The Effects of Honeysuckle (<i>Lonicera caerulea</i> L.) Berry Iridoid-Anthocyanin Extract on the Intestinal and Muscle Histopathology in Mice during Experimental Trichinellosis
The aim of the study was to determine the effect of iridoid-anthocyanin extract from honeysuckle (<i>Lonicera caerulea</i> L.) (LC) berries on histopathological changes in the intestines and muscles during experimental trichinellosis in mice. The LC extract was administered to uninfected...
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MDPI AG
2023-10-01
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| Online Access: | https://www.mdpi.com/1420-3049/28/20/7067 |
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| author | Jolanta Piekarska Jan P. Madej Michał Gorczykowski Marianna Szczypka |
| author_facet | Jolanta Piekarska Jan P. Madej Michał Gorczykowski Marianna Szczypka |
| author_sort | Jolanta Piekarska |
| collection | DOAJ |
| description | The aim of the study was to determine the effect of iridoid-anthocyanin extract from honeysuckle (<i>Lonicera caerulea</i> L.) (LC) berries on histopathological changes in the intestines and muscles during experimental trichinellosis in mice. The LC extract was administered to uninfected mice (LC group) and <i>Trichinella-spiralis</i>-infected mice (T+LC) orally at a dose of 2 g/kg bw, six times at 24 h intervals, from day 3 prior to infection to day 3 post-infection (dpi). Jejunum samples were collected on 5, 7, 14, and 21 dpi, and their histological assessment involved the villus height to crypt depth ratio (VH/CD), goblet cell (GC) number, and morphological changes. In the <i>T. spiralis</i>-infected muscles, the extent of inflammatory infiltration on the 14th and 21st dpi was assessed. LC in the infected mice restored the VH/CD ratio to control values on 14 dpi. A beneficial effect of the LC extract on the villus height was also observed 14 dpi in the LC and T+LC groups. No differences in the extent of inflammatory infiltration in the muscles between the T+LC and T groups were observed. In conclusion, the iridoid-anthocyanin extract from honeysuckle berry contributed to alleviating the symptoms of the intestinal phase of <i>T. spiralis</i> infection. |
| first_indexed | 2024-03-10T21:01:03Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T21:01:03Z |
| publishDate | 2023-10-01 |
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| series | Molecules |
| spelling | doaj.art-d6e53c1f666940498602b3568c10b7272023-11-19T17:32:16ZengMDPI AGMolecules1420-30492023-10-012820706710.3390/molecules28207067The Effects of Honeysuckle (<i>Lonicera caerulea</i> L.) Berry Iridoid-Anthocyanin Extract on the Intestinal and Muscle Histopathology in Mice during Experimental TrichinellosisJolanta Piekarska0Jan P. Madej1Michał Gorczykowski2Marianna Szczypka3Division of Parasitology, Department of Internal Medicine and Clinic of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroclaw, PolandDepartment of Immunology, Pathophysiology and Veterinary Preventive Medicine, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroclaw, PolandDivision of Parasitology, Department of Internal Medicine and Clinic of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroclaw, PolandDepartment of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Norwida 31, 50-375 Wroclaw, PolandThe aim of the study was to determine the effect of iridoid-anthocyanin extract from honeysuckle (<i>Lonicera caerulea</i> L.) (LC) berries on histopathological changes in the intestines and muscles during experimental trichinellosis in mice. The LC extract was administered to uninfected mice (LC group) and <i>Trichinella-spiralis</i>-infected mice (T+LC) orally at a dose of 2 g/kg bw, six times at 24 h intervals, from day 3 prior to infection to day 3 post-infection (dpi). Jejunum samples were collected on 5, 7, 14, and 21 dpi, and their histological assessment involved the villus height to crypt depth ratio (VH/CD), goblet cell (GC) number, and morphological changes. In the <i>T. spiralis</i>-infected muscles, the extent of inflammatory infiltration on the 14th and 21st dpi was assessed. LC in the infected mice restored the VH/CD ratio to control values on 14 dpi. A beneficial effect of the LC extract on the villus height was also observed 14 dpi in the LC and T+LC groups. No differences in the extent of inflammatory infiltration in the muscles between the T+LC and T groups were observed. In conclusion, the iridoid-anthocyanin extract from honeysuckle berry contributed to alleviating the symptoms of the intestinal phase of <i>T. spiralis</i> infection.https://www.mdpi.com/1420-3049/28/20/7067<i>Lonicera caerulea</i> L.<i>T. spiralis</i>intestinemusclemorphology |
| spellingShingle | Jolanta Piekarska Jan P. Madej Michał Gorczykowski Marianna Szczypka The Effects of Honeysuckle (<i>Lonicera caerulea</i> L.) Berry Iridoid-Anthocyanin Extract on the Intestinal and Muscle Histopathology in Mice during Experimental Trichinellosis Molecules <i>Lonicera caerulea</i> L. <i>T. spiralis</i> intestine muscle morphology |
| title | The Effects of Honeysuckle (<i>Lonicera caerulea</i> L.) Berry Iridoid-Anthocyanin Extract on the Intestinal and Muscle Histopathology in Mice during Experimental Trichinellosis |
| title_full | The Effects of Honeysuckle (<i>Lonicera caerulea</i> L.) Berry Iridoid-Anthocyanin Extract on the Intestinal and Muscle Histopathology in Mice during Experimental Trichinellosis |
| title_fullStr | The Effects of Honeysuckle (<i>Lonicera caerulea</i> L.) Berry Iridoid-Anthocyanin Extract on the Intestinal and Muscle Histopathology in Mice during Experimental Trichinellosis |
| title_full_unstemmed | The Effects of Honeysuckle (<i>Lonicera caerulea</i> L.) Berry Iridoid-Anthocyanin Extract on the Intestinal and Muscle Histopathology in Mice during Experimental Trichinellosis |
| title_short | The Effects of Honeysuckle (<i>Lonicera caerulea</i> L.) Berry Iridoid-Anthocyanin Extract on the Intestinal and Muscle Histopathology in Mice during Experimental Trichinellosis |
| title_sort | effects of honeysuckle i lonicera caerulea i l berry iridoid anthocyanin extract on the intestinal and muscle histopathology in mice during experimental trichinellosis |
| topic | <i>Lonicera caerulea</i> L. <i>T. spiralis</i> intestine muscle morphology |
| url | https://www.mdpi.com/1420-3049/28/20/7067 |
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