Phenotypic and Genotypic Characterization of Streptococci Associated with Clinical Bovine Mastitis
This study was carried out on a total of 550 lactating animals; 310 and 240 cows and buffaloes, respectively which were examined for signs of clinical mastitis (swelling, hotness, redness, and apparent milk change) from different dairy farms and veterinary units located at El-Fayoum Governorate duri...
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Beni-Suef University
2022-04-01
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| Series: | Journal of Veterinary Medical Research |
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| Online Access: | https://jvmr.journals.ekb.eg/article_229626_ad1627362ddfcdb4f281935facda324e.pdf |
| _version_ | 1817990940168552448 |
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| author | Ismail Raheel Asmaa Nady Mohammed Asmaa AbdRabo Mohamed |
| author_facet | Ismail Raheel Asmaa Nady Mohammed Asmaa AbdRabo Mohamed |
| author_sort | Ismail Raheel |
| collection | DOAJ |
| description | This study was carried out on a total of 550 lactating animals; 310 and 240 cows and buffaloes, respectively which were examined for signs of clinical mastitis (swelling, hotness, redness, and apparent milk change) from different dairy farms and veterinary units located at El-Fayoum Governorate during the period from May 2017 to November 2017. Clinical examination proved that out of these animals, a total of 126 animals (87 cattle and 39 buffaloes) were found with clinical mastitis. Streptococcus species were recovered from 73 animals including; 29(39.7%) and 44(60.0%) cows and buffaloes, respectively. Furthermore, out of the 73 Streptococci isolates recovered from cows and buffaloes; there were 10(13.7%) and 15(20.5%) S. agalactiae, 5(6.8%) and 10(23.7%) S. dysgalactiae, 8 (10.6%) and 7 (13.7%) S. uberis, 3(4.1%) and 10(13.7 %) E. fecalis and 3(4.1%) and 2(2.7%) S. lactarius, respectively. Anti-microbial susceptibility testing showed that the highest resistance was recorded against penicillin, gentamicin, streptomycin, and doxycycline (100%). Conversely, the highest sensitivity was recorded against ceftriaxone, ciprofloxacin, and sulfamethoxazole-trimethoprim (100%). Biofilm formation capacity was phenotypically assessed on YESCA CR agar medium and showed that all examined S. agalactiae and S. dysgalactiae were strong biofilm producers, meanwhile, 78%, 50%, and 75% of S. uberis, S. lactarius, and E. fecalis were biofilm positive isolates respectively. Application of PCR technique revealed that enterotoxins producing genes; sed, seb were found in 20% and 80% of isolates, in order. Biofilm-associated genes; fnbA and icaA genes were detected in 90% and 70%, respectively. Resistance genes; mecA and blaZ, genes were possessed in 90% and 70% of isolates, respectively. |
| first_indexed | 2024-04-14T01:06:39Z |
| format | Article |
| id | doaj.art-d7086160a29946b6ac51baecc39a8603 |
| institution | Directory Open Access Journal |
| issn | 2357-0512 2357-0520 |
| language | English |
| last_indexed | 2024-04-14T01:06:39Z |
| publishDate | 2022-04-01 |
| publisher | Faculty of Veterinary Medicine, Beni-Suef University |
| record_format | Article |
| series | Journal of Veterinary Medical Research |
| spelling | doaj.art-d7086160a29946b6ac51baecc39a86032022-12-22T02:21:14ZengFaculty of Veterinary Medicine, Beni-Suef UniversityJournal of Veterinary Medical Research2357-05122357-05202022-04-012901132010.21608/JVMR.2022.121460.1050Phenotypic and Genotypic Characterization of Streptococci Associated with Clinical Bovine MastitisIsmail Raheel0Asmaa Nady Mohammed1Asmaa AbdRabo Mohamed 2Bacteriology, Mycology, Immunology Department Faculty of veterinary medicine Beni-suef University 62511, EgyptDepartment of Hygiene, Zoonoses and Epidemiology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.Directorate of Veterinary Medicine, El-Fayoum Governorate, Egypt.This study was carried out on a total of 550 lactating animals; 310 and 240 cows and buffaloes, respectively which were examined for signs of clinical mastitis (swelling, hotness, redness, and apparent milk change) from different dairy farms and veterinary units located at El-Fayoum Governorate during the period from May 2017 to November 2017. Clinical examination proved that out of these animals, a total of 126 animals (87 cattle and 39 buffaloes) were found with clinical mastitis. Streptococcus species were recovered from 73 animals including; 29(39.7%) and 44(60.0%) cows and buffaloes, respectively. Furthermore, out of the 73 Streptococci isolates recovered from cows and buffaloes; there were 10(13.7%) and 15(20.5%) S. agalactiae, 5(6.8%) and 10(23.7%) S. dysgalactiae, 8 (10.6%) and 7 (13.7%) S. uberis, 3(4.1%) and 10(13.7 %) E. fecalis and 3(4.1%) and 2(2.7%) S. lactarius, respectively. Anti-microbial susceptibility testing showed that the highest resistance was recorded against penicillin, gentamicin, streptomycin, and doxycycline (100%). Conversely, the highest sensitivity was recorded against ceftriaxone, ciprofloxacin, and sulfamethoxazole-trimethoprim (100%). Biofilm formation capacity was phenotypically assessed on YESCA CR agar medium and showed that all examined S. agalactiae and S. dysgalactiae were strong biofilm producers, meanwhile, 78%, 50%, and 75% of S. uberis, S. lactarius, and E. fecalis were biofilm positive isolates respectively. Application of PCR technique revealed that enterotoxins producing genes; sed, seb were found in 20% and 80% of isolates, in order. Biofilm-associated genes; fnbA and icaA genes were detected in 90% and 70%, respectively. Resistance genes; mecA and blaZ, genes were possessed in 90% and 70% of isolates, respectively.https://jvmr.journals.ekb.eg/article_229626_ad1627362ddfcdb4f281935facda324e.pdfbiofilmbovine mastitisresistance genesstreptococcivirulence genes |
| spellingShingle | Ismail Raheel Asmaa Nady Mohammed Asmaa AbdRabo Mohamed Phenotypic and Genotypic Characterization of Streptococci Associated with Clinical Bovine Mastitis Journal of Veterinary Medical Research biofilm bovine mastitis resistance genes streptococci virulence genes |
| title | Phenotypic and Genotypic Characterization of Streptococci Associated with Clinical Bovine Mastitis |
| title_full | Phenotypic and Genotypic Characterization of Streptococci Associated with Clinical Bovine Mastitis |
| title_fullStr | Phenotypic and Genotypic Characterization of Streptococci Associated with Clinical Bovine Mastitis |
| title_full_unstemmed | Phenotypic and Genotypic Characterization of Streptococci Associated with Clinical Bovine Mastitis |
| title_short | Phenotypic and Genotypic Characterization of Streptococci Associated with Clinical Bovine Mastitis |
| title_sort | phenotypic and genotypic characterization of streptococci associated with clinical bovine mastitis |
| topic | biofilm bovine mastitis resistance genes streptococci virulence genes |
| url | https://jvmr.journals.ekb.eg/article_229626_ad1627362ddfcdb4f281935facda324e.pdf |
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