ADIBO-Based “Click” Chemistry for Diagnostic Peptide Micro-Array Fabrication: Physicochemical and Assay Characteristics

ADIBO-Based “Click” Chemistry for Diagnostic Peptide Micro-Array Fabrication: Physicochemical and Assay Characteristics

Several azide-derivatized and fluorescently-labeled peptides were immobilized on azadibenzocyclooctyne (ADIBO)-activated slide surfaces via a strain-promoted alkyne-azide cycloaddition (SPAAC) reaction revealing excellent immobilization kinetics, good spot homogeneities and reproducible fluorescence...

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Bibliographic Details
Main Authors: Marc E. Pfeifer, Philippe Passeraub, Vincent Cosandey, Roger Marti, Fabien Rebeaud, Denis Prim
Format: Article
Language:English
Published: MDPI AG 2013-08-01
Series:Molecules
Subjects:
peptide microarray
antibody diagnostics
SPAAC
ADIBO
kinetics
non-specific binding (NSB)
blocking reagents
Online Access:http://www.mdpi.com/1420-3049/18/8/9833

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