Verification and Analysis of Sheep Tail Type-Associated <i>PDGF-D</i> Gene Polymorphisms
The aim of this study was to examine the correlation between the platelet-derived growth factor-D (<i>PDGF-D</i>) gene and sheep tail type character and explore the potential underlying mechanism. A total of 533 sheep were included in this study. Polymorphic sites were examined by Pool-s...
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MDPI AG
2020-01-01
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| author | Qing Li Zengkui Lu Meilin Jin Xiaojuan Fei Kai Quan Yongbin Liu Lin Ma Mingxing Chu Huihua Wang Caihong Wei |
| author_facet | Qing Li Zengkui Lu Meilin Jin Xiaojuan Fei Kai Quan Yongbin Liu Lin Ma Mingxing Chu Huihua Wang Caihong Wei |
| author_sort | Qing Li |
| collection | DOAJ |
| description | The aim of this study was to examine the correlation between the platelet-derived growth factor-D (<i>PDGF-D</i>) gene and sheep tail type character and explore the potential underlying mechanism. A total of 533 sheep were included in this study. Polymorphic sites were examined by Pool-seq, and individual genotype identification and correlation analysis between tail type data were conducted using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF-MS) method. JASPART website was used to predict transcription factor binding sites in the promoter region with and without <i>PDGF-D</i> gene mutation. The effect of <i>PDGF-D</i> on adipogenic differentiation of sheep preadipocytes was investigated. Two single nucleotide polymorphism sites were identified: g.4122606 C > G site was significantly correlated with tail length, and g.3852134 C > T site was significantly correlated with tail width. g.3852134 C > T was located in the promoter region. Six transcription factor binding sites were eliminated after promoter mutation, and three new transcription factor binding sites appeared. Expression levels of peroxisome proliferator-activated receptor gamma (<i>PPARγ</i>) and lipoproteinlipase (<i>LPL</i>) were significantly up-regulated upon <i>PDGF-D</i> overexpression. Oil red O staining showed increased small and large oil drops in the <i>PDGF-D</i> overexpression group. Together these results indicate the <i>PDGF-D</i> gene is an important gene controlling sheep tail shape and regulating sheep tail fat deposition to a certain degree. |
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| spelling | doaj.art-d6ee6b8345224155a636cbcf06f4b8c92022-12-22T00:59:17ZengMDPI AGAnimals2076-26152020-01-011018910.3390/ani10010089ani10010089Verification and Analysis of Sheep Tail Type-Associated <i>PDGF-D</i> Gene PolymorphismsQing Li0Zengkui Lu1Meilin Jin2Xiaojuan Fei3Kai Quan4Yongbin Liu5Lin Ma6Mingxing Chu7Huihua Wang8Caihong Wei9Key Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaLanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaCollege of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, ChinaInner Mongolia Academy of Animal Husbandry Science, Hohhot 010031, ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaThe aim of this study was to examine the correlation between the platelet-derived growth factor-D (<i>PDGF-D</i>) gene and sheep tail type character and explore the potential underlying mechanism. A total of 533 sheep were included in this study. Polymorphic sites were examined by Pool-seq, and individual genotype identification and correlation analysis between tail type data were conducted using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF-MS) method. JASPART website was used to predict transcription factor binding sites in the promoter region with and without <i>PDGF-D</i> gene mutation. The effect of <i>PDGF-D</i> on adipogenic differentiation of sheep preadipocytes was investigated. Two single nucleotide polymorphism sites were identified: g.4122606 C > G site was significantly correlated with tail length, and g.3852134 C > T site was significantly correlated with tail width. g.3852134 C > T was located in the promoter region. Six transcription factor binding sites were eliminated after promoter mutation, and three new transcription factor binding sites appeared. Expression levels of peroxisome proliferator-activated receptor gamma (<i>PPARγ</i>) and lipoproteinlipase (<i>LPL</i>) were significantly up-regulated upon <i>PDGF-D</i> overexpression. Oil red O staining showed increased small and large oil drops in the <i>PDGF-D</i> overexpression group. Together these results indicate the <i>PDGF-D</i> gene is an important gene controlling sheep tail shape and regulating sheep tail fat deposition to a certain degree.https://www.mdpi.com/2076-2615/10/1/89<i>pdgf-d</i>sheeptail typesnpspreadipocytes |
| spellingShingle | Qing Li Zengkui Lu Meilin Jin Xiaojuan Fei Kai Quan Yongbin Liu Lin Ma Mingxing Chu Huihua Wang Caihong Wei Verification and Analysis of Sheep Tail Type-Associated <i>PDGF-D</i> Gene Polymorphisms Animals <i>pdgf-d</i> sheep tail type snps preadipocytes |
| title | Verification and Analysis of Sheep Tail Type-Associated <i>PDGF-D</i> Gene Polymorphisms |
| title_full | Verification and Analysis of Sheep Tail Type-Associated <i>PDGF-D</i> Gene Polymorphisms |
| title_fullStr | Verification and Analysis of Sheep Tail Type-Associated <i>PDGF-D</i> Gene Polymorphisms |
| title_full_unstemmed | Verification and Analysis of Sheep Tail Type-Associated <i>PDGF-D</i> Gene Polymorphisms |
| title_short | Verification and Analysis of Sheep Tail Type-Associated <i>PDGF-D</i> Gene Polymorphisms |
| title_sort | verification and analysis of sheep tail type associated i pdgf d i gene polymorphisms |
| topic | <i>pdgf-d</i> sheep tail type snps preadipocytes |
| url | https://www.mdpi.com/2076-2615/10/1/89 |
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