Isolation and bioinformatics analysis of the NADC30_Like CJS01 strain of the porcine reproductive and respiratory syndrome virus
In this study, lung tissue was collected from nursery piglets suspected of being infected with porcine reproductive and respiratory syndrome virus (PRRSV) in a large‑scale pig farm in Sichuan, China. Polymerase chain reaction (PCR) and reverse transcription quantitative‑polymerase chain reaction (R...
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| Format: | Article |
| Language: | English |
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Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2022-11-01
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| Series: | Veterinaria Italiana |
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| Online Access: | https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/2182 |
| _version_ | 1811186645599256576 |
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| author | TENG TU YIN WANG CHANG YU LIAO PENG FEI ZHANG MING YUAN XIANG ZE XIAO YANG XUE PING YAO YAN LUO |
| author_facet | TENG TU YIN WANG CHANG YU LIAO PENG FEI ZHANG MING YUAN XIANG ZE XIAO YANG XUE PING YAO YAN LUO |
| author_sort | TENG TU |
| collection | DOAJ |
| description |
In this study, lung tissue was collected from nursery piglets suspected of being infected with porcine reproductive and respiratory syndrome virus (PRRSV) in a large‑scale pig farm in Sichuan, China. Polymerase chain reaction (PCR) and reverse transcription quantitative‑polymerase chain reaction (RT‑qPCR) methods were used to ensure that no other pathogens were present. Virus isolation was also carried out where the presence of PRRSV was determined by indirect immunoinfluscent assay (IFA). Compared with the common PRRSV strain, the isolate did not produce evident Cytopathic effect (CPE) in the early stage of isolation. CPE was found in the late stage, and the titer was 104.17 TCID50/0.1 mL. The strain was named CJS01. Bioinformatics analysis showed that it was a NADC30‑Like strain. The virus load was determined by measuring the nucleic acid load during the proliferation of the strain on Marc‑145 cells. The strain showed good adaptability on cells, and the virus proliferated on cells for 84 hr when the highest nucleic acid load was achieved. By recombinant analysis of ORF3~7 genes and prediction of its epitope, it was found that CJS01 strain might interfere with the immunesystem of the infected animals.
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| first_indexed | 2024-04-11T13:49:35Z |
| format | Article |
| id | doaj.art-d6f9f1798521412ebd40c28397bb2579 |
| institution | Directory Open Access Journal |
| issn | 0505-401X 1828-1427 |
| language | English |
| last_indexed | 2024-04-11T13:49:35Z |
| publishDate | 2022-11-01 |
| publisher | Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale |
| record_format | Article |
| series | Veterinaria Italiana |
| spelling | doaj.art-d6f9f1798521412ebd40c28397bb25792022-12-22T04:20:46ZengIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. CaporaleVeterinaria Italiana0505-401X1828-14272022-11-0158110.12834/VetIt.2182.14564.1Isolation and bioinformatics analysis of the NADC30_Like CJS01 strain of the porcine reproductive and respiratory syndrome virusTENG TUYIN WANGCHANG YU LIAOPENG FEI ZHANGMING YUAN XIANGZE XIAO YANGXUE PING YAOYAN LUO In this study, lung tissue was collected from nursery piglets suspected of being infected with porcine reproductive and respiratory syndrome virus (PRRSV) in a large‑scale pig farm in Sichuan, China. Polymerase chain reaction (PCR) and reverse transcription quantitative‑polymerase chain reaction (RT‑qPCR) methods were used to ensure that no other pathogens were present. Virus isolation was also carried out where the presence of PRRSV was determined by indirect immunoinfluscent assay (IFA). Compared with the common PRRSV strain, the isolate did not produce evident Cytopathic effect (CPE) in the early stage of isolation. CPE was found in the late stage, and the titer was 104.17 TCID50/0.1 mL. The strain was named CJS01. Bioinformatics analysis showed that it was a NADC30‑Like strain. The virus load was determined by measuring the nucleic acid load during the proliferation of the strain on Marc‑145 cells. The strain showed good adaptability on cells, and the virus proliferated on cells for 84 hr when the highest nucleic acid load was achieved. By recombinant analysis of ORF3~7 genes and prediction of its epitope, it was found that CJS01 strain might interfere with the immunesystem of the infected animals. https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/2182Bioinformatics analysisIsolationPRRSV NADC30‑Like |
| spellingShingle | TENG TU YIN WANG CHANG YU LIAO PENG FEI ZHANG MING YUAN XIANG ZE XIAO YANG XUE PING YAO YAN LUO Isolation and bioinformatics analysis of the NADC30_Like CJS01 strain of the porcine reproductive and respiratory syndrome virus Veterinaria Italiana Bioinformatics analysis Isolation PRRSV NADC30‑Like |
| title | Isolation and bioinformatics analysis of the NADC30_Like CJS01 strain of the porcine reproductive and respiratory syndrome virus |
| title_full | Isolation and bioinformatics analysis of the NADC30_Like CJS01 strain of the porcine reproductive and respiratory syndrome virus |
| title_fullStr | Isolation and bioinformatics analysis of the NADC30_Like CJS01 strain of the porcine reproductive and respiratory syndrome virus |
| title_full_unstemmed | Isolation and bioinformatics analysis of the NADC30_Like CJS01 strain of the porcine reproductive and respiratory syndrome virus |
| title_short | Isolation and bioinformatics analysis of the NADC30_Like CJS01 strain of the porcine reproductive and respiratory syndrome virus |
| title_sort | isolation and bioinformatics analysis of the nadc30 like cjs01 strain of the porcine reproductive and respiratory syndrome virus |
| topic | Bioinformatics analysis Isolation PRRSV NADC30‑Like |
| url | https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/2182 |
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