Protective effect of Tualang honey against cadmium-induced morphological abnormalities and oxidative stress in the ovary of rats
Abstract Background To investigate the protective effects of Tualang honey against the toxicity effects induced by cadmium (Cd) on the ovary. Methods A total of 32 female Sprague Dawley rats were taken and randomly divided into four groups (n = 8). Throughout the experimental period of 6 weeks, nega...
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BMC
2020-05-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-020-02960-1 |
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| author | Siti Suraya Ruslee Siti Sarah Mohamad Zaid Ikmal Hisyam Bakrin Yong Meng Goh Noordin Mohamed Mustapha |
| author_facet | Siti Suraya Ruslee Siti Sarah Mohamad Zaid Ikmal Hisyam Bakrin Yong Meng Goh Noordin Mohamed Mustapha |
| author_sort | Siti Suraya Ruslee |
| collection | DOAJ |
| description | Abstract Background To investigate the protective effects of Tualang honey against the toxicity effects induced by cadmium (Cd) on the ovary. Methods A total of 32 female Sprague Dawley rats were taken and randomly divided into four groups (n = 8). Throughout the experimental period of 6 weeks, negative control-NC (vehicle deionized water), positive control-CD (Cd at 5 mg/kg), Tualang honey followed by Cd exposure-TH (Tualang honey at 200 mg/kg and Cd at 5 mg/kg) and Tualang honey control-THC (Tualang honey at 200 mg/kg) groups, were administered orally on a daily basis. Results Rats exposed to Cd were significantly higher in ovarian weight, number of antral and atretic follicles as compared to the NC group. The disruptive effects of Cd on ovarian follicles were associated with a disruption in gonadotropin hormones and decreases in follicular stimulating hormone (FSH) and luteinizing hormone (LH). Moreover, a significant formation of oxidative stress in ovarian Cd-exposed rats has been proven by increasing the level of lipid peroxidation products (malondialdehyde) and decreasing the levels of enzymatic antioxidant (catalase). Interestingly, a daily supplementation of high antioxidant agents such as Tualang honey in these animals, caused significant improvements in the histological changes. Additionally, less atretic follicles were observed, restoring the normal level of LH and FSH (P < 0.001), and normalizing the ovarian malondialdehyde (P < 0.05) and catalase levels in comparison with CD group (P < 0.05). Conclusions Tualang honey has protective effects against Cd-induced ovarian toxicity by reducing morphological abnormalities, restoring the normal levels of gonadotropin hormones and stabilizing equilibrium levels of lipid peroxidation and antioxidant enzyme in ovaries of rats. |
| first_indexed | 2024-12-13T11:53:17Z |
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| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-12-13T11:53:17Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-d6e633752bd4434d9a9c5e5c77289fde2022-12-21T23:47:17ZengBMCBMC Complementary Medicine and Therapies2662-76712020-05-0120111110.1186/s12906-020-02960-1Protective effect of Tualang honey against cadmium-induced morphological abnormalities and oxidative stress in the ovary of ratsSiti Suraya Ruslee0Siti Sarah Mohamad Zaid1Ikmal Hisyam Bakrin2Yong Meng Goh3Noordin Mohamed Mustapha4Department of Environment, Faculty of Forestry and Environment, Universiti Putra MalaysiaDepartment of Environment, Faculty of Forestry and Environment, Universiti Putra MalaysiaDepartment of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra MalaysiaDepartment of Veterinary Preclinical Science, Faculty of Veterinary Medicine, Universiti Putra MalaysiaDepartment of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra MalaysiaAbstract Background To investigate the protective effects of Tualang honey against the toxicity effects induced by cadmium (Cd) on the ovary. Methods A total of 32 female Sprague Dawley rats were taken and randomly divided into four groups (n = 8). Throughout the experimental period of 6 weeks, negative control-NC (vehicle deionized water), positive control-CD (Cd at 5 mg/kg), Tualang honey followed by Cd exposure-TH (Tualang honey at 200 mg/kg and Cd at 5 mg/kg) and Tualang honey control-THC (Tualang honey at 200 mg/kg) groups, were administered orally on a daily basis. Results Rats exposed to Cd were significantly higher in ovarian weight, number of antral and atretic follicles as compared to the NC group. The disruptive effects of Cd on ovarian follicles were associated with a disruption in gonadotropin hormones and decreases in follicular stimulating hormone (FSH) and luteinizing hormone (LH). Moreover, a significant formation of oxidative stress in ovarian Cd-exposed rats has been proven by increasing the level of lipid peroxidation products (malondialdehyde) and decreasing the levels of enzymatic antioxidant (catalase). Interestingly, a daily supplementation of high antioxidant agents such as Tualang honey in these animals, caused significant improvements in the histological changes. Additionally, less atretic follicles were observed, restoring the normal level of LH and FSH (P < 0.001), and normalizing the ovarian malondialdehyde (P < 0.05) and catalase levels in comparison with CD group (P < 0.05). Conclusions Tualang honey has protective effects against Cd-induced ovarian toxicity by reducing morphological abnormalities, restoring the normal levels of gonadotropin hormones and stabilizing equilibrium levels of lipid peroxidation and antioxidant enzyme in ovaries of rats.http://link.springer.com/article/10.1186/s12906-020-02960-1Tualang honeyCadmiumOvaryAntioxidantOxidative stress |
| spellingShingle | Siti Suraya Ruslee Siti Sarah Mohamad Zaid Ikmal Hisyam Bakrin Yong Meng Goh Noordin Mohamed Mustapha Protective effect of Tualang honey against cadmium-induced morphological abnormalities and oxidative stress in the ovary of rats BMC Complementary Medicine and Therapies Tualang honey Cadmium Ovary Antioxidant Oxidative stress |
| title | Protective effect of Tualang honey against cadmium-induced morphological abnormalities and oxidative stress in the ovary of rats |
| title_full | Protective effect of Tualang honey against cadmium-induced morphological abnormalities and oxidative stress in the ovary of rats |
| title_fullStr | Protective effect of Tualang honey against cadmium-induced morphological abnormalities and oxidative stress in the ovary of rats |
| title_full_unstemmed | Protective effect of Tualang honey against cadmium-induced morphological abnormalities and oxidative stress in the ovary of rats |
| title_short | Protective effect of Tualang honey against cadmium-induced morphological abnormalities and oxidative stress in the ovary of rats |
| title_sort | protective effect of tualang honey against cadmium induced morphological abnormalities and oxidative stress in the ovary of rats |
| topic | Tualang honey Cadmium Ovary Antioxidant Oxidative stress |
| url | http://link.springer.com/article/10.1186/s12906-020-02960-1 |
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