Performance of six diagnostic tests to screen for Chagas disease in blood banks and prevalence of Trypanosoma cruzi infection among donors with inconclusive serology screening based on the analysis of epidemiological variables
OBJECTIVE: The frequent occurrence of inconclusive serology in blood banks and the absence of a gold standard test for Chagas'disease led us to examine the efficacy of the blood culture test and five commercial tests (ELISA, IIF, HAI, c-ELISA, rec-ELISA) used in screening blood donors for Chaga...
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| Format: | Article |
| Language: | English |
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Elsevier
2012-01-01
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| Series: | Revista Brasileira de Hematologia e Hemoterapia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842012000400014 |
| _version_ | 1818021551584313344 |
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| author | Gilberto de Araujo Pereira Francisco Louzada-Neto Valdirene de Fátima Barbosa Márcia Maria Ferreira-Silva Helio de Moraes-Souza |
| author_facet | Gilberto de Araujo Pereira Francisco Louzada-Neto Valdirene de Fátima Barbosa Márcia Maria Ferreira-Silva Helio de Moraes-Souza |
| author_sort | Gilberto de Araujo Pereira |
| collection | DOAJ |
| description | OBJECTIVE: The frequent occurrence of inconclusive serology in blood banks and the absence of a gold standard test for Chagas'disease led us to examine the efficacy of the blood culture test and five commercial tests (ELISA, IIF, HAI, c-ELISA, rec-ELISA) used in screening blood donors for Chagas disease, as well as to investigate the prevalence of Trypanosoma cruzi infection among donors with inconclusive serology screening in respect to some epidemiological variables. METHODS: To obtain estimates of interest we considered a Bayesian latent class model with inclusion of covariates from the logit link. RESULTS: A better performance was observed with some categories of epidemiological variables. In addition, all pairs of tests (excluding the blood culture test) presented as good alternatives for both screening (sensitivity > 99.96% in parallel testing) and for confirmation (specificity > 99.93% in serial testing) of Chagas disease. The prevalence of 13.30% observed in the stratum of donors with inconclusive serology, means that probably most of these are non-reactive serology. In addition, depending on the level of specific epidemiological variables, the absence of infection can be predicted with a probability of 100% in this group from the pairs of tests using parallel testing. CONCLUSION: The epidemiological variables can lead to improved test results and thus assist in the clarification of inconclusive serology screening results. Moreover, all combinations of pairs using the five commercial tests are good alternatives to confirm results. |
| first_indexed | 2024-04-14T08:19:37Z |
| format | Article |
| id | doaj.art-d6e82310f6824996b11c4308565e1a63 |
| institution | Directory Open Access Journal |
| issn | 1516-8484 1806-0870 |
| language | English |
| last_indexed | 2024-04-14T08:19:37Z |
| publishDate | 2012-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Revista Brasileira de Hematologia e Hemoterapia |
| spelling | doaj.art-d6e82310f6824996b11c4308565e1a632022-12-22T02:04:16ZengElsevierRevista Brasileira de Hematologia e Hemoterapia1516-84841806-08702012-01-0134429229710.5581/1516-8484.20120074Performance of six diagnostic tests to screen for Chagas disease in blood banks and prevalence of Trypanosoma cruzi infection among donors with inconclusive serology screening based on the analysis of epidemiological variablesGilberto de Araujo PereiraFrancisco Louzada-NetoValdirene de Fátima BarbosaMárcia Maria Ferreira-SilvaHelio de Moraes-SouzaOBJECTIVE: The frequent occurrence of inconclusive serology in blood banks and the absence of a gold standard test for Chagas'disease led us to examine the efficacy of the blood culture test and five commercial tests (ELISA, IIF, HAI, c-ELISA, rec-ELISA) used in screening blood donors for Chagas disease, as well as to investigate the prevalence of Trypanosoma cruzi infection among donors with inconclusive serology screening in respect to some epidemiological variables. METHODS: To obtain estimates of interest we considered a Bayesian latent class model with inclusion of covariates from the logit link. RESULTS: A better performance was observed with some categories of epidemiological variables. In addition, all pairs of tests (excluding the blood culture test) presented as good alternatives for both screening (sensitivity > 99.96% in parallel testing) and for confirmation (specificity > 99.93% in serial testing) of Chagas disease. The prevalence of 13.30% observed in the stratum of donors with inconclusive serology, means that probably most of these are non-reactive serology. In addition, depending on the level of specific epidemiological variables, the absence of infection can be predicted with a probability of 100% in this group from the pairs of tests using parallel testing. CONCLUSION: The epidemiological variables can lead to improved test results and thus assist in the clarification of inconclusive serology screening results. Moreover, all combinations of pairs using the five commercial tests are good alternatives to confirm results.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842012000400014Blood donorsChagas diseaseSensitivity and specificityEpidemiologic factors |
| spellingShingle | Gilberto de Araujo Pereira Francisco Louzada-Neto Valdirene de Fátima Barbosa Márcia Maria Ferreira-Silva Helio de Moraes-Souza Performance of six diagnostic tests to screen for Chagas disease in blood banks and prevalence of Trypanosoma cruzi infection among donors with inconclusive serology screening based on the analysis of epidemiological variables Revista Brasileira de Hematologia e Hemoterapia Blood donors Chagas disease Sensitivity and specificity Epidemiologic factors |
| title | Performance of six diagnostic tests to screen for Chagas disease in blood banks and prevalence of Trypanosoma cruzi infection among donors with inconclusive serology screening based on the analysis of epidemiological variables |
| title_full | Performance of six diagnostic tests to screen for Chagas disease in blood banks and prevalence of Trypanosoma cruzi infection among donors with inconclusive serology screening based on the analysis of epidemiological variables |
| title_fullStr | Performance of six diagnostic tests to screen for Chagas disease in blood banks and prevalence of Trypanosoma cruzi infection among donors with inconclusive serology screening based on the analysis of epidemiological variables |
| title_full_unstemmed | Performance of six diagnostic tests to screen for Chagas disease in blood banks and prevalence of Trypanosoma cruzi infection among donors with inconclusive serology screening based on the analysis of epidemiological variables |
| title_short | Performance of six diagnostic tests to screen for Chagas disease in blood banks and prevalence of Trypanosoma cruzi infection among donors with inconclusive serology screening based on the analysis of epidemiological variables |
| title_sort | performance of six diagnostic tests to screen for chagas disease in blood banks and prevalence of trypanosoma cruzi infection among donors with inconclusive serology screening based on the analysis of epidemiological variables |
| topic | Blood donors Chagas disease Sensitivity and specificity Epidemiologic factors |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842012000400014 |
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