Comparing Hydraulics Between Two Grapevine Cultivars Reveals Differences in Stomatal Regulation Under Water Stress and Exogenous ABA Applications
Hydraulics of plants that have different strategies of stomatal regulation under water stress are relatively poorly understood. We explore how root and shoot hydraulics, stomatal conductance (gs), leaf and root aquaporin (AQP) expression, and abscisic acid (ABA) concentration in leaf xylem sap ([ABA...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.00705/full |
| _version_ | 1811339923589955584 |
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| author | Silvina Dayer Johannes D. Scharwies Sunita A. Ramesh Wendy Sullivan Franziska C. Doerflinger Vinay Pagay Stephen D. Tyerman Stephen D. Tyerman |
| author_facet | Silvina Dayer Johannes D. Scharwies Sunita A. Ramesh Wendy Sullivan Franziska C. Doerflinger Vinay Pagay Stephen D. Tyerman Stephen D. Tyerman |
| author_sort | Silvina Dayer |
| collection | DOAJ |
| description | Hydraulics of plants that have different strategies of stomatal regulation under water stress are relatively poorly understood. We explore how root and shoot hydraulics, stomatal conductance (gs), leaf and root aquaporin (AQP) expression, and abscisic acid (ABA) concentration in leaf xylem sap ([ABA]xylemsap) may be coordinated under mild water stress and exogenous ABA applications in two Vitis vinifera L. cultivars traditionally classified as near-isohydric (Grenache) and near-anisohydric (Syrah). Under water stress, Grenache exhibited stronger adjustments of plant and root hydraulic conductances and greater stomatal sensitivity to [ABA]xylemsap than Syrah resulting in greater conservation of soil moisture but not necessarily more isohydric behavior. Correlations between leaf (Ψleaf) and predawn (ΨPD) water potentials between cultivars suggested a “hydrodynamic” behavior rather than a particular iso-anisohydric classification. A significant decrease of Ψleaf in well-watered ABA-fed vines supported a role of ABA in the soil-leaf hydraulic pathway to regulate gs. Correlations between leaf and root AQPs expression levels under water deficit could explain the response of leaf (Kleaf) and root (Lpr) hydraulic conductances in both cultivars. Additional studies under a wider range of soil water deficits are required to explore the possible differential regulation of gs and plant hydraulics in different cultivars and experimental conditions. |
| first_indexed | 2024-04-13T18:33:39Z |
| format | Article |
| id | doaj.art-d6e469505569485e818ab3b130a53656 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T18:33:39Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-d6e469505569485e818ab3b130a536562022-12-22T02:34:58ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-06-011110.3389/fpls.2020.00705526571Comparing Hydraulics Between Two Grapevine Cultivars Reveals Differences in Stomatal Regulation Under Water Stress and Exogenous ABA ApplicationsSilvina Dayer0Johannes D. Scharwies1Sunita A. Ramesh2Wendy Sullivan3Franziska C. Doerflinger4Vinay Pagay5Stephen D. Tyerman6Stephen D. Tyerman7School of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, SA, AustraliaSchool of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, SA, AustraliaSchool of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, SA, AustraliaSchool of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, SA, AustraliaSchool of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, SA, AustraliaSchool of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, SA, AustraliaSchool of Agriculture, Food and Wine, The University of Adelaide, Glen Osmond, SA, AustraliaAustralian Research Council Centre of Excellence in Plant Energy Biology, Waite Research Institute, School of Agriculture, Food and Wine, The University of Adelaide, Adelaide, SA, AustraliaHydraulics of plants that have different strategies of stomatal regulation under water stress are relatively poorly understood. We explore how root and shoot hydraulics, stomatal conductance (gs), leaf and root aquaporin (AQP) expression, and abscisic acid (ABA) concentration in leaf xylem sap ([ABA]xylemsap) may be coordinated under mild water stress and exogenous ABA applications in two Vitis vinifera L. cultivars traditionally classified as near-isohydric (Grenache) and near-anisohydric (Syrah). Under water stress, Grenache exhibited stronger adjustments of plant and root hydraulic conductances and greater stomatal sensitivity to [ABA]xylemsap than Syrah resulting in greater conservation of soil moisture but not necessarily more isohydric behavior. Correlations between leaf (Ψleaf) and predawn (ΨPD) water potentials between cultivars suggested a “hydrodynamic” behavior rather than a particular iso-anisohydric classification. A significant decrease of Ψleaf in well-watered ABA-fed vines supported a role of ABA in the soil-leaf hydraulic pathway to regulate gs. Correlations between leaf and root AQPs expression levels under water deficit could explain the response of leaf (Kleaf) and root (Lpr) hydraulic conductances in both cultivars. Additional studies under a wider range of soil water deficits are required to explore the possible differential regulation of gs and plant hydraulics in different cultivars and experimental conditions.https://www.frontiersin.org/article/10.3389/fpls.2020.00705/fullVitis viniferagas exchangeaquaporinplasma membrane intrinsic proteintonoplast intrinsic proteinhydraulic conductivity |
| spellingShingle | Silvina Dayer Johannes D. Scharwies Sunita A. Ramesh Wendy Sullivan Franziska C. Doerflinger Vinay Pagay Stephen D. Tyerman Stephen D. Tyerman Comparing Hydraulics Between Two Grapevine Cultivars Reveals Differences in Stomatal Regulation Under Water Stress and Exogenous ABA Applications Frontiers in Plant Science Vitis vinifera gas exchange aquaporin plasma membrane intrinsic protein tonoplast intrinsic protein hydraulic conductivity |
| title | Comparing Hydraulics Between Two Grapevine Cultivars Reveals Differences in Stomatal Regulation Under Water Stress and Exogenous ABA Applications |
| title_full | Comparing Hydraulics Between Two Grapevine Cultivars Reveals Differences in Stomatal Regulation Under Water Stress and Exogenous ABA Applications |
| title_fullStr | Comparing Hydraulics Between Two Grapevine Cultivars Reveals Differences in Stomatal Regulation Under Water Stress and Exogenous ABA Applications |
| title_full_unstemmed | Comparing Hydraulics Between Two Grapevine Cultivars Reveals Differences in Stomatal Regulation Under Water Stress and Exogenous ABA Applications |
| title_short | Comparing Hydraulics Between Two Grapevine Cultivars Reveals Differences in Stomatal Regulation Under Water Stress and Exogenous ABA Applications |
| title_sort | comparing hydraulics between two grapevine cultivars reveals differences in stomatal regulation under water stress and exogenous aba applications |
| topic | Vitis vinifera gas exchange aquaporin plasma membrane intrinsic protein tonoplast intrinsic protein hydraulic conductivity |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.00705/full |
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