Noninvasive Optical Imaging of Ovarian Metastases Using Cy5-labeled RAFT-c(-RGDfK-)
Our group has developed a new molecular tool based on the use of a regioselectively addressable, functionalized template (RAFT) scaffold, where four cyclic (Arg-Gly-Asp) (cRGD) peptide motifs were grafted. The aim of this study was to determine whether RAFT-c(-RGDfK-) 4 combined with optical imaging...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publications
2006-07-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2006.00022 |
| _version_ | 1797281844202307584 |
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| author | Zhao-hui Jin Veronique Josserand Jesus Razkin Elisabeth Garanger Didier Boturyn Marie-Christine Favrot Pascal Dumy Jean-luc Coll |
| author_facet | Zhao-hui Jin Veronique Josserand Jesus Razkin Elisabeth Garanger Didier Boturyn Marie-Christine Favrot Pascal Dumy Jean-luc Coll |
| author_sort | Zhao-hui Jin |
| collection | DOAJ |
| description | Our group has developed a new molecular tool based on the use of a regioselectively addressable, functionalized template (RAFT) scaffold, where four cyclic (Arg-Gly-Asp) (cRGD) peptide motifs were grafted. The aim of this study was to determine whether RAFT-c(-RGDfK-) 4 combined with optical imaging could allow noninvasive detection of deep ovarian metastases. Human ovarian adenocarcinoma IGROV1 cells expressing low levels of integrin α v β 3 (the main receptor for the cRGD peptide) were used for in vitro and in vivo assays in combination with Cy5-labeled RAFT-c(-RGDfK-) 4 , cRGD, or RAFT-c(-RβADfK-) 4 . In vivo fluorescence imaging was performed on subcutaneous (SC) tumors and intraperitoneal IGROV1 metastases in nude mice. The accumulation of RGD-Cy5 conjugates in cultured cells or in tumor tissues was examined using confocal laser scanning microscopy. RAFT-c(-RGDfK-) 4 exhibited stronger staining in vitro, enhanced tumor-to-background ratio for SC tumors, and allowed early detection of 1- to 5-mm large intraabdominal nodules using noninvasive optical imaging. Histological study revealed that RAFT-c(-RGDfK-) 4 accumulated into tumor neovasculature but also into tumor cells. Our data demonstrate that a Cy5-labeled RAFT-c(-RGDfK-) 4 is an efficient optical probe for early and noninvasive tumor detection. |
| first_indexed | 2024-03-07T17:03:18Z |
| format | Article |
| id | doaj.art-d70c0cc6e34f492da63644d1382e212c |
| institution | Directory Open Access Journal |
| issn | 1536-0121 |
| language | English |
| last_indexed | 2024-03-07T17:03:18Z |
| publishDate | 2006-07-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj.art-d70c0cc6e34f492da63644d1382e212c2024-03-03T03:12:23ZengSAGE PublicationsMolecular Imaging1536-01212006-07-01510.2310/7290.2006.0002210.2310_7290.2006.00022Noninvasive Optical Imaging of Ovarian Metastases Using Cy5-labeled RAFT-c(-RGDfK-)Zhao-hui Jin0Veronique Josserand1Jesus Razkin2Elisabeth Garanger3Didier Boturyn4Marie-Christine Favrot5Pascal Dumy6Jean-luc Coll7Institut Albert Bonniot, FranceInstitut Albert Bonniot, FranceUniversité Joseph Fourier, FranceUniversité Joseph Fourier, FranceUniversité Joseph Fourier, FranceInstitut Albert Bonniot, FranceUniversité Joseph Fourier, FranceInstitut Albert Bonniot, FranceOur group has developed a new molecular tool based on the use of a regioselectively addressable, functionalized template (RAFT) scaffold, where four cyclic (Arg-Gly-Asp) (cRGD) peptide motifs were grafted. The aim of this study was to determine whether RAFT-c(-RGDfK-) 4 combined with optical imaging could allow noninvasive detection of deep ovarian metastases. Human ovarian adenocarcinoma IGROV1 cells expressing low levels of integrin α v β 3 (the main receptor for the cRGD peptide) were used for in vitro and in vivo assays in combination with Cy5-labeled RAFT-c(-RGDfK-) 4 , cRGD, or RAFT-c(-RβADfK-) 4 . In vivo fluorescence imaging was performed on subcutaneous (SC) tumors and intraperitoneal IGROV1 metastases in nude mice. The accumulation of RGD-Cy5 conjugates in cultured cells or in tumor tissues was examined using confocal laser scanning microscopy. RAFT-c(-RGDfK-) 4 exhibited stronger staining in vitro, enhanced tumor-to-background ratio for SC tumors, and allowed early detection of 1- to 5-mm large intraabdominal nodules using noninvasive optical imaging. Histological study revealed that RAFT-c(-RGDfK-) 4 accumulated into tumor neovasculature but also into tumor cells. Our data demonstrate that a Cy5-labeled RAFT-c(-RGDfK-) 4 is an efficient optical probe for early and noninvasive tumor detection.https://doi.org/10.2310/7290.2006.00022 |
| spellingShingle | Zhao-hui Jin Veronique Josserand Jesus Razkin Elisabeth Garanger Didier Boturyn Marie-Christine Favrot Pascal Dumy Jean-luc Coll Noninvasive Optical Imaging of Ovarian Metastases Using Cy5-labeled RAFT-c(-RGDfK-) Molecular Imaging |
| title | Noninvasive Optical Imaging of Ovarian Metastases Using Cy5-labeled RAFT-c(-RGDfK-) |
| title_full | Noninvasive Optical Imaging of Ovarian Metastases Using Cy5-labeled RAFT-c(-RGDfK-) |
| title_fullStr | Noninvasive Optical Imaging of Ovarian Metastases Using Cy5-labeled RAFT-c(-RGDfK-) |
| title_full_unstemmed | Noninvasive Optical Imaging of Ovarian Metastases Using Cy5-labeled RAFT-c(-RGDfK-) |
| title_short | Noninvasive Optical Imaging of Ovarian Metastases Using Cy5-labeled RAFT-c(-RGDfK-) |
| title_sort | noninvasive optical imaging of ovarian metastases using cy5 labeled raft c rgdfk |
| url | https://doi.org/10.2310/7290.2006.00022 |
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