Antioxidant and Antimutagenic Activities of Different Fractions from the Leaves of Rhododendron arboreum Sm. and Their GC-MS Profiling
In this era of urbanization and environmental pollution, antioxidants and antimutagens derived from plants are promising safeguards for human health. In the current investigation, we analyzed the antioxidant and antimutagenic effects of the hexane, chloroform, and ethyl acetate fractions of Rhododen...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-09-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/23/9/2239 |
| _version_ | 1818067655820574720 |
|---|---|
| author | Vandana Gautam Sukhmeen Kaur Kohli Saroj Arora Renu Bhardwaj Mohsin Kazi Ajaz Ahmad Mohammad Raish Majid Ahmad Ganaie Parvaiz Ahmad |
| author_facet | Vandana Gautam Sukhmeen Kaur Kohli Saroj Arora Renu Bhardwaj Mohsin Kazi Ajaz Ahmad Mohammad Raish Majid Ahmad Ganaie Parvaiz Ahmad |
| author_sort | Vandana Gautam |
| collection | DOAJ |
| description | In this era of urbanization and environmental pollution, antioxidants and antimutagens derived from plants are promising safeguards for human health. In the current investigation, we analyzed the antioxidant and antimutagenic effects of the hexane, chloroform, and ethyl acetate fractions of Rhododendron arboreum Sm. leaves and determined their chemical composition. The different fractions inhibited lipid peroxidation, repressed the production of nitric oxide radicals, and prevented deoxyribose degradation. The antimutagenic activity of the leaf fractions was analyzed against 4-nitro-O-phenylenediamine, sodium azide and 2-aminofluorene mutagens in two test strains (TA-98 and TA-100) of Salmonella typhimurium. The experiment was conducted using pre- and co-incubation modes. The best results were obtained in the pre-incubation mode, and against indirect acting mutagen. The presence of a number of bioactive constituents was confirmed in the different fractions by GC-MS analysis. The study reveals the strong antioxidant and antimutagenic activity of R. arboreum leaves. We propose that those activities of R. arboreum might correspond to the combined effect of the phytochemicals identified by GC-MS analysis. To the best of our knowledge, this is the first report on the antimutagenic activity of R. arboreum leaves. |
| first_indexed | 2024-12-10T15:27:08Z |
| format | Article |
| id | doaj.art-d701c6f44f274a19af56fae8ca6a746d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T15:27:08Z |
| publishDate | 2018-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d701c6f44f274a19af56fae8ca6a746d2022-12-22T01:43:31ZengMDPI AGMolecules1420-30492018-09-01239223910.3390/molecules23092239molecules23092239Antioxidant and Antimutagenic Activities of Different Fractions from the Leaves of Rhododendron arboreum Sm. and Their GC-MS ProfilingVandana Gautam0Sukhmeen Kaur Kohli1Saroj Arora2Renu Bhardwaj3Mohsin Kazi4Ajaz Ahmad5Mohammad Raish6Majid Ahmad Ganaie7Parvaiz Ahmad8Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, Punjab, IndiaDepartment of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, Punjab, IndiaDepartment of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, Punjab, IndiaDepartment of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, Punjab, IndiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmacology, College of Pharmacy, Prince Sattam bin Abdulaziz University, Alkharaj 11942, Saudi ArabiaDepartment of Botany and Microbiology, Faculty of Science, King Saud University, Riyadh 11451, Saudi ArabiaIn this era of urbanization and environmental pollution, antioxidants and antimutagens derived from plants are promising safeguards for human health. In the current investigation, we analyzed the antioxidant and antimutagenic effects of the hexane, chloroform, and ethyl acetate fractions of Rhododendron arboreum Sm. leaves