Investigation of Potency and Safety of Live-Attenuated Peste des Petits Ruminant Virus Vaccine in Goats by Detection of Cellular and Humoral Immune Response
The peste des petits ruminant (PPR) virus is a transboundary virus found in small domestic ruminants that causes high morbidity and mortality in naive herds. PPR can be effectively controlled and eradicated by vaccinating small domestic ruminants with a live-attenuated peste des petits ruminant viru...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/6/1325 |
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| author | Milovan Milovanović Klaas Dietze Ulrich Wernery Bernd Hoffmann |
| author_facet | Milovan Milovanović Klaas Dietze Ulrich Wernery Bernd Hoffmann |
| author_sort | Milovan Milovanović |
| collection | DOAJ |
| description | The peste des petits ruminant (PPR) virus is a transboundary virus found in small domestic ruminants that causes high morbidity and mortality in naive herds. PPR can be effectively controlled and eradicated by vaccinating small domestic ruminants with a live-attenuated peste des petits ruminant virus (PPRV) vaccine, which provides long-lasting immunity. We studied the potency and safety of a live-attenuated vaccine in goats by detecting their cellular and humoral immune responses. Six goats were subcutaneously vaccinated with a live-attenuated PPRV vaccine according to the manufacturer’s instructions, and two goats were kept in contact. Following vaccination, the goats were monitored daily, and we recorded their body temperature and clinical score. Heparinized blood and serum were collected for a serological analysis, and swab samples and EDTA blood were collected to detect the PPRV genome. The safety of the used PPRV vaccine was confirmed by the absence of PPR-related clinical signs, a negative pen-side test, a low virus genome load as detected with RT-qPCR on the vaccinated goats, and the lack horizontal transmission between the in-contact goats. The strong humoral and cellular immune responses detected in the vaccinated goats showed that the live-attenuated PPRV vaccine has a strong potency in goats. Therefore, live-attenuated vaccines against PPR can be used to control and eradicate PRR. |
| first_indexed | 2024-03-11T01:50:27Z |
| format | Article |
| id | doaj.art-d6fb5cec73ce4601aa958597a99a9a0f |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T01:50:27Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-d6fb5cec73ce4601aa958597a99a9a0f2023-11-18T13:02:13ZengMDPI AGViruses1999-49152023-06-01156132510.3390/v15061325Investigation of Potency and Safety of Live-Attenuated Peste des Petits Ruminant Virus Vaccine in Goats by Detection of Cellular and Humoral Immune ResponseMilovan Milovanović0Klaas Dietze1Ulrich Wernery2Bernd Hoffmann3Friedrich-Loeffler-Institut, Südufer 10, 17943 Greifswald-Insel Riems, GermanyFriedrich-Loeffler-Institut, Südufer 10, 17943 Greifswald-Insel Riems, GermanyCentral Veterinary Research Laboratory, Dubai P.O. Box 597, United Arab EmiratesFriedrich-Loeffler-Institut, Südufer 10, 17943 Greifswald-Insel Riems, GermanyThe peste des petits ruminant (PPR) virus is a transboundary virus found in small domestic ruminants that causes high morbidity and mortality in naive herds. PPR can be effectively controlled and eradicated by vaccinating small domestic ruminants with a live-attenuated peste des petits ruminant virus (PPRV) vaccine, which provides long-lasting immunity. We studied the potency and safety of a live-attenuated vaccine in goats by detecting their cellular and humoral immune responses. Six goats were subcutaneously vaccinated with a live-attenuated PPRV vaccine according to the manufacturer’s instructions, and two goats were kept in contact. Following vaccination, the goats were monitored daily, and we recorded their body temperature and clinical score. Heparinized blood and serum were collected for a serological analysis, and swab samples and EDTA blood were collected to detect the PPRV genome. The safety of the used PPRV vaccine was confirmed by the absence of PPR-related clinical signs, a negative pen-side test, a low virus genome load as detected with RT-qPCR on the vaccinated goats, and the lack horizontal transmission between the in-contact goats. The strong humoral and cellular immune responses detected in the vaccinated goats showed that the live-attenuated PPRV vaccine has a strong potency in goats. Therefore, live-attenuated vaccines against PPR can be used to control and eradicate PRR.https://www.mdpi.com/1999-4915/15/6/1325PPRPPRVgoatslive-attenuated vaccineIFN-γPPRV specific antibodies |
| spellingShingle | Milovan Milovanović Klaas Dietze Ulrich Wernery Bernd Hoffmann Investigation of Potency and Safety of Live-Attenuated Peste des Petits Ruminant Virus Vaccine in Goats by Detection of Cellular and Humoral Immune Response Viruses PPR PPRV goats live-attenuated vaccine IFN-γ PPRV specific antibodies |
| title | Investigation of Potency and Safety of Live-Attenuated Peste des Petits Ruminant Virus Vaccine in Goats by Detection of Cellular and Humoral Immune Response |
| title_full | Investigation of Potency and Safety of Live-Attenuated Peste des Petits Ruminant Virus Vaccine in Goats by Detection of Cellular and Humoral Immune Response |
| title_fullStr | Investigation of Potency and Safety of Live-Attenuated Peste des Petits Ruminant Virus Vaccine in Goats by Detection of Cellular and Humoral Immune Response |
| title_full_unstemmed | Investigation of Potency and Safety of Live-Attenuated Peste des Petits Ruminant Virus Vaccine in Goats by Detection of Cellular and Humoral Immune Response |
| title_short | Investigation of Potency and Safety of Live-Attenuated Peste des Petits Ruminant Virus Vaccine in Goats by Detection of Cellular and Humoral Immune Response |
| title_sort | investigation of potency and safety of live attenuated peste des petits ruminant virus vaccine in goats by detection of cellular and humoral immune response |
| topic | PPR PPRV goats live-attenuated vaccine IFN-γ PPRV specific antibodies |
| url | https://www.mdpi.com/1999-4915/15/6/1325 |
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