Chloroplast Localized FIBRILLIN11 Is Involved in the Osmotic Stress Response during Arabidopsis Seed Germination
Plants live in ever-changing environments, facing adverse environmental conditions including pathogen infection, herbivore attack, drought, high temperature, low temperature, nutrient deficiency, toxic metal soil contamination, high salt, and osmotic imbalance that inhibit overall plant growth and d...
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MDPI AG
2021-04-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/10/5/368 |
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| author | Yu-Ri Choi Inyoung Kim Manu Kumar Jaekyung Shim Hyun-Uk Kim |
| author_facet | Yu-Ri Choi Inyoung Kim Manu Kumar Jaekyung Shim Hyun-Uk Kim |
| author_sort | Yu-Ri Choi |
| collection | DOAJ |
| description | Plants live in ever-changing environments, facing adverse environmental conditions including pathogen infection, herbivore attack, drought, high temperature, low temperature, nutrient deficiency, toxic metal soil contamination, high salt, and osmotic imbalance that inhibit overall plant growth and development. Plants have evolved mechanisms to cope with these stresses. In this study, we found that the <i>FIBRILLIN11</i> (<i>FBN11</i>) gene in Arabidopsis, which has a lipid-binding FBN domain and a kinase domain, is involved in the plant’s response to abiotic stressors, including salt and osmotic stresses. FBN11 protein localizes to the chloroplast. <i>FBN11</i> gene expression significantly changed when plants were exposed to the abiotic stress response mediators such as abscisic acid (ABA), sodium chloride (NaCl), and mannitol. The seed germination rates of <i>fbn11</i> homozygous mutants in different concentrations of mannitol and NaCl were significantly reduced compared to wild type. ABA-dependent and -independent stress response regulatory genes were differentially expressed in the <i>fbn11</i> mutant compared with wild type when grown in mannitol medium. These results suggest a clear role for chloroplast-localized FBN11 in mediating osmotic stress tolerance via the stress response regulatory signaling pathway in the nucleus. |
| first_indexed | 2024-03-10T11:59:20Z |
| format | Article |
| id | doaj.art-d70bc65974af4716b925ece39d30e116 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T11:59:20Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-d70bc65974af4716b925ece39d30e1162023-11-21T17:04:08ZengMDPI AGBiology2079-77372021-04-0110536810.3390/biology10050368Chloroplast Localized FIBRILLIN11 Is Involved in the Osmotic Stress Response during Arabidopsis Seed GerminationYu-Ri Choi0Inyoung Kim1Manu Kumar2Jaekyung Shim3Hyun-Uk Kim4Department of Molecular Biology, Graduate School, Sejong University, Seoul 05006, KoreaDepartment of Molecular Biology, Graduate School, Sejong University, Seoul 05006, KoreaDepartment of Life Science, Dongguk University-Seoul, Ilsandong-gu, Goyang-si 10326, KoreaDepartment of Bioindustry and Bioresource Engineering, Plant Engineering Research Institute, Sejong University, Seoul 05006, KoreaDepartment of Molecular Biology, Graduate School, Sejong University, Seoul 05006, KoreaPlants live in ever-changing environments, facing adverse environmental conditions including pathogen infection, herbivore attack, drought, high temperature, low temperature, nutrient deficiency, toxic metal soil contamination, high salt, and osmotic imbalance that inhibit overall plant growth and development. Plants have evolved mechanisms to cope with these stresses. In this study, we found that the <i>FIBRILLIN11</i> (<i>FBN11</i>) gene in Arabidopsis, which has a lipid-binding FBN domain and a kinase domain, is involved in the plant’s response to abiotic stressors, including salt and osmotic stresses. FBN11 protein localizes to the chloroplast. <i>FBN11</i> gene expression significantly changed when plants were exposed to the abiotic stress response mediators such as abscisic acid (ABA), sodium chloride (NaCl), and mannitol. The seed germination rates of <i>fbn11</i> homozygous mutants in different concentrations of mannitol and NaCl were significantly reduced compared to wild type. ABA-dependent and -independent stress response regulatory genes were differentially expressed in the <i>fbn11</i> mutant compared with wild type when grown in mannitol medium. These results suggest a clear role for chloroplast-localized FBN11 in mediating osmotic stress tolerance via the stress response regulatory signaling pathway in the nucleus.https://www.mdpi.com/2079-7737/10/5/368FIBRILLINkinasechloroplastosmotic stressseed germinationABA signaling pathway |
| spellingShingle | Yu-Ri Choi Inyoung Kim Manu Kumar Jaekyung Shim Hyun-Uk Kim Chloroplast Localized FIBRILLIN11 Is Involved in the Osmotic Stress Response during Arabidopsis Seed Germination Biology FIBRILLIN kinase chloroplast osmotic stress seed germination ABA signaling pathway |
| title | Chloroplast Localized FIBRILLIN11 Is Involved in the Osmotic Stress Response during Arabidopsis Seed Germination |
| title_full | Chloroplast Localized FIBRILLIN11 Is Involved in the Osmotic Stress Response during Arabidopsis Seed Germination |
| title_fullStr | Chloroplast Localized FIBRILLIN11 Is Involved in the Osmotic Stress Response during Arabidopsis Seed Germination |
| title_full_unstemmed | Chloroplast Localized FIBRILLIN11 Is Involved in the Osmotic Stress Response during Arabidopsis Seed Germination |
| title_short | Chloroplast Localized FIBRILLIN11 Is Involved in the Osmotic Stress Response during Arabidopsis Seed Germination |
| title_sort | chloroplast localized fibrillin11 is involved in the osmotic stress response during arabidopsis seed germination |
| topic | FIBRILLIN kinase chloroplast osmotic stress seed germination ABA signaling pathway |
| url | https://www.mdpi.com/2079-7737/10/5/368 |
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