Anti-Proliferative Activity of Poloxamer Cobalt Ferrite Nanoparticles against Human Prostate Cancer (DU-145) Cells: In-Vitro Study
Prostate cancer is the second most frequent type of cancer death in men. This study refers to the novel hyperthermia application of poloxamer-coated cobalt ferrite as a new approach for thermal eradication of DU-145 human prostate cancerous cells under a radio frequency magnetic field (RF-MF). The h...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi-IET
2024-01-01
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| Series: | IET Nanobiotechnology |
| Online Access: | http://dx.doi.org/10.1049/2024/8929168 |
| _version_ | 1797238500266868736 |
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| author | Nazanin Oroskhani Seyed Mohammad Amini Sakine Shirvalilou Mehdi Khodaie Seyed Rabi Mahdavi |
| author_facet | Nazanin Oroskhani Seyed Mohammad Amini Sakine Shirvalilou Mehdi Khodaie Seyed Rabi Mahdavi |
| author_sort | Nazanin Oroskhani |
| collection | DOAJ |
| description | Prostate cancer is the second most frequent type of cancer death in men. This study refers to the novel hyperthermia application of poloxamer-coated cobalt ferrite as a new approach for thermal eradication of DU-145 human prostate cancerous cells under a radio frequency magnetic field (RF-MF). The hydrothermal method was applied for the synthesis of cobalt ferrite nanoparticles. Then, the structure, size, and morphology of nanoparticle were characterized. The cytotoxicity of the synthesized nanoparticles and RF-MF exposure on DU-145 prostate cancer cells was investigated separately or in combination with colony formation methods and MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] assay. Transmission electron microscopy (TEM) confirmed the spherical morphology of nanoparticles with a size of 5.5 ± 2.6 nm. The temperature of cells treated with nanoparticles under RF-MF reached 42.73 ± 0.2°C after 15 min. RF-MF treatment or nanoparticles have not affected cell viability significantly. However, the combination of them eradicated 53% ± 4% of cancerous cells. In-vitro hyperthermia was performed on human prostate cancer cells (DU-145) with cobalt ferrite nanoparticles at specific concentrations that demonstrated a decrease in survival fraction based on colony formation assay compared to cells that were treated alone with nanoparticles or with RF-MF. |
| first_indexed | 2024-04-24T17:36:38Z |
| format | Article |
| id | doaj.art-d6eb7e7c968849c7ad5c9f81c57d1a8f |
| institution | Directory Open Access Journal |
| issn | 1751-875X |
| language | English |
| last_indexed | 2024-04-24T17:36:38Z |
| publishDate | 2024-01-01 |
| publisher | Hindawi-IET |
| record_format | Article |
| series | IET Nanobiotechnology |
| spelling | doaj.art-d6eb7e7c968849c7ad5c9f81c57d1a8f2024-03-28T05:00:32ZengHindawi-IETIET Nanobiotechnology1751-875X2024-01-01202410.1049/2024/8929168Anti-Proliferative Activity of Poloxamer Cobalt Ferrite Nanoparticles against Human Prostate Cancer (DU-145) Cells: In-Vitro StudyNazanin Oroskhani0Seyed Mohammad Amini1Sakine Shirvalilou2Mehdi Khodaie3Seyed Rabi Mahdavi4Department of Medical PhysicsRadiation Biology Research CenterFinetech in Medicine Research CenterMaterials Science and EngineeringDepartment of Medical PhysicsProstate cancer is the second most frequent type of cancer death in men. This study refers to the novel hyperthermia application of poloxamer-coated cobalt ferrite as a new approach for thermal eradication of DU-145 human prostate cancerous cells under a radio frequency magnetic field (RF-MF). The hydrothermal method was applied for the synthesis of cobalt ferrite nanoparticles. Then, the structure, size, and morphology of nanoparticle were characterized. The cytotoxicity of the synthesized nanoparticles and RF-MF exposure on DU-145 prostate cancer cells was investigated separately or in combination with colony formation methods and MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] assay. Transmission electron microscopy (TEM) confirmed the spherical morphology of nanoparticles with a size of 5.5 ± 2.6 nm. The temperature of cells treated with nanoparticles under RF-MF reached 42.73 ± 0.2°C after 15 min. RF-MF treatment or nanoparticles have not affected cell viability significantly. However, the combination of them eradicated 53% ± 4% of cancerous cells. In-vitro hyperthermia was performed on human prostate cancer cells (DU-145) with cobalt ferrite nanoparticles at specific concentrations that demonstrated a decrease in survival fraction based on colony formation assay compared to cells that were treated alone with nanoparticles or with RF-MF.http://dx.doi.org/10.1049/2024/8929168 |
| spellingShingle | Nazanin Oroskhani Seyed Mohammad Amini Sakine Shirvalilou Mehdi Khodaie Seyed Rabi Mahdavi Anti-Proliferative Activity of Poloxamer Cobalt Ferrite Nanoparticles against Human Prostate Cancer (DU-145) Cells: In-Vitro Study IET Nanobiotechnology |
| title | Anti-Proliferative Activity of Poloxamer Cobalt Ferrite Nanoparticles against Human Prostate Cancer (DU-145) Cells: In-Vitro Study |
| title_full | Anti-Proliferative Activity of Poloxamer Cobalt Ferrite Nanoparticles against Human Prostate Cancer (DU-145) Cells: In-Vitro Study |
| title_fullStr | Anti-Proliferative Activity of Poloxamer Cobalt Ferrite Nanoparticles against Human Prostate Cancer (DU-145) Cells: In-Vitro Study |
| title_full_unstemmed | Anti-Proliferative Activity of Poloxamer Cobalt Ferrite Nanoparticles against Human Prostate Cancer (DU-145) Cells: In-Vitro Study |
| title_short | Anti-Proliferative Activity of Poloxamer Cobalt Ferrite Nanoparticles against Human Prostate Cancer (DU-145) Cells: In-Vitro Study |
| title_sort | anti proliferative activity of poloxamer cobalt ferrite nanoparticles against human prostate cancer du 145 cells in vitro study |
| url | http://dx.doi.org/10.1049/2024/8929168 |
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