Decreased Steroid Hormone Receptor NR4A2 Expression in Kawasaki Disease Before IVIG Treatment
Kawasaki disease (KD) is anacute febrile coronary vasculitis disease in children. In general, this disease can be treated with a single dose of 2 g/kg intravenous immunoglobulin (IVIG). However, the best timing for administering steroid treatment in acute-stage KD is still under debate. In this stud...
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Pediatrics |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fped.2019.00007/full |
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| author | Ying-Hsien Huang Ying-Hsien Huang Kuang-Den Chen Kuang-Den Chen Mao-Hung Lo Mao-Hung Lo Xin-Yuan Cai Xin-Yuan Cai Ho-Chang Kuo Ho-Chang Kuo |
| author_facet | Ying-Hsien Huang Ying-Hsien Huang Kuang-Den Chen Kuang-Den Chen Mao-Hung Lo Mao-Hung Lo Xin-Yuan Cai Xin-Yuan Cai Ho-Chang Kuo Ho-Chang Kuo |
| author_sort | Ying-Hsien Huang |
| collection | DOAJ |
| description | Kawasaki disease (KD) is anacute febrile coronary vasculitis disease in children. In general, this disease can be treated with a single dose of 2 g/kg intravenous immunoglobulin (IVIG). However, the best timing for administering steroid treatment in acute-stage KD is still under debate. In this study, we recruited 174 participants to survey the transcript levels of steroid hormone receptors in KD patients. The chip studies consisted of 18 KD patients that were analyzed before IVIG treatment and at least 3 weeks after IVIG administration, as well as 36 control subjects, using GeneChip® HTA 2.0. Another cohort consisting of 120 subjects was analyzed to validate qRT-PCR. Our microarray study demonstrated significant downregulated expressions of the mRNA levels of NR1A2, RORA, NR4A1-3, THRA, and PPARD in KD patients in comparision to the controls. However, these genes increased considerably in KD patients after IVIG administration. After PCR validation, our data only revealed decreased NR4A2 mRNA expression in the KD patients compared to those of the controls, which increased after they received IVIG treatment. Our study is the first to report the potential effective utilization of steroid treatment in KD. Prior to IVIG treatment, decreased steroid receptors allowed for the reduced treatment role of steroids. However, after IVIG treatment, increased steroid receptors indicate that steroids are effective as a supplementary treatment for KD. |
| first_indexed | 2024-04-12T03:02:15Z |
| format | Article |
| id | doaj.art-d6f88bbc9f8c4a56ad1a4ff5f60a5d85 |
| institution | Directory Open Access Journal |
| issn | 2296-2360 |
| language | English |
| last_indexed | 2024-04-12T03:02:15Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pediatrics |
| spelling | doaj.art-d6f88bbc9f8c4a56ad1a4ff5f60a5d852022-12-22T03:50:38ZengFrontiers Media S.A.Frontiers in Pediatrics2296-23602019-02-01710.3389/fped.2019.00007435642Decreased Steroid Hormone Receptor NR4A2 Expression in Kawasaki Disease Before IVIG TreatmentYing-Hsien Huang0Ying-Hsien Huang1Kuang-Den Chen2Kuang-Den Chen3Mao-Hung Lo4Mao-Hung Lo5Xin-Yuan Cai6Xin-Yuan Cai7Ho-Chang Kuo8Ho-Chang Kuo9Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, TaiwanKawasaki Disease Center, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, TaiwanKawasaki Disease Center, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, TaiwanDepartment of Surgery, Liver Transplantation Center, Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, TaiwanDepartment of Pediatrics, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, TaiwanKawasaki Disease Center, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, TaiwanDepartment of Pediatrics, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, TaiwanKawasaki Disease Center, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, TaiwanDepartment of Pediatrics, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, TaiwanKawasaki Disease Center, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, TaiwanKawasaki disease (KD) is anacute febrile coronary vasculitis disease in children. In general, this disease can be treated with a single dose of 2 g/kg intravenous immunoglobulin (IVIG). However, the best timing for administering steroid treatment in acute-stage KD is still under debate. In this study, we recruited 174 participants to survey the transcript levels of steroid hormone receptors in KD patients. The chip studies consisted of 18 KD patients that were analyzed before IVIG treatment and at least 3 weeks after IVIG administration, as well as 36 control subjects, using GeneChip® HTA 2.0. Another cohort consisting of 120 subjects was analyzed to validate qRT-PCR. Our microarray study demonstrated significant downregulated expressions of the mRNA levels of NR1A2, RORA, NR4A1-3, THRA, and PPARD in KD patients in comparision to the controls. However, these genes increased considerably in KD patients after IVIG administration. After PCR validation, our data only revealed decreased NR4A2 mRNA expression in the KD patients compared to those of the controls, which increased after they received IVIG treatment. Our study is the first to report the potential effective utilization of steroid treatment in KD. Prior to IVIG treatment, decreased steroid receptors allowed for the reduced treatment role of steroids. However, after IVIG treatment, increased steroid receptors indicate that steroids are effective as a supplementary treatment for KD.https://www.frontiersin.org/article/10.3389/fped.2019.00007/fullKawasaki disease (KD)steroid receptorNr4a2immunologyIVIG = intravenous immunoglobulin |
| spellingShingle | Ying-Hsien Huang Ying-Hsien Huang Kuang-Den Chen Kuang-Den Chen Mao-Hung Lo Mao-Hung Lo Xin-Yuan Cai Xin-Yuan Cai Ho-Chang Kuo Ho-Chang Kuo Decreased Steroid Hormone Receptor NR4A2 Expression in Kawasaki Disease Before IVIG Treatment Frontiers in Pediatrics Kawasaki disease (KD) steroid receptor Nr4a2 immunology IVIG = intravenous immunoglobulin |
| title | Decreased Steroid Hormone Receptor NR4A2 Expression in Kawasaki Disease Before IVIG Treatment |
| title_full | Decreased Steroid Hormone Receptor NR4A2 Expression in Kawasaki Disease Before IVIG Treatment |
| title_fullStr | Decreased Steroid Hormone Receptor NR4A2 Expression in Kawasaki Disease Before IVIG Treatment |
| title_full_unstemmed | Decreased Steroid Hormone Receptor NR4A2 Expression in Kawasaki Disease Before IVIG Treatment |
| title_short | Decreased Steroid Hormone Receptor NR4A2 Expression in Kawasaki Disease Before IVIG Treatment |
| title_sort | decreased steroid hormone receptor nr4a2 expression in kawasaki disease before ivig treatment |
| topic | Kawasaki disease (KD) steroid receptor Nr4a2 immunology IVIG = intravenous immunoglobulin |
| url | https://www.frontiersin.org/article/10.3389/fped.2019.00007/full |
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