Expression of Transcription Factor ZBTB20 in the Adult Primate Neurogenic Niche under Physiological Conditions or after Ischemia
The <i>Zbtb20</i> gene encodes for a transcription factor that plays an important role in mammalian cortical development. Recently, its expression was reported in the adult mouse subventricular zone (SVZ), a major neurogenic niche containing neural stem cells throughout life. Here, we an...
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MDPI AG
2022-08-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/9/1559 |
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| author | Dimo S. Stoyanov Martin N. Ivanov Tetsumori Yamashima Anton B. Tonchev |
| author_facet | Dimo S. Stoyanov Martin N. Ivanov Tetsumori Yamashima Anton B. Tonchev |
| author_sort | Dimo S. Stoyanov |
| collection | DOAJ |
| description | The <i>Zbtb20</i> gene encodes for a transcription factor that plays an important role in mammalian cortical development. Recently, its expression was reported in the adult mouse subventricular zone (SVZ), a major neurogenic niche containing neural stem cells throughout life. Here, we analyzed its expression in the adult primate anterior SVZ (SVZa) and rostral migratory stream (RMS) using macaque monkeys (<i>M</i><i>acaca fuscata</i>). We report that the majority of Ki67+ cells, 71.4% in the SVZa and 85.7% in the RMS, co-label for ZBTB20. Nearly all neuroblasts, identified by their Doublecortin expression, were positive for ZBTB20 in both regions. Nearly all GFAP+ neural stem cells/astrocytes were also positive for ZBTB20. Analysis of images derived from a public database of gene expression in control/ischemic monkey SVZa, showed evidence for <i>ZBTB20</i> upregulation in postischemic monkey SVZa. Furthermore, the co-localization of ZBTB20 with Doublecortin and Ki67 was increased in the postischemic SVZa. Our results suggest that ZBTB20 expression is evolutionarily conserved in the mammalian neurogenic niche and is reactive to ischemia. This opens the possibility for further functional studies on the role of this transcription factor in neurogenesis in primates. |
| first_indexed | 2024-03-09T23:56:53Z |
| format | Article |
| id | doaj.art-d706135fbe054c8e920ae33a5cff4411 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T23:56:53Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-d706135fbe054c8e920ae33a5cff44112023-11-23T16:24:09ZengMDPI AGGenes2073-44252022-08-01139155910.3390/genes13091559Expression of Transcription Factor ZBTB20 in the Adult Primate Neurogenic Niche under Physiological Conditions or after IschemiaDimo S. Stoyanov0Martin N. Ivanov1Tetsumori Yamashima2Anton B. Tonchev3Department of Anatomy and Cell Biology, Faculty of Medicine, Medical University, 9000 Varna, BulgariaDepartment of Anatomy and Cell Biology, Faculty of Medicine, Medical University, 9000 Varna, BulgariaDepartment of Psychiatry and Behavioral Science, Kanazawa University Graduate School of Medical Sciences, Kanazawa 920-1192, JapanDepartment of Anatomy and Cell Biology, Faculty of Medicine, Medical University, 9000 Varna, BulgariaThe <i>Zbtb20</i> gene encodes for a transcription factor that plays an important role in mammalian cortical development. Recently, its expression was reported in the adult mouse subventricular zone (SVZ), a major neurogenic niche containing neural stem cells throughout life. Here, we analyzed its expression in the adult primate anterior SVZ (SVZa) and rostral migratory stream (RMS) using macaque monkeys (<i>M</i><i>acaca fuscata</i>). We report that the majority of Ki67+ cells, 71.4% in the SVZa and 85.7% in the RMS, co-label for ZBTB20. Nearly all neuroblasts, identified by their Doublecortin expression, were positive for ZBTB20 in both regions. Nearly all GFAP+ neural stem cells/astrocytes were also positive for ZBTB20. Analysis of images derived from a public database of gene expression in control/ischemic monkey SVZa, showed evidence for <i>ZBTB20</i> upregulation in postischemic monkey SVZa. Furthermore, the co-localization of ZBTB20 with Doublecortin and Ki67 was increased in the postischemic SVZa. Our results suggest that ZBTB20 expression is evolutionarily conserved in the mammalian neurogenic niche and is reactive to ischemia. This opens the possibility for further functional studies on the role of this transcription factor in neurogenesis in primates.https://www.mdpi.com/2073-4425/13/9/1559ZBTB20non-human primatesubventricular zoneneural progenitor |
| spellingShingle | Dimo S. Stoyanov Martin N. Ivanov Tetsumori Yamashima Anton B. Tonchev Expression of Transcription Factor ZBTB20 in the Adult Primate Neurogenic Niche under Physiological Conditions or after Ischemia Genes ZBTB20 non-human primate subventricular zone neural progenitor |
| title | Expression of Transcription Factor ZBTB20 in the Adult Primate Neurogenic Niche under Physiological Conditions or after Ischemia |
| title_full | Expression of Transcription Factor ZBTB20 in the Adult Primate Neurogenic Niche under Physiological Conditions or after Ischemia |
| title_fullStr | Expression of Transcription Factor ZBTB20 in the Adult Primate Neurogenic Niche under Physiological Conditions or after Ischemia |
| title_full_unstemmed | Expression of Transcription Factor ZBTB20 in the Adult Primate Neurogenic Niche under Physiological Conditions or after Ischemia |
| title_short | Expression of Transcription Factor ZBTB20 in the Adult Primate Neurogenic Niche under Physiological Conditions or after Ischemia |
| title_sort | expression of transcription factor zbtb20 in the adult primate neurogenic niche under physiological conditions or after ischemia |
| topic | ZBTB20 non-human primate subventricular zone neural progenitor |
| url | https://www.mdpi.com/2073-4425/13/9/1559 |
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