Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses
The objective of this work was to characterize mandarin (Citrus spp.) germplasm from Southern Brazil by morphological and molecular analyses. Thirty seven cultivars from 34 distinct mandarin varieties were evaluated by morphological and agronomic traits of leaves, flowers and fruits, and by microsat...
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Format: | Article |
Language: | English |
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Embrapa Informação Tecnológica
2003-07-01
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Series: | Pesquisa Agropecuária Brasileira |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003000700003&tlng=en |
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author | Patrícia Koehler-Santos Ana Lúcia Cunha Dornelles Loreta Brandão de Freitas |
author_facet | Patrícia Koehler-Santos Ana Lúcia Cunha Dornelles Loreta Brandão de Freitas |
author_sort | Patrícia Koehler-Santos |
collection | DOAJ |
description | The objective of this work was to characterize mandarin (Citrus spp.) germplasm from Southern Brazil by morphological and molecular analyses. Thirty seven cultivars from 34 distinct mandarin varieties were evaluated by morphological and agronomic traits of leaves, flowers and fruits, and by microsatellite markers. The morphological and agronomic characteristics suggested that almost all varieties can be produced for commercial use, and some, as the Satsuma variety, are recommended for breeding programs. Pooled DNA samples from 1-5 plants belonging to each cultivar were tested. Eight of the nine primers detected polymorphisms. Specific markers were found for some accessions. The dendrogram constructed with the morphological results divided the 37 cultivars into four groups, while that obtained with the microsatellites clustered 35 of the 37 cultivars into three groups only. Generally, intervarietal differences are not high, and this lack of agreement in the two multifactorial analyses indicates that diverse evolutionary factors are acting at these two levels of investigation. |
first_indexed | 2024-12-17T20:05:45Z |
format | Article |
id | doaj.art-d709411ea1924dd7af12250e7c49f943 |
institution | Directory Open Access Journal |
issn | 1678-3921 |
language | English |
last_indexed | 2024-12-17T20:05:45Z |
publishDate | 2003-07-01 |
publisher | Embrapa Informação Tecnológica |
record_format | Article |
series | Pesquisa Agropecuária Brasileira |
spelling | doaj.art-d709411ea1924dd7af12250e7c49f9432022-12-21T21:34:19ZengEmbrapa Informação TecnológicaPesquisa Agropecuária Brasileira1678-39212003-07-0138779780610.1590/S0100-204X2003000700003Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analysesPatrícia Koehler-Santos0Ana Lúcia Cunha Dornelles1Loreta Brandão de Freitas2Universidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulUniversidade Federal do Rio Grande do SulThe objective of this work was to characterize mandarin (Citrus spp.) germplasm from Southern Brazil by morphological and molecular analyses. Thirty seven cultivars from 34 distinct mandarin varieties were evaluated by morphological and agronomic traits of leaves, flowers and fruits, and by microsatellite markers. The morphological and agronomic characteristics suggested that almost all varieties can be produced for commercial use, and some, as the Satsuma variety, are recommended for breeding programs. Pooled DNA samples from 1-5 plants belonging to each cultivar were tested. Eight of the nine primers detected polymorphisms. Specific markers were found for some accessions. The dendrogram constructed with the morphological results divided the 37 cultivars into four groups, while that obtained with the microsatellites clustered 35 of the 37 cultivars into three groups only. Generally, intervarietal differences are not high, and this lack of agreement in the two multifactorial analyses indicates that diverse evolutionary factors are acting at these two levels of investigation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003000700003&tlng=encitrusgenetic variationmicrosatellitesbreeding methods |
spellingShingle | Patrícia Koehler-Santos Ana Lúcia Cunha Dornelles Loreta Brandão de Freitas Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses Pesquisa Agropecuária Brasileira citrus genetic variation microsatellites breeding methods |
title | Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses |
title_full | Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses |
title_fullStr | Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses |
title_full_unstemmed | Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses |
title_short | Characterization of mandarin citrus germplasm from Southern Brazil by morphological and molecular analyses |
title_sort | characterization of mandarin citrus germplasm from southern brazil by morphological and molecular analyses |
topic | citrus genetic variation microsatellites breeding methods |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003000700003&tlng=en |
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