and determined their chemical composition. The different fractions inhibited lipid peroxidation, repressed the production of nitric oxide radicals, and prevented deoxyribose degradation. The antimutagenic activity of the leaf fractions was analyzed against 4-nitro-O-phenylenediamine, sodium azide and 2-aminofluorene mutagens in two test strains (TA-98 and TA-100) of Salmonella typhimurium. The experiment was conducted using pre- and co-incubation modes. The best results were obtained in the pre-incubation mode, and against indirect acting mutagen. The presence of a number of bioactive constituents was confirmed in the different fractions by GC-MS analysis. The study reveals the strong antioxidant and antimutagenic activity of R. arboreum leaves. We propose that those activities of R. arboreum might correspond to the combined effect of the phytochemicals identified by GC-MS analysis. To the best of our knowledge, this is the first report on the antimutagenic activity of R. arboreum leaves.http://www.mdpi.com/1420-3049/23/9/2239antimutagenic activityAmes assayGC-MSRhododendron arboreum Sm.chemical compositionantioxidant activity |
| spellingShingle | Vandana Gautam Sukhmeen Kaur Kohli Saroj Arora Renu Bhardwaj Mohsin Kazi Ajaz Ahmad Mohammad Raish Majid Ahmad Ganaie Parvaiz Ahmad Antioxidant and Antimutagenic Activities of Different Fractions from the Leaves of Rhododendron arboreum Sm. and Their GC-MS Profiling Molecules antimutagenic activity Ames assay GC-MS Rhododendron arboreum Sm. chemical composition antioxidant activity |
| title | Antioxidant and Antimutagenic Activities of Different Fractions from the Leaves of Rhododendron arboreum Sm. and Their GC-MS Profiling |
| title_full | Antioxidant and Antimutagenic Activities of Different Fractions from the Leaves of Rhododendron arboreum Sm. and Their GC-MS Profiling |
| title_fullStr | Antioxidant and Antimutagenic Activities of Different Fractions from the Leaves of Rhododendron arboreum Sm. and Their GC-MS Profiling |
| title_full_unstemmed | Antioxidant and Antimutagenic Activities of Different Fractions from the Leaves of Rhododendron arboreum Sm. and Their GC-MS Profiling |
| title_short | Antioxidant and Antimutagenic Activities of Different Fractions from the Leaves of Rhododendron arboreum Sm. and Their GC-MS Profiling |
| title_sort | antioxidant and antimutagenic activities of different fractions from the leaves of rhododendron arboreum sm and their gc ms profiling |
| topic | antimutagenic activity Ames assay GC-MS Rhododendron arboreum Sm. chemical composition antioxidant activity |
| url | http://www.mdpi.com/1420-3049/23/9/2239 |
| work_keys_str_mv | AT vandanagautam antioxidantandantimutagenicactivitiesofdifferentfractionsfromtheleavesofrhododendronarboreumsmandtheirgcmsprofiling AT sukhmeenkaurkohli antioxidantandantimutagenicactivitiesofdifferentfractionsfromtheleavesofrhododendronarboreumsmandtheirgcmsprofiling AT sarojarora antioxidantandantimutagenicactivitiesofdifferentfractionsfromtheleavesofrhododendronarboreumsmandtheirgcmsprofiling AT renubhardwaj antioxidantandantimutagenicactivitiesofdifferentfractionsfromtheleavesofrhododendronarboreumsmandtheirgcmsprofiling AT mohsinkazi antioxidantandantimutagenicactivitiesofdifferentfractionsfromtheleavesofrhododendronarboreumsmandtheirgcmsprofiling AT ajazahmad antioxidantandantimutagenicactivitiesofdifferentfractionsfromtheleavesofrhododendronarboreumsmandtheirgcmsprofiling AT mohammadraish antioxidantandantimutagenicactivitiesofdifferentfractionsfromtheleavesofrhododendronarboreumsmandtheirgcmsprofiling AT majidahmadganaie antioxidantandantimutagenicactivitiesofdifferentfractionsfromtheleavesofrhododendronarboreumsmandtheirgcmsprofiling AT parvaizahmad antioxidantandantimutagenicactivitiesofdifferentfractionsfromtheleavesofrhododendronarboreumsmandtheirgcmsprofiling |