Molecular characterization of Clostridium botulinum isolates from foodborne outbreaks in Thailand, 2010.
BACKGROUND: Thailand has had several foodborne outbreaks of botulism, one of the biggest being in 2006 when laboratory investigations identified the etiologic agent as Clostridium botulinum type A. Identification of the etiologic agent from outbreak samples is laborious using conventional microbiolo...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3903786?pdf=render |
| _version_ | 1818928187193688064 |
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| author | Piyada Wangroongsarb Tomoko Kohda Chutima Jittaprasartsin Karun Suthivarakom Thanitchi Kamthalang Kaoru Umeda Pathom Sawanpanyalert Shunji Kozaki Kazuyoshi Ikuta |
| author_facet | Piyada Wangroongsarb Tomoko Kohda Chutima Jittaprasartsin Karun Suthivarakom Thanitchi Kamthalang Kaoru Umeda Pathom Sawanpanyalert Shunji Kozaki Kazuyoshi Ikuta |
| author_sort | Piyada Wangroongsarb |
| collection | DOAJ |
| description | BACKGROUND: Thailand has had several foodborne outbreaks of botulism, one of the biggest being in 2006 when laboratory investigations identified the etiologic agent as Clostridium botulinum type A. Identification of the etiologic agent from outbreak samples is laborious using conventional microbiological methods and the neurotoxin mouse bioassay. Advances in molecular techniques have added enormous information regarding the etiology of outbreaks and characterization of isolates. We applied these methods in three outbreaks of botulism in Thailand in 2010. METHODOLOGY/PRINCIPAL FINDINGS: A total of 19 cases were involved (seven each in Lampang and Saraburi and five in Maehongson provinces). The first outbreak in Lampang province in April 2010 was associated with C. botulinum type F, which was detected by conventional methods. Outbreaks in Saraburi and Maehongson provinces occurred in May and December were due to C. botulinum type A1(B) and B that were identified by conventional methods and molecular techniques, respectively. The result of phylogenetic sequence analysis showed that C. botulinum type A1(B) strain Saraburi 2010 was close to strain Iwate 2007. Molecular analysis of the third outbreak in Maehongson province showed C. botulinum type B8, which was different from B1-B7 subtype. The nontoxic component genes of strain Maehongson 2010 revealed that ha33, ha17 and botR genes were close to strain Okra (B1) while ha70 and ntnh genes were close to strain 111 (B2). CONCLUSION/SIGNIFICANCE: This study demonstrates the utility of molecular genotyping of C. botulinum and how it contributes to our understanding the epidemiology and variation of boNT gene. Thus, the recent botulism outbreaks in Thailand were induced by various C. botulinum types. |
| first_indexed | 2024-12-20T03:24:55Z |
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| id | doaj.art-d6f438f6eda740e292eee1bbd2997ee7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T03:24:55Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-d6f438f6eda740e292eee1bbd2997ee72022-12-21T19:55:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e7779210.1371/journal.pone.0077792Molecular characterization of Clostridium botulinum isolates from foodborne outbreaks in Thailand, 2010.Piyada WangroongsarbTomoko KohdaChutima JittaprasartsinKarun SuthivarakomThanitchi KamthalangKaoru UmedaPathom SawanpanyalertShunji KozakiKazuyoshi IkutaBACKGROUND: Thailand has had several foodborne outbreaks of botulism, one of the biggest being in 2006 when laboratory investigations identified the etiologic agent as Clostridium botulinum type A. Identification of the etiologic agent from outbreak samples is laborious using conventional microbiological methods and the neurotoxin mouse bioassay. Advances in molecular techniques have added enormous information regarding the etiology of outbreaks and characterization of isolates. We applied these methods in three outbreaks of botulism in Thailand in 2010. METHODOLOGY/PRINCIPAL FINDINGS: A total of 19 cases were involved (seven each in Lampang and Saraburi and five in Maehongson provinces). The first outbreak in Lampang province in April 2010 was associated with C. botulinum type F, which was detected by conventional methods. Outbreaks in Saraburi and Maehongson provinces occurred in May and December were due to C. botulinum type A1(B) and B that were identified by conventional methods and molecular techniques, respectively. The result of phylogenetic sequence analysis showed that C. botulinum type A1(B) strain Saraburi 2010 was close to strain Iwate 2007. Molecular analysis of the third outbreak in Maehongson province showed C. botulinum type B8, which was different from B1-B7 subtype. The nontoxic component genes of strain Maehongson 2010 revealed that ha33, ha17 and botR genes were close to strain Okra (B1) while ha70 and ntnh genes were close to strain 111 (B2). CONCLUSION/SIGNIFICANCE: This study demonstrates the utility of molecular genotyping of C. botulinum and how it contributes to our understanding the epidemiology and variation of boNT gene. Thus, the recent botulism outbreaks in Thailand were induced by various C. botulinum types.http://europepmc.org/articles/PMC3903786?pdf=render |
| spellingShingle | Piyada Wangroongsarb Tomoko Kohda Chutima Jittaprasartsin Karun Suthivarakom Thanitchi Kamthalang Kaoru Umeda Pathom Sawanpanyalert Shunji Kozaki Kazuyoshi Ikuta Molecular characterization of Clostridium botulinum isolates from foodborne outbreaks in Thailand, 2010. PLoS ONE |
| title | Molecular characterization of Clostridium botulinum isolates from foodborne outbreaks in Thailand, 2010. |
| title_full | Molecular characterization of Clostridium botulinum isolates from foodborne outbreaks in Thailand, 2010. |
| title_fullStr | Molecular characterization of Clostridium botulinum isolates from foodborne outbreaks in Thailand, 2010. |
| title_full_unstemmed | Molecular characterization of Clostridium botulinum isolates from foodborne outbreaks in Thailand, 2010. |
| title_short | Molecular characterization of Clostridium botulinum isolates from foodborne outbreaks in Thailand, 2010. |
| title_sort | molecular characterization of clostridium botulinum isolates from foodborne outbreaks in thailand 2010 |
| url | http://europepmc.org/articles/PMC3903786?pdf=render |
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