Somatic embryogenesis from stem segment of Hyoscyamus arachnoideus Pojark. and qualitative and quantitative evaluation of proteins during different stages of it
The Hyoscyamus genus has been always regarded because of the therapeutic effects and its contained tropan alkaloids. Utilizing genetic engineering methods for production of plants for production of plant belonging to this genus with higher medical values and propagation of this plant in a fast and c...
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| Format: | Article |
| Language: | fas |
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Islamic Azad University - Gorgan Branch
2007-02-01
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| Series: | فیزیولوژی محیطی گیاهی |
| Subjects: | |
| Online Access: | https://ecophysiologi.gorgan.iau.ir/article_692788_35c5bdd8b61b471af5bd72f1b2dd0d47.pdf |
| _version_ | 1797808295993409536 |
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| author | mahdis Ebrahimzadeh mehlgha Ghorbanli hasan Ebrahimzadeh |
| author_facet | mahdis Ebrahimzadeh mehlgha Ghorbanli hasan Ebrahimzadeh |
| author_sort | mahdis Ebrahimzadeh |
| collection | DOAJ |
| description | The Hyoscyamus genus has been always regarded because of the therapeutic effects and its contained tropan alkaloids. Utilizing genetic engineering methods for production of plants for production of plant belonging to this genus with higher medical values and propagation of this plant in a fast and cost-effective manner is very important. Somatic embryogenesis is a great help in this field. The aim of the current study is to gain knowledge about mechanism of somatic embryogenesis via protein changes' assessment in different stages of embryo formation. In this study stem culture on MS medium containing BAP and IAA lead to embryogenesis.
Quantitative protein evaluation in different stages of embryo development showed changes in protein content. The qualitative evaluation of protein was performed using electrophoresis of two types of polyacryl amide gel in discontinued system (PAGE and SDS-PAGE). Molecular mass of peptide as well as peptide separated bonds were calculated, and it cleared that during embryogenesis different proteins and peptides undergo up-regulation, down-regulation and modulation. In addition, main and fundamental proteins and peptides were known. |
| first_indexed | 2024-03-13T06:35:28Z |
| format | Article |
| id | doaj.art-d6e63aed283d4b4ba0e88d39a2ff46ec |
| institution | Directory Open Access Journal |
| issn | 2423-7671 2783-4689 |
| language | fas |
| last_indexed | 2024-03-13T06:35:28Z |
| publishDate | 2007-02-01 |
| publisher | Islamic Azad University - Gorgan Branch |
| record_format | Article |
| series | فیزیولوژی محیطی گیاهی |
| spelling | doaj.art-d6e63aed283d4b4ba0e88d39a2ff46ec2023-06-09T06:23:31ZfasIslamic Azad University - Gorgan Branchفیزیولوژی محیطی گیاهی2423-76712783-46892007-02-0114113692788Somatic embryogenesis from stem segment of Hyoscyamus arachnoideus Pojark. and qualitative and quantitative evaluation of proteins during different stages of itmahdis Ebrahimzadeh0mehlgha Ghorbanli1hasan Ebrahimzadeh2Department of biology, Faculty of Science Islamic, Azad University Branch, Science and ResearchDepartment of biology, Faculty of Science Islamic, Azad University Branch, GorganDepartment of biology, Faculty of Science Islamic, Tehran University, TehranThe Hyoscyamus genus has been always regarded because of the therapeutic effects and its contained tropan alkaloids. Utilizing genetic engineering methods for production of plants for production of plant belonging to this genus with higher medical values and propagation of this plant in a fast and cost-effective manner is very important. Somatic embryogenesis is a great help in this field. The aim of the current study is to gain knowledge about mechanism of somatic embryogenesis via protein changes' assessment in different stages of embryo formation. In this study stem culture on MS medium containing BAP and IAA lead to embryogenesis. Quantitative protein evaluation in different stages of embryo development showed changes in protein content. The qualitative evaluation of protein was performed using electrophoresis of two types of polyacryl amide gel in discontinued system (PAGE and SDS-PAGE). Molecular mass of peptide as well as peptide separated bonds were calculated, and it cleared that during embryogenesis different proteins and peptides undergo up-regulation, down-regulation and modulation. In addition, main and fundamental proteins and peptides were known.https://ecophysiologi.gorgan.iau.ir/article_692788_35c5bdd8b61b471af5bd72f1b2dd0d47.pdfelectrophoresisembryogenesishyoscyamus arachnoideusprotein |
| spellingShingle | mahdis Ebrahimzadeh mehlgha Ghorbanli hasan Ebrahimzadeh Somatic embryogenesis from stem segment of Hyoscyamus arachnoideus Pojark. and qualitative and quantitative evaluation of proteins during different stages of it فیزیولوژی محیطی گیاهی electrophoresis embryogenesis hyoscyamus arachnoideus protein |
| title | Somatic embryogenesis from stem segment of Hyoscyamus arachnoideus Pojark. and qualitative and quantitative evaluation of proteins during different stages of it |
| title_full | Somatic embryogenesis from stem segment of Hyoscyamus arachnoideus Pojark. and qualitative and quantitative evaluation of proteins during different stages of it |
| title_fullStr | Somatic embryogenesis from stem segment of Hyoscyamus arachnoideus Pojark. and qualitative and quantitative evaluation of proteins during different stages of it |
| title_full_unstemmed | Somatic embryogenesis from stem segment of Hyoscyamus arachnoideus Pojark. and qualitative and quantitative evaluation of proteins during different stages of it |
| title_short | Somatic embryogenesis from stem segment of Hyoscyamus arachnoideus Pojark. and qualitative and quantitative evaluation of proteins during different stages of it |
| title_sort | somatic embryogenesis from stem segment of hyoscyamus arachnoideus pojark and qualitative and quantitative evaluation of proteins during different stages of it |
| topic | electrophoresis embryogenesis hyoscyamus arachnoideus protein |
| url | https://ecophysiologi.gorgan.iau.ir/article_692788_35c5bdd8b61b471af5bd72f1b2dd0d47.pdf |
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