<it>Candida albicans </it>virulence and drug-resistance requires the <it>O</it>-acyltransferase Gup1p
<p>Abstract</p> <p>Background</p> <p><it>GUP1 </it>gene was primarily identified in <it>Saccharomyces cerevisiae </it>being connected with glycerol uptake defects in association with osmotic stress response. Soon after, Gup1p was implicated in a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-09-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/10/238 |
| _version_ | 1818701595251048448 |
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| author | Ferreira Célia Silva Sónia Faria-Oliveira Fábio Pinho Eva Henriques Mariana Lucas Cândida |
| author_facet | Ferreira Célia Silva Sónia Faria-Oliveira Fábio Pinho Eva Henriques Mariana Lucas Cândida |
| author_sort | Ferreira Célia |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>GUP1 </it>gene was primarily identified in <it>Saccharomyces cerevisiae </it>being connected with glycerol uptake defects in association with osmotic stress response. Soon after, Gup1p was implicated in a complex and extensive series of phenotypes involving major cellular processes. These include membrane and wall maintenance, lipid composition, bud-site selection, cytoskeleton orientation, vacuole morphology, secretory/endocytic pathway, GPI anchors remodelling, and lipid-ordered domains assembly, which is compatible with their inclusion in the Membrane Bound O-acyl transferases (MBOAT) family. In mammals, it has been described as a negative regulator of the Sonic hedgehog pathway involved in morphogenesis, differentiation, proliferation, among other processes.</p> <p>Results</p> <p>We show that <it>Candida albicans </it>Gup1p strongly interferes with the capacity of cells to develop hyphae, to adhere, to invade, and to form a biofilm, all of which are significant virulence factors. Furthermore, the mutant colonies exhibited an aberrant morphology/differentiation pattern. Identically to <it>S. cerevisiae</it>, Ca<it>gup1Δ </it>null mutant was more resistant to antifungals like fluconazole, ketoconazole, and clotrimazole, and displayed an abnormal even sterol distribution at the plasma membrane.</p> <p>Conclusions</p> <p>This work is the first study in the opportunistic yeast <it>Candida albicans</it>, showing a role for the <it>GUP1 </it>gene in virulence as well as in the mechanisms underlying antifungal resistance. Moreover, its impact is even more significant since these results, taken together with all the knowledge about <it>GUP1 </it>gene (from <it>S. cerevisiae </it>and mammals) give consistence to the possibility that Gup1p may be part of a yeast morphogenic pathway parallel to the mammalian Hedgehog.</p> |
| first_indexed | 2024-12-17T15:23:20Z |
| format | Article |
| id | doaj.art-d6de97553cf34d4d98a0115fb7ac2cda |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-17T15:23:20Z |
| publishDate | 2010-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-d6de97553cf34d4d98a0115fb7ac2cda2022-12-21T21:43:20ZengBMCBMC Microbiology1471-21802010-09-0110123810.1186/1471-2180-10-238<it>Candida albicans </it>virulence and drug-resistance requires the <it>O</it>-acyltransferase Gup1pFerreira CéliaSilva SóniaFaria-Oliveira FábioPinho EvaHenriques MarianaLucas Cândida<p>Abstract</p> <p>Background</p> <p><it>GUP1 </it>gene was primarily identified in <it>Saccharomyces cerevisiae </it>being connected with glycerol uptake defects in association with osmotic stress response. Soon after, Gup1p was implicated in a complex and extensive series of phenotypes involving major cellular processes. These include membrane and wall maintenance, lipid composition, bud-site selection, cytoskeleton orientation, vacuole morphology, secretory/endocytic pathway, GPI anchors remodelling, and lipid-ordered domains assembly, which is compatible with their inclusion in the Membrane Bound O-acyl transferases (MBOAT) family. In mammals, it has been described as a negative regulator of the Sonic hedgehog pathway involved in morphogenesis, differentiation, proliferation, among other processes.</p> <p>Results</p> <p>We show that <it>Candida albicans </it>Gup1p strongly interferes with the capacity of cells to develop hyphae, to adhere, to invade, and to form a biofilm, all of which are significant virulence factors. Furthermore, the mutant colonies exhibited an aberrant morphology/differentiation pattern. Identically to <it>S. cerevisiae</it>, Ca<it>gup1Δ </it>null mutant was more resistant to antifungals like fluconazole, ketoconazole, and clotrimazole, and displayed an abnormal even sterol distribution at the plasma membrane.</p> <p>Conclusions</p> <p>This work is the first study in the opportunistic yeast <it>Candida albicans</it>, showing a role for the <it>GUP1 </it>gene in virulence as well as in the mechanisms underlying antifungal resistance. Moreover, its impact is even more significant since these results, taken together with all the knowledge about <it>GUP1 </it>gene (from <it>S. cerevisiae </it>and mammals) give consistence to the possibility that Gup1p may be part of a yeast morphogenic pathway parallel to the mammalian Hedgehog.</p>http://www.biomedcentral.com/1471-2180/10/238 |
| spellingShingle | Ferreira Célia Silva Sónia Faria-Oliveira Fábio Pinho Eva Henriques Mariana Lucas Cândida <it>Candida albicans </it>virulence and drug-resistance requires the <it>O</it>-acyltransferase Gup1p BMC Microbiology |
| title | <it>Candida albicans </it>virulence and drug-resistance requires the <it>O</it>-acyltransferase Gup1p |
| title_full | <it>Candida albicans </it>virulence and drug-resistance requires the <it>O</it>-acyltransferase Gup1p |
| title_fullStr | <it>Candida albicans </it>virulence and drug-resistance requires the <it>O</it>-acyltransferase Gup1p |
| title_full_unstemmed | <it>Candida albicans </it>virulence and drug-resistance requires the <it>O</it>-acyltransferase Gup1p |
| title_short | <it>Candida albicans </it>virulence and drug-resistance requires the <it>O</it>-acyltransferase Gup1p |
| title_sort | it candida albicans it virulence and drug resistance requires the it o it acyltransferase gup1p |
| url | http://www.biomedcentral.com/1471-2180/10/238 |
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