Preliminary study on early diagnosis of Alzheimer’s disease in APP/PS1 transgenic mice using multimodal magnetic resonance imaging
Alzheimer’s disease (AD) has an insidious onset and lacks clear early diagnostic markers, and by the time overt dementia symptoms appear, the disease is already in the mid-to-late stages. The search for early diagnostic markers of AD may open a critical window for Alzheimer’s treatment and facilitat...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Aging Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnagi.2024.1326394/full |
| _version_ | 1797311678041292800 |
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| author | Meng Xu Meng Xu Jipeng Liu Qingguo Liu Yu Gong Yinyin Li Yinyin Li Jing Zhang Shufeng Shi Yuanyuan Shi Yuanyuan Shi Yuanyuan Shi |
| author_facet | Meng Xu Meng Xu Jipeng Liu Qingguo Liu Yu Gong Yinyin Li Yinyin Li Jing Zhang Shufeng Shi Yuanyuan Shi Yuanyuan Shi Yuanyuan Shi |
| author_sort | Meng Xu |
| collection | DOAJ |
| description | Alzheimer’s disease (AD) has an insidious onset and lacks clear early diagnostic markers, and by the time overt dementia symptoms appear, the disease is already in the mid-to-late stages. The search for early diagnostic markers of AD may open a critical window for Alzheimer’s treatment and facilitate early intervention to slow the progression of AD. In this study, we aimed to explore the imaging markers for early diagnosis of AD through the combined application of structural magnetic resonance imaging (sMRI), resting-state functional magnetic resonance imaging (rs-fMRI), and 1H-magnetic resonance spectroscopy (1H-MRS) multimodal magnetic resonance imaging (MRI) techniques at the animal experimental level, with the aim to provide a certain reference for early clinical diagnosis of AD. First, sMRI scans were performed on 4-month-old amyloid beta precursor protein/presenilin 1 (APP/PS1) transgenic AD model mice and wild type mice of the same litter using a 7.0 T animal MRI scanner to analyze the differential brain regions with structural changes in the gray matter of the brain by voxel-based morphometry (VBM). Next, rs-fMRI scans were performed to analyze the differential brain regions between groups for local spontaneous brain activity and functional connectivity (FC) between brain regions. Finally, 1H-MRS scans were performed to quantify and analyze intergroup differences in the relative concentrations of different metabolites within regions of interest (cortex and hippocampus). Compared with wild type mice, the volume of the left hippocampus, and right olfactory bulb of APP/PS1 transgenic AD model mice were reduced, the functional activity of the bilateral hippocampus, right piriform cortex and right caudate putamen was reduced, the functional network connectivity of the hippocampus was impaired, and the relative content of N-acetylaspartate (NAA)in the hippocampus was decreased. In addition, this study found that imaging changes in olfactory-related brain regions were closely associated with AD diagnosis, and these findings may provide some reference for the early diagnosis of AD. |
| first_indexed | 2024-03-08T02:03:10Z |
| format | Article |
| id | doaj.art-d703ff6f867b459fb7b97c0ca8d0bcd8 |
| institution | Directory Open Access Journal |
| issn | 1663-4365 |
| language | English |
| last_indexed | 2024-03-08T02:03:10Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Aging Neuroscience |
| spelling | doaj.art-d703ff6f867b459fb7b97c0ca8d0bcd82024-02-14T04:41:35ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652024-02-011610.3389/fnagi.2024.13263941326394Preliminary study on early diagnosis of Alzheimer’s disease in APP/PS1 transgenic mice using multimodal magnetic resonance imagingMeng Xu0Meng Xu1Jipeng Liu2Qingguo Liu3Yu Gong4Yinyin Li5Yinyin Li6Jing Zhang7Shufeng Shi8Yuanyuan Shi9Yuanyuan Shi10Yuanyuan Shi11Department of Tuina, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, ChinaSchool of Life Sciences, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaDepartment Shenzhen Hospital (Longgang), Beijing University of Chinese