Exploration of Novel Antibacterial and Anti-adhesive Formulations from three Chemically Characterized Essential oils: Optimization using experimental design methodology
In this study, experimental design approach coupled with MIC assay were used to determine the combined treatment effect of three Moroccan essential oils (EOs) derived from Ammi visnaga (AVEO), Mentha suaveolens (MSEO) and Lavandula stoechas (LSEO), against S. aureus, E. coli and P. aeruginosa, as we...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Scientific African |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468227623003824 |
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| author | Naoufal El Hachlafi Nesrine Benkhaira Nouhaila Zouine Mouhcine Fadil Mohamed Jeddi Samir Jeddi Rachid Flouchi Saad Ibnsouda Koraichi Kawtar Fikri-Benbrahim |
| author_facet | Naoufal El Hachlafi Nesrine Benkhaira Nouhaila Zouine Mouhcine Fadil Mohamed Jeddi Samir Jeddi Rachid Flouchi Saad Ibnsouda Koraichi Kawtar Fikri-Benbrahim |
| author_sort | Naoufal El Hachlafi |
| collection | DOAJ |
| description | In this study, experimental design approach coupled with MIC assay were used to determine the combined treatment effect of three Moroccan essential oils (EOs) derived from Ammi visnaga (AVEO), Mentha suaveolens (MSEO) and Lavandula stoechas (LSEO), against S. aureus, E. coli and P. aeruginosa, as well as to predict the optimal formulations of these EOs to inhibit bacterial adhesion on the stainless steel (304L SS) surface. EOs were obtained via hydrodistillation and then, their volatile content was analyzed using Gas Chromatography-Mass Spectrometry (GC–MS). The antibacterial activity was evaluated using disc-diffusion, microdilution assays. Moreover, the anti-adhesive effect was assessed using two complementary methods, including contact angle measurements and scanning electron microscopy (SEM) analysis. The GC-MS analysis revealed the presence of various bioactive compounds in AVEO, MSEO and LSEO. The main identified components were piperitenone oxide (52.04%) and pulegone (19.78%) for MSEO; and camphor (23.97 %) and menthol (21.5%) for LSEO. Whereas, AVEO was mainly characterized by the presence of linalool (30.24 %). The antibacterial assays showed that all EOs are able to inhibit the bacterial growth at low concentration (MIC ranged from 0.015 to 4 v/v (%)). The optimization of mixtures antibacterial effect has demonstrated the synergistic effect between the three EOs. The optimal mixture predicted against E. coli, P. aeruginosa, and S. aureus corresponded to 30%/42%/28%, 50%/50%/0%, 43%/30%/27%, and AVEO, LSEO and MSEO, respectively. The contact angle assessment indicated that LSEO, MSEO, AVEO and their optimal mixtures remarkably inhibit bacterial adhesion by the alteration of the physico-chemical properties of 304L SS surface. These results are confirmed by SEM analysis, and proved that EOs formulation was able to inhibit more than 80% of bacterial adhesion to 304L SS surface. In light of these findings, EOs mixtures recommended here, can be applied as a promoting and eco-friendly agent for combating the formation of biofilms on stainless steel surface. |
| first_indexed | 2024-03-09T09:16:12Z |
| format | Article |
| id | doaj.art-d6ddd4e060d1484b9112e0d8507d30a3 |
| institution | Directory Open Access Journal |
| issn | 2468-2276 |
| language | English |
| last_indexed | 2024-03-09T09:16:12Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Scientific African |
| spelling | doaj.art-d6ddd4e060d1484b9112e0d8507d30a32023-12-02T07:06:21ZengElsevierScientific African2468-22762023-11-0122e01927Exploration of Novel Antibacterial and Anti-adhesive Formulations from three Chemically Characterized Essential oils: Optimization using experimental design methodologyNaoufal El Hachlafi0Nesrine Benkhaira1Nouhaila Zouine2Mouhcine Fadil3Mohamed Jeddi4Samir Jeddi5Rachid Flouchi6Saad Ibnsouda Koraichi7Kawtar Fikri-Benbrahim8Laboratory of Microbial Biotechnology and Bioactive Molecules, Sciences and Technologies Faculty, Sidi Mohamed Ben Abdellah University, P. O. Box 2202, Imouzzer Road Fez, Morocco; Corresponding authors.Laboratory of Microbial Biotechnology and Bioactive Molecules, Sciences and Technologies Faculty, Sidi Mohamed Ben Abdellah University, P. O. Box 2202, Imouzzer Road Fez, MoroccoLaboratory of Microbial Biotechnology and Bioactive Molecules, Sciences and Technologies Faculty, Sidi Mohamed Ben Abdellah University, P. O. Box 2202, Imouzzer Road Fez, MoroccoLaboratory of Applied Organic Chemistry, Sidi Mohamed Ben Abdellah University, P.O. Box 2202, Road of Imouzzer, Fez, MoroccoLaboratory of Microbial Biotechnology and Bioactive Molecules, Sciences and Technologies Faculty, Sidi Mohamed Ben Abdellah University, P. O. Box 2202, Imouzzer Road Fez, Morocco; Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health and Quality of Life (SNAMOPEQ), Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez 