Ce-MOF with Intrinsic Haloperoxidase-Like Activity for Ratiometric Colorimetric Detection of Hydrogen Peroxide
Metal–organic framework (MOF) nanozymes, as emerging members of the nanozymes, have received more and more attention due to their composition and structural characteristics. In this work, we report that mixed-valence state Ce-MOF (MVCM) has intrinsic haloperoxidase-mimicking activity. MVCM was synth...
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MDPI AG
2021-06-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/7/204 |
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| author | Yanyan Cheng Ling Liang Fanggui Ye Shulin Zhao |
| author_facet | Yanyan Cheng Ling Liang Fanggui Ye Shulin Zhao |
| author_sort | Yanyan Cheng |
| collection | DOAJ |
| description | Metal–organic framework (MOF) nanozymes, as emerging members of the nanozymes, have received more and more attention due to their composition and structural characteristics. In this work, we report that mixed-valence state Ce-MOF (MVCM) has intrinsic haloperoxidase-mimicking activity. MVCM was synthesized by partial oxidation method using Ce-MOF as a precursor. In the presence of H<sub>2</sub>O<sub>2</sub> and Br<sup>−</sup>, MVCM can catalyze oxidative bromination of chromogenic substrate phenol red (PR) to produce the blue product bromophenol blue (Br<sub>4</sub>PR), showing good haloperoxidase-like activity. Because of the special chromogenic substrate, we constructed a ratiometric colorimetric-sensing platform by detecting the absorbance of the MVCM-(PR, Br<sup>−</sup>) system at wavelengths of 590 and 430, for quantifying H<sub>2</sub>O<sub>2</sub>, where the detection limit of the H<sub>2</sub>O<sub>2</sub> is 3.25 μM. In addition, the haloperoxidase-mimicking mechanism of the MVCM is proposed. Moreover, through enzyme kinetics monitoring, the <i>K<sub>m</sub></i> (H<sub>2</sub>O<sub>2</sub> and NH<sub>4</sub>Br) of the MVCM is lower than that of cerium oxide nanomaterials, indicating that the MVCM has a stronger binding affinity for H<sub>2</sub>O<sub>2</sub> and NH<sub>4</sub>Br than other materials. This work provides more application prospects for the development of nanozymes in the field of biosensors in the future. |
| first_indexed | 2024-03-10T10:08:54Z |
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| id | doaj.art-d6eef5fa7a31449998c0e8bfc812475c |
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| language | English |
| last_indexed | 2024-03-10T10:08:54Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-d6eef5fa7a31449998c0e8bfc812475c2023-11-22T01:19:56ZengMDPI AGBiosensors2079-63742021-06-0111720410.3390/bios11070204Ce-MOF with Intrinsic Haloperoxidase-Like Activity for Ratiometric Colorimetric Detection of Hydrogen PeroxideYanyan Cheng0Ling Liang1Fanggui Ye2Shulin Zhao3State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, ChinaState Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, ChinaState Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, ChinaState Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, ChinaMetal–organic framework (MOF) nanozymes, as emerging members of the nanozymes, have received more and more attention due to their composition and structural characteristics. In this work, we report that mixed-valence state Ce-MOF (MVCM) has intrinsic haloperoxidase-mimicking activity. MVCM was synthesized by partial oxidation method using Ce-MOF as a precursor. In the presence of H<sub>2</sub>O<sub>2</sub> and Br<sup>−</sup>, MVCM can catalyze oxidative bromination of chromogenic substrate phenol red (PR) to produce the blue product bromophenol blue (Br<sub>4</sub>PR), showing good haloperoxidase-like activity. Because of the special chromogenic substrate, we constructed a ratiometric colorimetric-sensing platform by detecting the absorbance of the MVCM-(PR, Br<sup>−</sup>) system at wavelengths of 590 and 430, for quantifying H<sub>2</sub>O<sub>2</sub>, where the detection limit of the H<sub>2</sub>O<sub>2</sub> is 3.25 μM. In addition, the haloperoxidase-mimicking mechanism of the MVCM is proposed. Moreover, through enzyme kinetics monitoring, the <i>K<sub>m</sub></i> (H<sub>2</sub>O<sub>2</sub> and NH<sub>4</sub>Br) of the MVCM is lower than that of cerium oxide nanomaterials, indicating that the MVCM has a stronger binding affinity for H<sub>2</sub>O<sub>2</sub> and NH<sub>4</sub>Br than other materials. This work provides more application prospects for the development of nanozymes in the field of biosensors in the future.https://www.mdpi.com/2079-6374/11/7/204nanozymeCe-MOFhaloperoxidase-like activityratiometric colorimetrichydrogen peroxide |
| spellingShingle | Yanyan Cheng Ling Liang Fanggui Ye Shulin Zhao Ce-MOF with Intrinsic Haloperoxidase-Like Activity for Ratiometric Colorimetric Detection of Hydrogen Peroxide Biosensors nanozyme Ce-MOF haloperoxidase-like activity ratiometric colorimetric hydrogen peroxide |
| title | Ce-MOF with Intrinsic Haloperoxidase-Like Activity for Ratiometric Colorimetric Detection of Hydrogen Peroxide |
| title_full | Ce-MOF with Intrinsic Haloperoxidase-Like Activity for Ratiometric Colorimetric Detection of Hydrogen Peroxide |
| title_fullStr | Ce-MOF with Intrinsic Haloperoxidase-Like Activity for Ratiometric Colorimetric Detection of Hydrogen Peroxide |
| title_full_unstemmed | Ce-MOF with Intrinsic Haloperoxidase-Like Activity for Ratiometric Colorimetric Detection of Hydrogen Peroxide |
| title_short | Ce-MOF with Intrinsic Haloperoxidase-Like Activity for Ratiometric Colorimetric Detection of Hydrogen Peroxide |
| title_sort | ce mof with intrinsic haloperoxidase like activity for ratiometric colorimetric detection of hydrogen peroxide |
| topic | nanozyme Ce-MOF haloperoxidase-like activity ratiometric colorimetric hydrogen peroxide |
| url | https://www.mdpi.com/2079-6374/11/7/204 |
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