of GTP-binding protein AGB1 with ER stress sensors IRE1a and IRE1b modulates Arabidopsis unfolded protein response and bacterial immunity |
| title_fullStr | The interplay of GTP-binding protein AGB1 with ER stress sensors IRE1a and IRE1b modulates Arabidopsis unfolded protein response and bacterial immunity |
| title_full_unstemmed | The interplay of GTP-binding protein AGB1 with ER stress sensors IRE1a and IRE1b modulates Arabidopsis unfolded protein response and bacterial immunity |
| title_short | The interplay of GTP-binding protein AGB1 with ER stress sensors IRE1a and IRE1b modulates Arabidopsis unfolded protein response and bacterial immunity |
| title_sort | interplay of gtp binding protein agb1 with er stress sensors ire1a and ire1b modulates arabidopsis unfolded protein response and bacterial immunity |
| topic | unfolded protein response inositol-requiring enzyme 1 gtp-binding protein b1 pseudomonas syringae pv. tomato dc3000 arabidopsis thaliana |
| url | http://dx.doi.org/10.1080/15592324.2021.2018857 |
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