30 000, MoroccoLaboratory of Microbial Biotechnology and Bioactive Molecules, Sciences and Technologies Faculty, Sidi Mohamed Ben Abdellah University, P. O. Box 2202, Imouzzer Road Fez, MoroccoLaboratory of Microbial Biotechnology and Bioactive Molecules, Sciences and Technologies Faculty, Sidi Mohamed Ben Abdellah University, P. O. Box 2202, Imouzzer Road Fez, Morocco; High Institute of Nursing Professions and Health Technique, Annex Taza, Fez, MoroccoLaboratory of Microbial Biotechnology and Bioactive Molecules, Sciences and Technologies Faculty, Sidi Mohamed Ben Abdellah University, P. O. Box 2202, Imouzzer Road Fez, MoroccoLaboratory of Microbial Biotechnology and Bioactive Molecules, Sciences and Technologies Faculty, Sidi Mohamed Ben Abdellah University, P. O. Box 2202, Imouzzer Road Fez, Morocco; Corresponding authors.In this study, experimental design approach coupled with MIC assay were used to determine the combined treatment effect of three Moroccan essential oils (EOs) derived from Ammi visnaga (AVEO), Mentha suaveolens (MSEO) and Lavandula stoechas (LSEO), against S. aureus, E. coli and P. aeruginosa, as well as to predict the optimal formulations of these EOs to inhibit bacterial adhesion on the stainless steel (304L SS) surface. EOs were obtained via hydrodistillation and then, their volatile content was analyzed using Gas Chromatography-Mass Spectrometry (GC–MS). The antibacterial activity was evaluated using disc-diffusion, microdilution assays. Moreover, the anti-adhesive effect was assessed using two complementary methods, including contact angle measurements and scanning electron microscopy (SEM) analysis. The GC-MS analysis revealed the presence of various bioactive compounds in AVEO, MSEO and LSEO. The main identified components were piperitenone oxide (52.04%) and pulegone (19.78%) for MSEO; and camphor (23.97 %) and menthol (21.5%) for LSEO. Whereas, AVEO was mainly characterized by the presence of linalool (30.24 %). The antibacterial assays showed that all EOs are able to inhibit the bacterial growth at low concentration (MIC ranged from 0.015 to 4 v/v (%)). The optimization of mixtures antibacterial effect has demonstrated the synergistic effect between the three EOs. The optimal mixture predicted against E. coli, P. aeruginosa, and S. aureus corresponded to 30%/42%/28%, 50%/50%/0%, 43%/30%/27%, and AVEO, LSEO and MSEO, respectively. The contact angle assessment indicated that LSEO, MSEO, AVEO and their optimal mixtures remarkably inhibit bacterial adhesion by the alteration of the physico-chemical properties of 304L SS surface. These results are confirmed by SEM analysis, and proved that EOs formulation was able to inhibit more than 80% of bacterial adhesion to 304L SS surface. In light of these findings, EOs mixtures recommended here, can be applied as a promoting and eco-friendly agent for combating the formation of biofilms on stainless steel surface.http://www.sciencedirect.com/science/article/pii/S2468227623003824Essential oilsMixture designAntibacterial activityAdhesionContact anglestainless steel (304L SS) |
| spellingShingle | Naoufal El Hachlafi Nesrine Benkhaira Nouhaila Zouine Mouhcine Fadil Mohamed Jeddi Samir Jeddi Rachid Flouchi Saad Ibnsouda Koraichi Kawtar Fikri-Benbrahim Exploration of Novel Antibacterial and Anti-adhesive Formulations from three Chemically Characterized Essential oils: Optimization using experimental design methodology Scientific African Essential oils Mixture design Antibacterial activity Adhesion Contact angle stainless steel (304L SS) |
| title | Exploration of Novel Antibacterial and Anti-adhesive Formulations from three Chemically Characterized Essential oils: Optimization using experimental design methodology |
| title_full | Exploration of Novel Antibacterial and Anti-adhesive Formulations from three Chemically Characterized Essential oils: Optimization using experimental design methodology |
| title_fullStr | Exploration of Novel Antibacterial and Anti-adhesive Formulations from three Chemically Characterized Essential oils: Optimization using experimental design methodology |
| title_full_unstemmed | Exploration of Novel Antibacterial and Anti-adhesive Formulations from three Chemically Characterized Essential oils: Optimization using experimental design methodology |
| title_short | Exploration of Novel Antibacterial and Anti-adhesive Formulations from three Chemically Characterized Essential oils: Optimization using experimental design methodology |
| title_sort | exploration of novel antibacterial and anti adhesive formulations from three chemically characterized essential oils optimization using experimental design methodology |
| topic | Essential oils Mixture design Antibacterial activity Adhesion Contact angle stainless steel (304L SS) |
| url | http://www.sciencedirect.com/science/article/pii/S2468227623003824 |
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