A recent study showed that miR-26a is downregulated in hepatocellular carcinoma tissues and that this downregulation is an independent predictor of survival. Interestingly, the same study also reported that miR-26a downregulation causes a concomitant elevation of IL-6 expression. Because miR-26a exp...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2013-01-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100032 |
| _version_ | 1818982948703043584 |
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| author | Yafei Zhang Bicheng Zhang Anran Zhang Xiaohua Li Jian Liu Jie Zhao Yong Zhao Jianfei Gao Dianchun Fang Zhiguo Rao |
| author_facet | Yafei Zhang Bicheng Zhang Anran Zhang Xiaohua Li Jian Liu Jie Zhao Yong Zhao Jianfei Gao Dianchun Fang Zhiguo Rao |
| author_sort | Yafei Zhang |
| collection | DOAJ |
| description | A recent study showed that miR-26a is downregulated in hepatocellular carcinoma tissues and that this downregulation is an independent predictor of survival. Interestingly, the same study also reported that miR-26a downregulation causes a concomitant elevation of IL-6 expression. Because miR-26a expression was found to be transcriptionally downregulated by oncogene c-Myc in various cancers, and the expression of c-Myc was increased by IL-6 stimulation, we hypothesized that IL-6 contributes to reduction of miR-26a in hepatocellular carcinoma. Serum IL-6 was measured by ELISA and miR-26a was detected by qRT-PCR. The data of 30 patients with hepatocellular carcinoma who had undergone surgical tumor resection revealed that serum IL-6 could be considered to be a predictor of survival up to 5 years for hepatocellular carcinoma patients (log-rank test, P < 0.05). We observed that the serum IL-6 concentration was inversely correlated with miR-26a expression in cancerous tissues (Pearson correlation test, r = -0.651, P < 0.01). Furthermore, by in vitro experiments with HepG2 cells, we showed that IL-6 stimulation can lead to miR-26a suppression via c-Myc activation, whereas in normal hepatocyte LO2 cells incubation with IL-6 had no significant effect on miR-26a expression. Taken together, these results indicate that miR-26a reduction in hepatocellular carcinoma might be due to IL-6 upregulation. |
| first_indexed | 2024-12-20T17:55:20Z |
| format | Article |
| id | doaj.art-d6dc8991287144d5a9c8d1cdc2437a3f |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-20T17:55:20Z |
| publishDate | 2013-01-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-d6dc8991287144d5a9c8d1cdc2437a3f2022-12-21T19:30:45ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2013-01-014613238Yafei ZhangBicheng ZhangAnran ZhangXiaohua LiJian LiuJie ZhaoYong ZhaoJianfei GaoDianchun FangZhiguo RaoA recent study showed that miR-26a is downregulated in hepatocellular carcinoma tissues and that this downregulation is an independent predictor of survival. Interestingly, the same study also reported that miR-26a downregulation causes a concomitant elevation of IL-6 expression. Because miR-26a expression was found to be transcriptionally downregulated by oncogene c-Myc in various cancers, and the expression of c-Myc was increased by IL-6 stimulation, we hypothesized that IL-6 contributes to reduction of miR-26a in hepatocellular carcinoma. Serum IL-6 was measured by ELISA and miR-26a was detected by qRT-PCR. The data of 30 patients with hepatocellular carcinoma who had undergone surgical tumor resection revealed that serum IL-6 could be considered to be a predictor of survival up to 5 years for hepatocellular carcinoma patients (log-rank test, P < 0.05). We observed that the serum IL-6 concentration was inversely correlated with miR-26a expression in cancerous tissues (Pearson correlation test, r = -0.651, P < 0.01). Furthermore, by in vitro experiments with HepG2 cells, we showed that IL-6 stimulation can lead to miR-26a suppression via c-Myc activation, whereas in normal hepatocyte LO2 cells incubation with IL-6 had no significant effect on miR-26a expression. Taken together, these results indicate that miR-26a reduction in hepatocellular carcinoma might be due to IL-6 upregulation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100032MiR-26aIL-6c-MycHepatocellular carcinomaCarcinoma prognosis |
| spellingShingle | Yafei Zhang Bicheng Zhang Anran Zhang Xiaohua Li Jian Liu Jie Zhao Yong Zhao Jianfei Gao Dianchun Fang Zhiguo Rao Brazilian Journal of Medical and Biological Research MiR-26a IL-6 c-Myc Hepatocellular carcinoma Carcinoma prognosis |
| topic | MiR-26a IL-6 c-Myc Hepatocellular carcinoma Carcinoma prognosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100032 |