The role of enzymes in the angiogenic activity of Hancornia speciosa latex
The Hancornia speciosa latex has shown angiogenic activity. Angiogenesis plays a major role in wound healing, and materials that stimulate this process could be used to develop drugs. This study aimed to explain the role of proteins in the H. speciosa serum fraction latex in angiogenesis. Hence, thi...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Uberlândia
2022-09-01
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| Series: | Bioscience Journal |
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| Online Access: | https://seer.ufu.br/index.php/biosciencejournal/article/view/61092 |
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| author | Patricia D'Abadia Amanda Fernandes Costa Myllena Tolentino Firmino Firmino Samantha Caramori Ruy de Souza Lino-Junior Fabiana Fonseca Zanoelo Zanoelo Pablo José Gonçalves Gonçalves Luciane Almeida |
| author_facet | Patricia D'Abadia Amanda Fernandes Costa Myllena Tolentino Firmino Firmino Samantha Caramori Ruy de Souza Lino-Junior Fabiana Fonseca Zanoelo Zanoelo Pablo José Gonçalves Gonçalves Luciane Almeida |
| author_sort | Patricia D'Abadia |
| collection | DOAJ |
| description | The Hancornia speciosa latex has shown angiogenic activity. Angiogenesis plays a major role in wound healing, and materials that stimulate this process could be used to develop drugs. This study aimed to explain the role of proteins in the H. speciosa serum fraction latex in angiogenesis. Hence, this material was treated with proteinase K and the proteins were inactivated. After protein inactivation, angiogenic activity was assessed with the chick chorioallantoic membrane assay. The result showed that the proteins in the serum fraction are responsible for angiogenic activity. Then, the total protein content in the serum fraction and its enzymatic activity were investigated. The low protein content observed in the H. speciosa serum fraction latex suggests that this biomaterial could be used to develop new drugs with a hypoallergenic response. Despite the low protein content, there was a significant enzymatic activity of at least three enzymes in the serum fraction latex: β-1,3 glucanase, β-glucosidase, and proteases. These enzymes seem to influence the healing process, assisting debridement, extracellular matrix remodeling, and collagen deposition, and decreasing the chances of contamination by microorganisms. In conclusion, the enzymes in the H. speciosa serum latex are associated with the angiogenic activity of this biomaterial and may be used to assist the wound healing process. |
| first_indexed | 2024-04-11T09:16:26Z |
| format | Article |
| id | doaj.art-d70c6cdf3e9d4b13b5251c71f1324cc9 |
| institution | Directory Open Access Journal |
| issn | 1981-3163 |
| language | English |
| last_indexed | 2024-04-11T09:16:26Z |
| publishDate | 2022-09-01 |
| publisher | Universidade Federal de Uberlândia |
| record_format | Article |
| series | Bioscience Journal |
| spelling | doaj.art-d70c6cdf3e9d4b13b5251c71f1324cc92022-12-22T04:32:19ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632022-09-0138e38086e3808610.14393/BJ-v38n0a2022-6109233709The role of enzymes in the angiogenic activity of Hancornia speciosa latexPatricia D'Abadia0https://orcid.org/0000-0003-4756-4869Amanda Fernandes Costa1https://orcid.org/0000-0002-1768-6083Myllena Tolentino Firmino Firmino2https://orcid.org/0000-0002-2807-4760Samantha Caramori3https://orcid.org/0000-0003-2676-8280Ruy de Souza Lino-Junior4https://orcid.org/0000-0002-0572-5102Fabiana Fonseca Zanoelo Zanoelo5https://orcid.org/0000-0002-5778-0322Pablo José Gonçalves Gonçalves6https://orcid.org/0000-0003-0246-1073Luciane Almeida7https://orcid.org/0000-0003-1764-1480Universidade Estadual de GoiásUniversidade Estadual de GoiásUniversidade Estadual de GoiásUniversidade Estadual de GoiásUniversidade Federal de GoiásUniversidade Federal do Mato Grosso do SulUniversidade Federal de GoiásUniversidade Estadual de GoiásThe Hancornia speciosa latex has shown angiogenic activity. Angiogenesis plays a major role in wound healing, and materials that stimulate this process could be used to develop drugs. This study aimed to explain the role of proteins in the H. speciosa serum fraction latex in angiogenesis. Hence, this material was treated with proteinase K and the proteins were inactivated. After protein inactivation, angiogenic activity was assessed with the chick chorioallantoic membrane assay. The result showed that the proteins in the serum fraction are responsible for angiogenic activity. Then, the total protein content in the serum fraction and its enzymatic activity were investigated. The low protein content observed in the H. speciosa serum fraction latex suggests that this biomaterial could be used to develop new drugs with a hypoallergenic response. Despite the low protein content, there was a significant enzymatic activity of at least three enzymes in the serum fraction latex: β-1,3 glucanase, β-glucosidase, and proteases. These enzymes seem to influence the healing process, assisting debridement, extracellular matrix remodeling, and collagen deposition, and decreasing the chances of contamination by microorganisms. In conclusion, the enzymes in the H. speciosa serum latex are associated with the angiogenic activity of this biomaterial and may be used to assist the wound healing process.https://seer.ufu.br/index.php/biosciencejournal/article/view/61092chick chorioallantoic membraneenzymesproteinase k |
| spellingShingle | Patricia D'Abadia Amanda Fernandes Costa Myllena Tolentino Firmino Firmino Samantha Caramori Ruy de Souza Lino-Junior Fabiana Fonseca Zanoelo Zanoelo Pablo José Gonçalves Gonçalves Luciane Almeida The role of enzymes in the angiogenic activity of Hancornia speciosa latex Bioscience Journal chick chorioallantoic membrane enzymes proteinase k |
| title | The role of enzymes in the angiogenic activity of Hancornia speciosa latex |
| title_full | The role of enzymes in the angiogenic activity of Hancornia speciosa latex |
| title_fullStr | The role of enzymes in the angiogenic activity of Hancornia speciosa latex |
| title_full_unstemmed | The role of enzymes in the angiogenic activity of Hancornia speciosa latex |
| title_short | The role of enzymes in the angiogenic activity of Hancornia speciosa latex |
| title_sort | role of enzymes in the angiogenic activity of hancornia speciosa latex |
| topic | chick chorioallantoic membrane enzymes proteinase k |
| url | https://seer.ufu.br/index.php/biosciencejournal/article/view/61092 |
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