Medicine, Shenzhen, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Tuina, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, ChinaSchool of Life Sciences, Beijing University of Chinese Medicine, Beijing, ChinaShenzhen Research Institute, Beijing University of Chinese Medicine, Shenzhen, ChinaShenzhen Cell Valley Biopharmaceuticals Co., Ltd., Shenzhen, ChinaAlzheimer’s disease (AD) has an insidious onset and lacks clear early diagnostic markers, and by the time overt dementia symptoms appear, the disease is already in the mid-to-late stages. The search for early diagnostic markers of AD may open a critical window for Alzheimer’s treatment and facilitate early intervention to slow the progression of AD. In this study, we aimed to explore the imaging markers for early diagnosis of AD through the combined application of structural magnetic resonance imaging (sMRI), resting-state functional magnetic resonance imaging (rs-fMRI), and 1H-magnetic resonance spectroscopy (1H-MRS) multimodal magnetic resonance imaging (MRI) techniques at the animal experimental level, with the aim to provide a certain reference for early clinical diagnosis of AD. First, sMRI scans were performed on 4-month-old amyloid beta precursor protein/presenilin 1 (APP/PS1) transgenic AD model mice and wild type mice of the same litter using a 7.0 T animal MRI scanner to analyze the differential brain regions with structural changes in the gray matter of the brain by voxel-based morphometry (VBM). Next, rs-fMRI scans were performed to analyze the differential brain regions between groups for local spontaneous brain activity and functional connectivity (FC) between brain regions. Finally, 1H-MRS scans were performed to quantify and analyze intergroup differences in the relative concentrations of different metabolites within regions of interest (cortex and hippocampus). Compared with wild type mice, the volume of the left hippocampus, and right olfactory bulb of APP/PS1 transgenic AD model mice were reduced, the functional activity of the bilateral hippocampus, right piriform cortex and right caudate putamen was reduced, the functional network connectivity of the hippocampus was impaired, and the relative content of N-acetylaspartate (NAA)in the hippocampus was decreased. In addition, this study found that imaging changes in olfactory-related brain regions were closely associated with AD diagnosis, and these findings may provide some reference for the early diagnosis of AD.https://www.frontiersin.org/articles/10.3389/fnagi.2024.1326394/fullAlzheimer’s diseaseAPP/PS1 transgenic miceearly diagnosissMRIrs-fMRI1H-MRS |
| spellingShingle | Meng Xu Meng Xu Jipeng Liu Qingguo Liu Yu Gong Yinyin Li Yinyin Li Jing Zhang Shufeng Shi Yuanyuan Shi Yuanyuan Shi Yuanyuan Shi Preliminary study on early diagnosis of Alzheimer’s disease in APP/PS1 transgenic mice using multimodal magnetic resonance imaging Frontiers in Aging Neuroscience Alzheimer’s disease APP/PS1 transgenic mice early diagnosis sMRI rs-fMRI 1H-MRS |
| title | Preliminary study on early diagnosis of Alzheimer’s disease in APP/PS1 transgenic mice using multimodal magnetic resonance imaging |
| title_full | Preliminary study on early diagnosis of Alzheimer’s disease in APP/PS1 transgenic mice using multimodal magnetic resonance imaging |
| title_fullStr | Preliminary study on early diagnosis of Alzheimer’s disease in APP/PS1 transgenic mice using multimodal magnetic resonance imaging |
| title_full_unstemmed | Preliminary study on early diagnosis of Alzheimer’s disease in APP/PS1 transgenic mice using multimodal magnetic resonance imaging |
| title_short | Preliminary study on early diagnosis of Alzheimer’s disease in APP/PS1 transgenic mice using multimodal magnetic resonance imaging |
| title_sort | preliminary study on early diagnosis of alzheimer s disease in app ps1 transgenic mice using multimodal magnetic resonance imaging |
| topic | Alzheimer’s disease APP/PS1 transgenic mice early diagnosis sMRI rs-fMRI 1H-MRS |
| url | https://www.frontiersin.org/articles/10.3389/fnagi.2024.1326394/full |
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