Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses

The objective of this work was to characterize mandarin (Citrus spp.) germplasm from Southern Brazil by morphological and molecular analyses. Thirty seven cultivars from 34 distinct mandarin varieties were evaluated by morphological and agronomic traits of leaves, flowers and fruits, and by microsat...

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Main Authors: Patrícia Koehler-Santos, Ana Lúcia Cunha Dornelles, Loreta Brandão de Freitas
Format: Article
Language:English
Published: Embrapa Informação Tecnológica 2003-07-01
Series:Pesquisa Agropecuária Brasileira
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003000700003&tlng=en
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author Patrícia Koehler-Santos
Ana Lúcia Cunha Dornelles
Loreta Brandão de Freitas
author_facet Patrícia Koehler-Santos
Ana Lúcia Cunha Dornelles
Loreta Brandão de Freitas
author_sort Patrícia Koehler-Santos
collection DOAJ
description The objective of this work was to characterize mandarin (Citrus spp.) germplasm from Southern Brazil by morphological and molecular analyses. Thirty seven cultivars from 34 distinct mandarin varieties were evaluated by morphological and agronomic traits of leaves, flowers and fruits, and by microsatellite markers. The morphological and agronomic characteristics suggested that almost all varieties can be produced for commercial use, and some, as the Satsuma variety, are recommended for breeding programs. Pooled DNA samples from 1-5 plants belonging to each cultivar were tested. Eight of the nine primers detected polymorphisms. Specific markers were found for some accessions. The dendrogram constructed with the morphological results divided the 37 cultivars into four groups, while that obtained with the microsatellites clustered 35 of the 37 cultivars into three groups only. Generally, intervarietal differences are not high, and this lack of agreement in the two multifactorial analyses indicates that diverse evolutionary factors are acting at these two levels of investigation.
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spelling doaj.art-d709411ea1924dd7af12250e7c49f9432022-12-21T21:34:19ZengEmbrapa Informação TecnológicaPesquisa Agropecuária Brasileira1678-39212003-07-0138779780610.1590/S0100-204X2003000700003Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analysesPatrícia Koehler-Santos0Ana Lúcia Cunha Dornelles1Loreta Brandão de Freitas2Universidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulThe objective of this work was to characterize mandarin (Citrus spp.) germplasm from Southern Brazil by morphological and molecular analyses. Thirty seven cultivars from 34 distinct mandarin varieties were evaluated by morphological and agronomic traits of leaves, flowers and fruits, and by microsatellite markers. The morphological and agronomic characteristics suggested that almost all varieties can be produced for commercial use, and some, as the Satsuma variety, are recommended for breeding programs. Pooled DNA samples from 1-5 plants belonging to each cultivar were tested. Eight of the nine primers detected polymorphisms. Specific markers were found for some accessions. The dendrogram constructed with the morphological results divided the 37 cultivars into four groups, while that obtained with the microsatellites clustered 35 of the 37 cultivars into three groups only. Generally, intervarietal differences are not high, and this lack of agreement in the two multifactorial analyses indicates that diverse evolutionary factors are acting at these two levels of investigation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003000700003&tlng=encitrusgenetic variationmicrosatellitesbreeding methods
spellingShingle Patrícia Koehler-Santos
Ana Lúcia Cunha Dornelles
Loreta Brandão de Freitas
Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses
Pesquisa Agropecuária Brasileira
citrus
genetic variation
microsatellites
breeding methods
title Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses
title_full Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses
title_fullStr Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses
title_full_unstemmed Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses
title_short Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses
title_sort characterization of mandarin citrus germplasm from southern brazil by morphological and molecular analyses
topic citrus
genetic variation
microsatellites
breeding methods
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003000700003&tlng=en
work_keys_str_mv AT patriciakoehlersantos characterizationofmandarincitrusgermplasmfromsouthernbrazilbymorphologicalandmolecularanalyses
AT analuciacunhadornelles characterizationofmandarincitrusgermplasmfromsouthernbrazilbymorphologicalandmolecularanalyses
AT loretabrandaodefreitas characterizationofmandarincitrusgermplasmfromsouthernbrazilbymorphologicalandmolecularanalyses